Pre-coated ELISA kits (Biolegend) were used to assess the relative amounts of TNF-α, IL-6 and IFN-γ in the serum from rats that self-administered methamphetamine (n = 18) and saline controls (n = 16). Samples were thawed on assay days and run twice in duplicate following manufacturer instructions. Concentrations of each cytokine were plotted against a standard curve fitting of the corresponding cytokine using Excel (Microsoft).
Pre coated elisa kits
Manufactured by BioLegend
Sourced in United States
Pre-coated ELISA kits are laboratory equipment used to detect and quantify specific proteins or other analytes in biological samples. They come with the capture and detection antibodies pre-immobilized on the plate, streamlining the assay setup process.
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Lab products found in correlation
3 protocols using pre coated elisa kits
1
Cytokine Profiling in Methamphetamine Use
2
Multiplex Cytokine Quantification
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Multiplex fluorescent bead-based immunoassays were used to measure IL-17A and IFN-g (Diaclone SAS, Besanc ¸on, France) and IL-17F (BD Biosciences, Franklin Lakes, NJ) concentration in collected culture supernatants. IL-9 concentration was quantified by using precoated ELISA kits (BioLegend, San Diego, CA).
3
Multiplex Cytokine Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Multiplex fluorescent bead-based immunoassays were used to measure IL-17A and IFN-γ (Diaclone SAS, Besançon, France) and IL-17F (BD Bioscience, Franklin Lakes, NJ, USA) concentration in collected culture supernatants. IL-9 concentration was quantified by using pre-coated ELISA kits (Biolegend, San Diego, CA, USA).
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