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Kir sso genotyping test

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The KIR SSO Genotyping Test is a laboratory equipment product designed for the detection and identification of killer cell immunoglobulin-like receptor (KIR) genes. The test utilizes sequence-specific oligonucleotide (SSO) probes to determine the presence or absence of various KIR gene subtypes in a sample.

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Lab products found in correlation

Pcr sso reverse, by Thermo Fisher Scientific (1 mentions) Labscan 100, by Thermo Fisher Scientific (1 mentions) Dneasy blood tissue kit, by Qiagen (1 mentions) Luminex 200is, by DiaSorin (1 mentions) Qiaamp dna blood mini kit, by Qiagen (1 mentions)

4 protocols using kir sso genotyping test

1

HLA and KIR Genotyping for Transplant

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Donor and recipient HLA typing were performed by PCR-SSO reverse (One Lambda, Canoga Park, CA, USA). HLA-C1 and C2 groups were determined for the donors and recipients considering the HLA C typing obtained by PCR-SSO reverse (One Lambda). The presence or absence of Bw4 motif was determined for the donors and recipients, considering the HLA A and B typing obtained by PCR-SSO reverse (One Lambda).
Recipients were genotyped for the 14 KIR genes (2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 3DL1, 3DL2, 3DL3, 3DS1) and two pseudogenes (2DP1, 3DP1) by PCR-SSO reverse (KIR SSO Genotyping Test, One Lambda and Lifecodes KIR Genotyping, Immucor).
Koenig A., Chen C.C., Marçais A., Barba T., Mathias V., Sicard A., Rabeyrin M., Racapé M., Duong-Van-Huyen J.P., Bruneval P., Loupy A., Dussurgey S., Ducreux S., Meas-Yedid V., Olivo-Marin J.C., Paidassi H., Guillemain R., Taupin J.L., Callemeyn J., Morelon E., Nicoletti A., Charreau B., Dubois V., Naesens M., Walzer T., Defrance T, & Thaunat O. (2019). Missing self triggers NK cell-mediated chronic vascular rejection of solid organ transplants. Nature Communications, 10, 5350.
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2

Comprehensive KIR Genotyping Protocol

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KIR genotyping was available for 98 recipients. Genotyping of 16 KIR genes (2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 2DP1, 3DL1, 3DL2, 3DL3, 3DS1, and 3DP1) was performed by the KIR SSO genotyping test (One Lambda, Canoga Park, CA, USA), according to the manufacturer's instructions. The KIR SSO genotyping test applies Luminex technology to the reverse SSOP DNA typing. Three sets of specific primers for exons 3+4, 5, and 7-9 were used for DNA amplification. Each PCR product was biotinylated, which allows later detection using R-phycoerythrin-conjugated streptavidin. PCR products were denatured and hybridized to complementary DNA probes conjugated to fluorescently coded microspheres. After washing the beads, bound amplified DNA was tagged with R-Phycoerythrin-conjugated Streptavidin (SAPE), and a LABScan 100 (One Lambda) was used to measure the fluorescent intensity of each microsphere. Genotype classification was based on the patient's reaction pattern compared to patterns associated with published KIR gene sequences.
Lee H., Park K.H., Park H.S., Ryu J.H., Lim J., Kim Y., Na G.H., Kim D.G, & Oh E.J. (2016). Human Leukocyte Antigen-C Genotype and Killer Immunoglobulin-like Receptor-Ligand Matching in Korean Living Donor Liver Transplantation. Annals of Laboratory Medicine, 37(1), 45-52.
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3

KIR Genotyping and Ligand Identification

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DNA from all patients was extracted from thawed PBMC with the DNeasy Blood & Tissue Kit (Qiagen, catalog number: 69504) according to the manufacturer’s instructions. KIR genotyping was performed with the KIR SSO Genotyping Test (One Lambda, catalog number: RSSOKIR) and KIR-ligands were identified with the KIR HLA Ligand SSP typing kit (Olerup, catalog number: 104.201-12).
Strunz B., Hengst J., Deterding K., Manns M.P., Cornberg M., Ljunggren H.G., Wedemeyer H, & Björkström N.K. (2018). Chronic hepatitis C virus infection irreversibly impacts human natural killer cell repertoire diversity. Nature Communications, 9, 2275.
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4

KIR Genotyping of Mother-Infant Pairs

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The KIR genotyping method used in this study has been described previously [38 (link)]. Briefly, DNA was extracted from blood samples from 313 mother-infant pairs using the QIAamp DNA blood mini kit (Qiagen, Valencia, California, USA). KIR genotyping for 16 KIR genes was carried out using KIRSSO genotyping test (One Lambda Inc., Canoga Park, California, USA) based on the manufacturer instructions. The results were read on a Luminex 200 IS (Luminex Corp., Austin, Texas, USA). The presence of individual KIR genes was determined using HLA Fusion Beta software (One Lambda Inc., Canoga Park, California, USA). Positive control DNA samples with different profiles of KIR gene content from the International Histocompatibility Working Group (IHWG) were used in all experiments.
Omosun Y.O., Blackstock A.J., Williamson J., van Eijk A.M., Ayisi J., Otieno J., Lal R.B., ter Kuile F.O., Slutsker L, & Shi Y.P. (2018). Association of maternal KIR gene content polymorphisms with reduction in perinatal transmission of HIV-1. PLoS ONE, 13(1), e0191733.
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