Cells cultured as a confluent monolayer on glass coverslips were fixed with 4% paraformaldehyde. Permeabilization was performed with 0.1% Triton-X (Sigma-Aldrich #T8787) and a blocking step was carried out with 4% swine serum (Jackson ImmunoResearch, #014-000-121) for 1 hr. Primary and secondary antibodies were diluted in 4% swine serum as stated in the key resource table and incubated at room temperature for 1 hr or at 4°C overnight. Cell nuclei were stained with DAPI (Sigma-Aldrich #D9542). Imaging was carried out with Leica TCS SP8 X DLS confocal microscope. Data visualization and processing was performed with the Leica LAS-X software.
Tcs sp8 x dls confocal microscope
Manufactured by Leica
The Leica TCS SP8 X DLS confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It features a spectral detection system that enables the simultaneous acquisition of multiple fluorescence signals. The microscope is equipped with a tunable white-light laser, providing a wide range of excitation wavelengths for flexibility in experimental design. The system also incorporates a Digital Light Stimulation (DLS) module, allowing for precise control and manipulation of light within the sample.
Automatically generated - may contain errors
2 protocols using tcs sp8 x dls confocal microscope
1
Immunofluorescence Staining of Cell Monolayer
2
Immunofluorescence Labeling of Cell Cultures
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Cells cultured as a confluent monolayer on glass coverslips were fixed with 4% paraformaldehyde. Permeabilization was performed with 0.1% Triton-X (Sigma Aldrich #T8787) and a blocking step was carried out with 4% swine serum (Jackson Immunoresearch, #014-000-121) for one hour. Primary and secondary antibodies were diluted in 4% swine serum as stated in Table S4 and incubated at room temperature for 1 hour or at 4 °C overnight.
Cell nuclei were stained with DAPI (Sigma Aldrich #D9542). Imaging was carried out with Leica TCS SP8 X DLS confocal microscope. Data visualization and processing was performed with the Leica LAS-X software.
Cell nuclei were stained with DAPI (Sigma Aldrich #D9542). Imaging was carried out with Leica TCS SP8 X DLS confocal microscope. Data visualization and processing was performed with the Leica LAS-X software.
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