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F 7000 uorescence spectrometer

Manufactured by Hitachi

The F-7000 fluorescence spectrometer is a laboratory instrument manufactured by Hitachi. It is designed to measure and analyze the fluorescence properties of various samples. The core function of the F-7000 is to excite samples with a light source and detect the resulting fluorescence emission, providing quantitative data on the fluorescence characteristics of the sample.

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2 protocols using f 7000 uorescence spectrometer

1

Synthesis and Characterization of a Fluorescent Coumarin Probe

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All chemicals were obtained from commercial suppliers and directly used without further puri cation. 7-(diethylamino)coumarin-3-carbohydrazide was prepared according to the literature method [16] .
Analytical grade acetonitrile and deionized water were used as solvents for spectral measurements. 1 H NMR and 13 C NMR spectra were recorded on a Bruker Av400 NMR spectrometer. HRMS spectra were performed on a Shimadzu LCMS-IT-TOF apparatus. Fluorescence spectra were taken on a Hitachi F-7000 uorescence spectrometer. The X-ray diffraction data was collected on an Agilent Gemini A Ultra diffractometer (MoKα, λ = 0.71073 Å). Images of cells were obtained using a Nikon A1 confocal laser scanning microscope.
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2

Characterization of a Novel Compound

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All chemicals were obtained from commercial suppliers. 1 H NMR and 13 C NMR spectra were recorded on a Bruker Av400 NMR spectrometer. ESI-MS spectra were performed on a Bruker Esquire HCT mass spectrometer. Fluorescence spectra were taken on a Hitachi F-7000 uorescence spectrometer. The X-ray diffraction data was collected on an Agilent Gemini A Ultra diffractometer. Images of cells were obtained using a Nikon A1 confocal laser scanning microscope.
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