Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti collagen 1 antibody

Manufactured by Bioss Antibodies
Visit Supplier
Sourced in China

Rabbit anti-collagen I antibody is a laboratory reagent designed for the detection and analysis of collagen type I. It is a polyclonal antibody raised in rabbits against collagen I, a major structural protein found in connective tissues. This antibody can be used in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry, to identify and quantify collagen I in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Methacholine, by Merck Group (1 mentions) Tiotropium, by Merck Group (1 mentions) Mouse anti alpha smooth muscle actin antibody, by Abcam (1 mentions)

2 protocols using rabbit anti collagen 1 antibody

1

Immunoblotting Analysis of Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
HRP-conjugated goat anti-rabbit antibody and HRP- conjugated goat anti-mouse antibody were purchased from FDbio science. Mouse anti-alpha smooth muscle actin antibody was from Abcam (Cambridge, MA, USA). Rabbit anti-phospho-GSK3β antibody, rabbit anti-total β-catenin antibody, rabbit anti-nonphosphorylated- β-catenin antibody were from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-GSK3(α+β) antibody was from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Rabbit anti-collagen I antibody was from Bioss (Beijing, China). Methacholine and tiotropium were from Sigma-Aldrich Co. (St Louis, MO, USA).
Huo Y., Guan L., Xu J., Zhou L, & Chen R. (2018). Tiotropium inhibits methacholine-induced extracellular matrix production via β-catenin signaling in human airway smooth muscle cells. International Journal of Chronic Obstructive Pulmonary Disease, 13, 1469-1481.
+ Open protocol
+ Expand
2

Immunostaining of Collagen I in Formalin-Fixed Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunostaining was performed on formalin-xed, para n-embedded tissue. For immunohistochemistry, para n sections were dewaxed in xylene, rehydrated with distilled water, and then subjected to antigen retrieval for 30 min at 95°C. The slides were subsequently incubated overnight at 4°C with rabbit anti-Collagen I antibody (1:400,bioss,Beijing,China).Slides were then treated with an anti-rabbit secondary antibody(Zhongshanjinqiao , Beijing, China)and developed using avidin-conjugated HRP with diaminobenzidine as a substrate (Zhongshanjinqiao , Beijing, China),followed by hematoxylin counter staining.
He J., Qin M., Chen Y., Hu Z., Ye L, & Hui T. (2021). EZH2 Promotes Extracellular Matrix Degradation via Nuclear Factor-κB (NF-κB) and p38 Signaling Pathways in Pulpitis. Inflammation, 44(5).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!