Escherichia coli strain XL-10 Gold (Agilent Technologies, Santa Clara, CA, USA) was used as a host for derivatives of plasmids pSET4s (Takamatsu et al., 2001 (link)) and pQE30 (Qiagen, Hilden, Germany). E. coli strains were cultured in Luria-Bertani medium (Nacalai Tesque, Kyoto, Japan) at 37°C with agitation. For selection and maintenance of strains, antibiotics were added to the medium at the following concentrations: spectinomycin, 100 μg/mL for S. pyogenes and E. coli: carbenicillin, 100 μg/mL for E. coli.
Thy broth
THY broth is a culture medium used for the growth and maintenance of various microorganisms. It provides the necessary nutrients and growth factors required for the cultivation of these organisms in a laboratory setting.
Lab products found in correlation
2 protocols using thy broth
Streptococcus pyogenes Culturing and Manipulation
UV-Inactivation of S. pneumoniae TIGR4
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