Iron(iii ) chloride hexahydrate (FeCl3·6H2O), iron(ii ) chloride tetrahydrate (FeCl2·4H2O), graphite nanopowder (type-1), paraffin liquid oil (extrapure), potassium permanganate (KMnO4), ammonium hydroxide (NH4OH), concentrated sulphuric acid (H2SO4), hydrogen peroxide (H2O2), sodium nitrate (NaNO3), potassium chloride (KCl), potassium ferricyanide ([K3Fe(CN)6]) extrapure 98%, agarose (low EEO), CaCl2 fused pure (90–95%), sucrose, sodium borohydride powder (NaBH4), magnesium sulphate dried extrapure (MgSO4·xH2O), sodium phosphate monobasic dihydrate extrapure (NaH2PO4·2H2O) and sodium bicarbonate extrapure (NaHCO3) were purchased from SRL chemicals. Dopamine hydrochloride, uric acid, acetic acid and serotonin hydrochloride were purchased from Sigma-Aldrich. Chitosan (0.5% in 0.5% acetic acid) obtained from Tokyo Chemical Industry (TCI Chemicals). Glucose (d -glucose anhydrous) was purchased from Nice Chemicals. All the mentioned chemicals were used for analysis without any further purifications.
Sodium nitrate
Manufactured by Sisco Research Laboratories
Sourced in United States, India
Sodium nitrate is an inorganic compound with the chemical formula NaNO3. It is a white, crystalline solid that is commonly used in various industrial and agricultural applications.
Automatically generated - may contain errors
Lab products found in correlation
Potassium permanganate, by Sisco Research Laboratories (2 mentions)
Serotonin hydrochloride, by Merck Group (1 mentions)
Uric acid, by Merck Group (1 mentions)
Chitosan, by Tokyo Chemical Industry (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Dopamine hydrochloride, by Merck Group (1 mentions)
Ammonium hydroxide, by Sisco Research Laboratories (1 mentions)
Potassium chloride (kcl), by Sisco Research Laboratories (1 mentions)
Sucrose, by Sisco Research Laboratories (1 mentions)
Graphite flakes, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Xanthine, by Merck Group (1 mentions)
Xanthine oxidase, by Merck Group (1 mentions)
Tris hcl, by Merck Group (1 mentions)
Lps l4391, by Merck Group (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Cytochrome c, by Merck Group (1 mentions)
3 protocols using sodium nitrate
1
Synthesis and Characterization of Iron Oxide Nanocomposites
2
Graphene Oxide Synthesis and Characterization
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Graphene oxide
(GO) was synthesized using the modified Hummers method
followed by sonication.31 Briefly, graphite
flakes (Merck, USA), sodium nitrate, and potassium permanganate (SRL
Chemicals, Mumbai, India) were mixed at a ratio of 2:1:6 in the presence
of concentrated H2SO4 in an ice bath (at 0–5
°C). The reaction mixture was diluted by adding distilled water
under vigorous stirring followed by addition of hydrogen peroxide
(30%, v/v; Merck, India) to terminate the reaction. The resulting
mixture was centrifuged at ∼3000g for 10 min,
and the pellet was washed with 10% hydrochloric acid solution to remove
the metal ions followed by repeated washing with distilled water to
get the solution having pH 7. Purified GO was finely dispersed in
distilled water and sonicated for 2–4 h to facilitate the synthesis
of nanoparticulates.
(GO) was synthesized using the modified Hummers method
followed by sonication.31 Briefly, graphite
flakes (Merck, USA), sodium nitrate, and potassium permanganate (SRL
Chemicals, Mumbai, India) were mixed at a ratio of 2:1:6 in the presence
of concentrated H2SO4 in an ice bath (at 0–5
°C). The reaction mixture was diluted by adding distilled water
under vigorous stirring followed by addition of hydrogen peroxide
(30%, v/v; Merck, India) to terminate the reaction. The resulting
mixture was centrifuged at ∼3000g for 10 min,
and the pellet was washed with 10% hydrochloric acid solution to remove
the metal ions followed by repeated washing with distilled water to
get the solution having pH 7. Purified GO was finely dispersed in
distilled water and sonicated for 2–4 h to facilitate the synthesis
of nanoparticulates.
3
Antioxidant and Anti-inflammatory Assays
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Acrylamide, ammonium per-sulfate, adenosine diphosphate, BSA, beta-mercaptoethanol, CDNB, cytochrome-C, glycine, thiobarbituric acid, Tris-HCl, xanthine, LPS (L4391), and xanthine oxidase were obtained from Sigma Chemicals, St. Louis, MO, USA. Dinitrophenyl hydrazine, EDTA, glutathione oxidized, glutathione reduced, malonaldehyde, NADPH, phosphoric acid, sulphanilamide, sulphosalicylic acid, sodium nitrate, t-butyl hydroperoxide were obtained from SRL Chemicals, Mumbai, India. The ELISA kit for measurement of COX-2 activity was purchased from Cayman Chemicals, Ann Arbor, MI, USA. Zerumbone (99.9% pure) was extracted from ginger as per the earlier procedure [15] .
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