Fluorescent images were taken on a Leica TCS SP2 confocal microscope and entire images were processed with Adobe Photoshop software; images are all from a single xy stack. ImageJ free software from NIH, Bethesda, MD, USA was used to rebuild the projections along the z axis starting from 35–50 xy stacks. Unless otherwise specified, each experiment is based on the observation of 55–80 wing discs, and all figures show a phenotype found in over 75% of the samples, that we consider highly representative.
Tcs sp2 confocal microscope
Manufactured by Adobe
The TCS SP2 is a confocal microscope designed for high-resolution imaging. It features a scanning laser system that allows for the acquisition of optical sections, enabling the reconstruction of three-dimensional images. The microscope is equipped with multiple laser sources and detectors, providing flexibility for a range of fluorescent applications.
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Lab products found in correlation
2 protocols using tcs sp2 confocal microscope
1
Imaging Wing Disc Development
2
Confocal Microscopy and Image Analysis
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Fluorescent images were taken on a Leica TCS SP2 confocal microscope, and the entire images were processed with Adobe Photoshop software; all the images shown are from a single z stack. ImageJ free software from NIH, Bethesda, MD, USA, was used to measure sample diameter and area. For statistical analysis, the number of samples is indicated in the figures. For IF analysis, the figures represent the average phenotype across 15–25 samples analysed, if not otherwise specified. Data represent mean ± s.d. Two-tailed Student's t-tests were used to determine significance. ∗∗P < 0.01. Graphs were created in GraphPad Prism 5.
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