Human bladder cancer cell lines HT1376, T24, UMUC3, RT112 and TCCSUP were originally from ATCC and were kindly provided by Drs. Yuesheng Zhang and Candace Johnson, Roswell Park Comprehensive Cancer Center. Dr. Johnson’s lab also provided human bladder cancer cell line 253 J B-V for this study. Human colorectal cancer (CRC) cell lines SNU-C2B and HCT117 were purchased from ATCC. Cells were grown under standard culture conditions at 37 °C in a humidified atmosphere containing 5% CO2. Cells were cultured in DMEM supplemented with 10% FBS, 100 unit/mL of penicillin and 100 μg/mL of streptomycin. Sub-confluent cells were treated with different concentrations of FL118 or with an equivalent amount of solvent DMSO according to the experiments.
Snu c2b
Manufactured by American Type Culture Collection
The SNU-C2B is a laboratory instrument used for the maintenance and propagation of cell cultures. It provides a controlled environment for the growth and expansion of various cell types, including those derived from human, animal, or microbial sources. The SNU-C2B offers precise temperature, humidity, and gas composition regulation to support optimal cell culture conditions.
Automatically generated - may contain errors
2 protocols using snu c2b
1
Bladder and Colorectal Cancer Cell Lines
2
Immunofluorescence Assay for Colon Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Malignant human colon cancer cell lines HCT116 wt (having wt p53), HCT116 p53-/-and p53 mutant cell lines HRA19 (Agr273His) and SNU-C2B (Arg273His) from ATCC. HCT116wt cell line was cultured in RPMI-1640 supplemented with 10%FBS and the remaining cell lines were cultured using DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10%FBS 10 .
30,000 cells from each of the cell lines were plated in 35mm cover slip stuck petridishes and allowed to grow for 24hrs and then taken for Immunofluorescence.
30,000 cells from each of the cell lines were plated in 35mm cover slip stuck petridishes and allowed to grow for 24hrs and then taken for Immunofluorescence.
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