Primary antibodies used for immunostaining were anti-TRF1 (Abcam, ab192629, 1:100), anti-BubR1 (Abcam, ab28193, 1:100), anti-Zw10 (Abcam, ab21582, 1:100), anti-acetylated-α-tubulin (Sigma Aldrich, T7451, 1:500; Abcam, ab179484, 1:500), anti-centromere (Antibodies Incorporated, 15-234, 1:100), anti-CENP-A (Cell Signaling, #2048, 1:100), anti-Survivin (Cell Signaling, #2808, 1:100), anti-Hec1 (Santa Cruz Biotechnology, sc-515550, 1:100), anti-H3T3ph (Upstate, 07-424, 1:100), anti-SMC3 (Abcam, ab128919, 1:100), anti-SMC4 (Novus Biologicals, NBP1-86635, 1:100), and anti-TRF2 (Abcam, ab13579, 1:100). Secondary antibodies were Alexa Fluor 488-conjugated anti-mouse (Jackson ImmunoResearch, 115-545-144 1:500), Alexa Fluor 594-conjugated anti-mouse (Jackson ImmunoResearch, 111-585-146, 1:500), Alexa Fluor 488-conjugated anti-rabbit (Jackson ImmunoResearch, 115-545-144 1:500), Alexa Fluor 594-conjugated anti-rabbit (Jackson ImmunoResearch, 111-585-144, 1:500), and Alexa Fluor 488-conjugated anti-sheep antibodies (Abcam, ab150177, 1:500).
Ab128919
Manufactured by Novus Biologicals
Ab128919 is a laboratory equipment product. It is a device used for scientific research and experimentation purposes.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 conjugated anti rabbit, by Jackson ImmunoResearch (2 mentions)
Alexa fluor 488 conjugated anti mouse, by Jackson ImmunoResearch (2 mentions)
Alexafluor 594 conjugated anti rabbit, by Jackson ImmunoResearch (2 mentions)
Ab13579, by Abcam (1 mentions)
Ab28193, by Abcam (1 mentions)
Anti survivin, by Cell Signaling Technology (1 mentions)
Ab179484, by Abcam (1 mentions)
Ab22551, by Abcam (1 mentions)
Ab70383, by Abcam (1 mentions)
T7451, by Abcam (1 mentions)
2 protocols using ab128919
1
Immunostaining of Mitotic Proteins
2
Immunofluorescence Staining of Mitotic Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following primary antibodies were used: anti‐γ‐H2AX (Abcam, ab22551, 1:250), anti‐MDC1 (Abcam, ab241048, 1:100), anti‐acetylated‐α‐tubulin (Sigma‐Aldrich, T7451, 1:500; Abcam, ab179484, 1:500), anti‐Mad2 (Abcam, ab70383, 1:100), anti‐BubR1 (Abcam, ab28193, 1:100), anti‐centromere (ACA; Antibodies Incorporated, 15–234, 1:100), anti‐SMC3 (Abcam, ab128919, 1:100), anti‐SMC4 (Novus Biologicals, NBP1‐86635, 1:100) and anti‐CENP‐A (Cell Signaling, #2048, 1:250). Alexa Fluor 488‐conjugated anti‐mouse (Jackson ImmunoResearch, 115‐545‐144, 1:500), Alexa Fluor 488‐conjugated anti‐rabbit (Jackson ImmunoResearch, 115‐545‐144 1:500), Alexa Fluor 594‐conjugated anti‐rabbit (Jackson ImmunoResearch, 111‐585‐144, 1:500) and Rhodamine (TRITC) ‐conjugated anti‐human (Jackson ImmunoResearch, 109‐025‐088, 1:100) were used as secondary antibodies.
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