DLD-1 (CCL-221), HCT116 (CCL-247), HT29 (HTB-38), and SW620 (CCL-227) colorectal cancer cell lines were purchased from the American Type Culture Collection (ATCC) in 2015, 2016, and 2018. DLD-1 and SW620 cells were maintained in RPMI1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin, while HCT116 and HT29 in McCoy’s medium supplemented with 10% FBS and 1% penicillin/streptomycin. The DLD-1/pGL4.18hKLF5p cell line was maintained in RPMI1640 with 10% FBS and 1% penicillin/streptomycin supplemented with 800 μg/mL of geneticin (21 (link)). The cell lines were passaged for three months while used for experiments. We tested all cell lines for Mycoplasma contamination upon thawing and routinely performed morphology checks on all tested cell lines. Furthermore, each experiment had appropriate controls to assure the behavior of tested cell lines and was performed with an appropriate number of biological replicates. The previously-described lead compound ML264 and subsequent new leads SR15006 and SR18662 were synthesized at The Scripps Research Institute in the laboratory of Dr. Thomas Bannister. For in vitro experiments the lead compounds were dissolved in dimethyl sulfoxide (DMSO, Fisher Scientific).
Sw620 ccl 227
Manufactured by American Type Culture Collection
Sourced in United States
The SW620 (CCL-227) is a human colorectal adenocarcinoma cell line. It is used for research purposes in cell biology, cancer research, and drug development.
Automatically generated - may contain errors
Lab products found in correlation
Ht 29 htb 38, by American Type Culture Collection (2 mentions)
Hct116 ccl 247, by American Type Culture Collection (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Sw480 ccl 228, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
293 cells, by Thermo Fisher Scientific (1 mentions)
Penicillin g, by Thermo Fisher Scientific (1 mentions)
Lovo ccl 229, by American Type Culture Collection (1 mentions)
Glutamine, by Euroclone (1 mentions)
Chloroquine clq, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
7 protocols using sw620 ccl 227
1
Colorectal Cancer Cell Line Maintenance and Compound Testing
2
Glycyrrhizic Acid Treatment of Colorectal Cancer Cell Lines
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SW620 (CCL-227) and HT29 (HTB-38) cell lines were purchased from the American Type Culture Collection (ATCC). They were cultured in McCoy’s 5A medium (12330031, Gbico) supplemented with 10% fetal bovine serum (16140071, Gbico) at 37°C and 5% CO2. Different concentrations of GA (1295888, Sigma) were directly added to the medium to perform treatments (GA, Figure 1 ).
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Molecular formula of glycyrrhizic acid.
3
Culturing Colon Cancer Cell Lines
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Human SW620 (CCL-227™) and SW480 (CCL-228™) colon carcinoma cells were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). In addition, HCT116 and HT29 colon cancer cell lines, as well as human normal colon epithelium FHC cell line, were purchased from Shanghai Bioleaf Biotech Co., Ltd. (Shanghai, China). The aforementioned cells were cultured in RPMI 1640 medium (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) containing 10% fetal bovine serum (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in a 5% CO2 atmosphere at 37°C.
4
Cell Culture Protocol for Colorectal Cancer
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Human normal colorectal mucosa cells (FHC; CRL-1831) and CRC cell lines [SW620 (CCL-227), SW480 (CCL-228), HT29 (HTB-38), HCT116 (CCL-247), LOVO (CCL-229), DLD-1 (CCL-221)] were obtained from American Type Culture Collection. All cells were authenticated using STR profiling. The cells were maintained in high-glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics (all Gibco; Thermo Fisher Scientific, Inc.) at 37˚C in a humidified incubator containing 5% CO2.
5
Culturing Human Colon Cancer Cell Lines
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Five human colon adenocarcinoma cell lines, HCT116 (CCL247), SW480 (CCL-228), SW620 (CCL-227), LOVO (CCL-229) and HT29 (HTB-38) were obtained from American Type Culture Collection (Manassas, VA, USA). The cells were cultured overnight, prior to transfection, in Dulbecco's modified Eagle's medium (DMEM) or L15 medium (each, Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% streptomycin in a humidified environment at 5% CO2 and 37°C. 293 cells (Gibco; Thermo Fisher Scientific, Inc.) were grown in DMEM medium, supplemented with 10% FBS, 100 U/ml penicillin G and 100 µg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.). All cells were maintained at 37°C in a humidified 5% CO2 atmosphere.
6
Colorectal Cancer Cell Lines: Treatments
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Human colorectal cancer HCT116 (CCL-247™) and SW620 (CCL-227™) cell lines, with different genetic background (Table 1 ) and tumor site origins, were purchased from the American Type Culture Collection (ATCC). HCT116 and SW620 cell lines were maintained in standard culture conditions (37 °C, 95 v/v% air: 5 v/v% CO2) in Dulbecco’s Modified Eagle Medium (DMEM, cod. D5671; Sigma-Aldrich, St. Louis, MO, USA) and RPMI-1640 Medium (RPMI, cod. R8758; Sigma-Aldrich, St. Louis, MO, USA), respectively, supplemented with 10% heated-inactivated fetal bovine serum (FBS, cod. ECS0180L; Euroclone, Milano, Italy), 1% glutamine (cod. G7513; Sigma Aldrich, St. Louis, MO, USA) and 1% penicillin/streptomycin (cod. P0781; Sigma-Aldrich, St. Louis, MO, USA). Treatments included 50 ng/mL interleukin-6 (IL-6, cod. 11340066; Immunotools, Friesoythe, Germany), 1 mM and 2 mM sodium butyrate (NaB, cod. B5887; Sigma-Aldrich, St. Louis, MO, USA), and 30 μM chloroquine (ClQ, cod. C-6628; Sigma-Aldrich, St. Louis, MO, USA). All three reagents were dissolved in sterile water.
7
Cell Culture of Colorectal Cancer Lines
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Human colorectal cancer cell lines SW620 (CCL-227) and SW480 (CCL-228) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and were cultivated in Leibovitz’s L-15 Medium (ATCC) contained 10% fetal bovine serum (FBS, Gibco). LOVO cells (TCHu 82) were purchased from the Shanghai Cell Bank (Chinese Academy of Sciences, Shanghai, China) and were cultivated in dulbecco’s modified eagle medium (DMEM, Gibco) which was rich in 10% FBS. All cells were placed in a humidified incubator balanced with 5% CO2 at 37 °C. All cell lines were authenticated by short tandem repeats (STR) profiling (done by Cobioer, Nanjing, China). None of the cells were contaminated with mycoplasma.
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