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Percp cy5.5 7 aminoactinomycin d 7 aad

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PerCP/Cy5.5-7-aminoactinomycin D (7-AAD) is a fluorescent dye that binds to DNA. It can be used to detect cell viability and apoptosis in flow cytometry applications.

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Lab products found in correlation

Novocyte flow cytometer, by Agilent Technologies (1 mentions) Cd40 apc, by Thermo Fisher Scientific (1 mentions) Cd11c apc cy7, by BioLegend (1 mentions) Cd207 pe, by BioLegend (1 mentions) Acea novoexpress software, by Agilent Technologies (1 mentions) Mhcii fitc, by BioLegend (1 mentions) Cd45 bv510, by BioLegend (1 mentions) Cd11b apc, by Thermo Fisher Scientific (1 mentions) Bv711 cd64, by BioLegend (1 mentions) Ly6c apc cy7, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions)

2 protocols using percp cy5.5 7 aminoactinomycin d 7 aad

1

Dendritic Cell Immunophenotyping by Flow Cytometry

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The cells were washed and resuspended in PBS. The single-cell suspensions were stained with the following antibodies: CD207-PE (144204, BioLegend, San Diego, CA, USA), FITC-MHCII (I-A-I-E, 107606, BioLegend, San Diego, CA, USA), APC/Cy7-CD11c (117324, BioLegend, San Diego, CA, USA), PE/Cy7-CD86 (25-0862-82, eBioscience, Carlsbad, USA), APC-CD40 (17-0401-82, eBioscience, Carlsbad, USA), PerCP/Cy5.5-7-aminoactinomycin D (7-AAD) (BioLegend, 420404; 0.5 μg/ml, San Diego, CA, USA). Flow cytometry was performed using the NovoCyte flow cytometer and ACEA NovoExpress™ software (ACEA Biosciences, San Diego, CA, USA).
Zhao F., Zhang C., Li G., Zheng H., Gu L., Zhou H., Xiao Y., Wang Z., Yu J., Hu Y., Zeng F., Wang X., Zhao Q., Hu J., Yue C., Zhou P., Huang N., Hao Y., Wu W., Cui K., Li W, & Li J. (2022). A role for whey acidic protein four-disulfide-core 12 (WFDC12) in the pathogenesis and development of psoriasis disease. Frontiers in Immunology, 13, 873720.
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2

Multiparametric Flow Cytometry Analysis

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The cells were washed and resuspended in PBS. The single-cell suspensions were stained with the following antibodies: PerCP/Cy5.5-7-aminoactinomycin D (7-AAD) (BioLegend, 420404; 0.5 μg/ml, San Diego, CA, USA), APC-CD11b (17-0112-82, eBioscience, Carlsbad, USA), APC/Cy7-Ly6C (1208026, BioLegend, San Diego, CA, USA), BV510-CD45 (1033137, BioLegend, San Diego, CA, USA), BV711-CD64 (139311, BioLegend, San Diego, CA, USA), FITC-MHCII, PE-MerTK (151506, BioLegend, San Diego, CA, USA).
The BD LSRFortessaTM and Flow Jo™ software (BD Biosciences, Franklin. Lakes, NJ, USA) were used for flow cytometry analysis.
Zhao F., Zhang C., Li G., Zheng H., Gu L., Zhou H., Xiao Y., Wang Z., Yu J., Hu Y., Zeng F., Wang X., Zhao Q., Hu J., Yue C., Zhou P., Huang N., Hao Y., Wu W., Cui K., Li W, & Li J. (2022). A role for whey acidic protein four-disulfide-core 12 (WFDC12) in the pathogenesis and development of psoriasis disease. Frontiers in Immunology, 13, 873720.
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