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Dykddddk clone l5

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The DYKDDDDK (clone L5) is a mouse monoclonal antibody that specifically recognizes the DYKDDDDK epitope tag. It can be used for the detection and purification of proteins tagged with the DYKDDDDK sequence.

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Lab products found in correlation

Pe conjugated streptavidin, by Jackson ImmunoResearch (2 mentions) Macsquant vyb cytometer, by Miltenyi Biotec (2 mentions) Cd69 clone fn50, by BioLegend (2 mentions)

2 protocols using dykddddk clone l5

1

Flow Cytometry Analysis of T-cell Epitopes

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Unless otherwise specified, flow cytometry in this study was performed with a MACSQuant VYB cytometer (Miltenyi Biotec). T-cells were assessed for surface presentation of epitopes using fluorescently labeled monoclonal antibodies for CD69 (clone FN50, BioLegend), or DYKDDDDK (clone L5, BioLegend; or #130–101-571, Miltenyi Biotec). EGFRt expression was measured with Erbitux (Bristol-Myers Squibb) biotinylated in-house (EZ-link Sulfo-NHS-Biotin, Pierce) followed by PE-conjugated streptavidin (Jackson ImmunoResearch). Flow data were analyzed and gated in FlowJo (TreeStar) (see Supplementary Fig. 8 for an example of gating strategy). Unless otherwise noted, data shown are drawn from biological triplicates (i.e., three distinct samples).
Chang Z.L., Lorenzini M.H., Chen X., Tran U., Bangayan N.J, & Chen Y.Y. (2018). Rewiring T-cell responses to soluble factors with chimeric antigen receptors. Nature chemical biology, 14(3), 317-324.
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2

Flow Cytometry Analysis of T-cell Epitopes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless otherwise specified, flow cytometry in this study was performed with a MACSQuant VYB cytometer (Miltenyi Biotec). T-cells were assessed for surface presentation of epitopes using fluorescently labeled monoclonal antibodies for CD69 (clone FN50, BioLegend), or DYKDDDDK (clone L5, BioLegend; or #130–101-571, Miltenyi Biotec). EGFRt expression was measured with Erbitux (Bristol-Myers Squibb) biotinylated in-house (EZ-link Sulfo-NHS-Biotin, Pierce) followed by PE-conjugated streptavidin (Jackson ImmunoResearch). Flow data were analyzed and gated in FlowJo (TreeStar) (see Supplementary Fig. 8 for an example of gating strategy). Unless otherwise noted, data shown are drawn from biological triplicates (i.e., three distinct samples).
Chang Z.L., Lorenzini M.H., Chen X., Tran U., Bangayan N.J, & Chen Y.Y. (2018). Rewiring T-cell responses to soluble factors with chimeric antigen receptors. Nature chemical biology, 14(3), 317-324.
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