The largest database of trusted experimental protocols

Thy broth

Manufactured by Merck Group
Sourced in United States

THY broth is a culture medium used for the growth and maintenance of various microorganisms. It provides the necessary nutrients and growth factors required for the cultivation of these organisms in a laboratory setting.

Automatically generated - may contain errors

2 protocols using thy broth

1

Streptococcus pyogenes Culturing and Manipulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Streptococcus pyogenes M1T1 strain 5448 (accession: CP008776.1) was isolated from a patient with toxic shock syndrome and necrotizing fasciitis that is genetically representative of a globally disseminated clone associated with invasive S. pyogenes infections (Kansal et al., 2000 (link)). S. pyogenes strains were grown at 37°C in a screw-cap glass tube (Pyrex; Iwaki Glass, Tokyo, Japan) filled with Todd-Hewitt broth (BD Biosciences, San Jose, CA, USA) supplemented with 0.2% yeast extract (BD Bioscience) (THY broth) in an ambient atmosphere and standing cultures. To obtain cultures for experiments and observe pH change, overnight cultures of S.pyogenes were back diluted 1:50 into fresh THY broth or phenol red broth (Sigma Aldrich, St Louis, MO, USA) supplemented with 30 mM arginine. CFUs were determined by plating diluted samples on THY blood agar.
Escherichia coli strain XL-10 Gold (Agilent Technologies, Santa Clara, CA, USA) was used as a host for derivatives of plasmids pSET4s (Takamatsu et al., 2001 (link)) and pQE30 (Qiagen, Hilden, Germany). E. coli strains were cultured in Luria-Bertani medium (Nacalai Tesque, Kyoto, Japan) at 37°C with agitation. For selection and maintenance of strains, antibiotics were added to the medium at the following concentrations: spectinomycin, 100 μg/mL for S. pyogenes and E. coli: carbenicillin, 100 μg/mL for E. coli.
+ Open protocol
+ Expand
2

UV-Inactivation of S. pneumoniae TIGR4

Check if the same lab product or an alternative is used in the 5 most similar protocols
Capsular serotype 4 S. pneumoniae TIGR4 (a gift from Professor Jeffrey Weiser, University of Pennsylvania) was grown overnight on Columbia agar blood plate (SGL, 8022) at 37 °C in 5% CO2, then transferred into 15mls of autoclaved THY broth (Sigma Aldrich, T1438) supplemented with 0.5% yeast extract (Sigma Aldrich, Y1625) in a 50 ml falcon tube (Greiner, T2318-500EA) and cultured at 37 °C in 5% CO2 with the cap loosely replaced. On reaching an optical density600 of 0.4 (approximately 1 × 108 colony forming units [CFU] per ml) the S. pneumoniae cultures were centrifuged at 13,000×g for 10 min, the supernatant discarded, and bacteria re-suspended in sterile PBS (Sigma Aldrich, D8537) in a sterile petri dish then exposed to UV light (302 nm, ChemiDoc; Bio-Rad, UK) for 1 h. The UV-killed S. pneumoniae were collected into a sterile 50 ml falcon tube, washed with sterile normal saline and centrifugation at 13,000×g for 10 min. Aliquots of 1.5 × 108 UV-killed S. pneumoniae in 1 ml of sterile saline were frozen at −80 °C in autoclaved 10% glycerol (VWR 24,388.260) diluted in distilled water until required for injection. The S. pneumoniae in the UV stocks were confirmed to be dead by culture (probable limit of detection of < 1 in 1 million bacteria) by the University College London Hospitals Microbiology department.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!