Hdac fluorometric assay drug discovery kit

Manufactured by Enzo Life Sciences

Sourced in United States

The HDAC Fluorometric Assay/Drug Discovery kit is a laboratory tool designed to measure the activity of histone deacetylase (HDAC) enzymes. It utilizes a fluorogenic substrate to enable the quantitative detection of HDAC activity, which is a key aspect of epigenetic regulation and drug discovery research.

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Fluorometric assay kit (2 citations)

2 protocols using hdac fluorometric assay drug discovery kit

HDAC Activity Assay Protocol

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HDAC activity assays were performed as previously described.^{24 (link), 25 (link)} HDAC activity was determined using a commercial assay kit from Enzo Life Sciences (HDAC Fluorometric Assay/Drug Discovery kit, Farmingdale, NY, USA). For the HDAC1 activity assay, cells were transfected with HDAC1 WT or the HDAC1 2R mutant and the SUMO1 construct, followed by immunoprecipitation using an anti-Flag antibody.

Joung H., Kwon S., Kim K.H., Lee Y.G., Shin S., Kwon D.H., Lee Y.U., Kook T., Choe N., Kim J.C., Kim Y.K., Eom G.H, & Kook H. (2018). Sumoylation of histone deacetylase 1 regulates MyoD signaling during myogenesis. Experimental & Molecular Medicine, 50(1), e427-.

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HDAC Activity Determination Assay

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HDAC1 (BML-AK511), HDAC6 (BML-AK516) and HDAC8 (BML-AK518) activity was determined using a commercial assay kit from Enzo Life Sciences (HDAC fluorometric assay/drug discovery kit, Farmingdale, NY, USA). The assay was carried out according to the manufacturer’s instructions. Fluorescence intensity was measured at an excitation wavelength of 360 nm and an emission wavelength of 460 nm using a fluorometer (LS 55, Perkin Elmer, USA). Trichostatin A (TSA) was also evaluated as a positive control. IC₅₀ values were determined using GraphPad Prism 5 software.

Sixto-López Y., Gómez-Vidal J.A., de Pedro N., Bello M., Rosales-Hernández M.C, & Correa-Basurto J. (2020). Hydroxamic acid derivatives as HDAC1, HDAC6 and HDAC8 inhibitors with antiproliferative activity in cancer cell lines. Scientific Reports, 10, 10462.

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