4t1 mouse breast tumor cells

HEK 293T human embryonic kidney cells, MDA-MB231 human breast tumor cells, MCF-7 human breast tumor cells, 4T1 mouse breast tumor cells, and Panc-1 human pancreatic tumor cells were purchased from American Type Culture Collection (ATCC, Rockville, MD). MDA-MB231-luc and 4T1-luc that express luciferase were obtained from Perkin Elmer (Waltham, MA). All cells were grown in Dulbecco's modified Eagle's medium (DMEM, HyClone, South Logan, UT) except MCF-7 cells, which were grown in Roswell park memorial institute medium (RPMI, HyClone). All media were supplemented with 10% fetal bovine serum (FBS, ThermoFisher Scientific, Waltham, MA). Cells were maintained at 37 °C in a humidified atmosphere containing 5% CO₂.

4T1 mouse breast tumor cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum, 100 U/ml penicillin and 100 μg/ml streptomycin in a humidified incubator at 37°C with 5% CO₂.
BALB/c mice were purchased from the Experimental Animal Center of Nanjing Medical University (Nanjing, China) and maintained under pathogen-free conditions according to protocols that were approved by the Jiangsu Province Animal Care and Use Committee.