Log In Sign up
The largest database of trusted experimental protocols

Sc 8417

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States, United Kingdom

Sc-8417 is a laboratory instrument designed for protein and nucleic acid analysis. It is capable of performing electrophoresis and blotting techniques, which are essential for separating and detecting biomolecules. The core function of Sc-8417 is to provide a platform for conducting these analytical procedures in a research or diagnostic setting.

Automatically generated - may contain errors

Lab products found in correlation

Mab350, by R&D Systems (2 mentions) Goat anti mouse p0447, by Agilent Technologies (2 mentions) Isotype control antibody, by R&D Systems (1 mentions) Sc 432, by Santa Cruz Biotechnology (1 mentions)

3 protocols using sc 8417

1

Immunohistochemical Staining of Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen human tissue sections (synovial tissues, tumour tissues, or normal healthy tissues) were cut (5 µm) and mounted on Star Frost adhesive glass slides (Knittelgläser, Braunschweig, Germany). Sections were fixed with acetone and endogenous peroxidase activity was blocked with 0.3% H2O2 in 0.1% sodium azide in PBS. Sections were stained with monoclonal primary antibodies against NIK (sc-8417; Santa Cruz Biotechnology, Santa Cruz, CA, USA), p52 (#4882; Cell Signaling Technology, Danvers, MA, USA), and CXCL12 (MAB350; R&D Systems, Abingdon, UK), and secondary antibodies goat anti-mouse (p0447; DAKO, Glostrup, Denmark) or swine anti-rabbit (p0399; DAKO), and streptavidin-labelled with horseradish peroxidase. Biotinylated tyramine was used for amplification, as previously described 33 (link). As negative controls, sections were incubated with isotype control antibodies (all R&D Systems).
Noort A.R., van Zoest K.P., Weijers E.M., Koolwijk P., Maracle C.X., Novack D.V., Siemerink M.J., Schlingemann R.O., Tak P.P, & Tas S.W. (2014). NF-κB-inducing kinase is a key regulator of inflammation-induced and tumour-associated angiogenesis. The Journal of Pathology, 234(3), 375-385.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Tissue Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen human tissue sections (synovial tissues, tumor tissues, or normal healthy
tissues) were cut (5 μm) and mounted on Star Frost adhesive glass slides
(Knittelgläser, Braunschweig, Germany). Sections were fixed with acetone and
endogenous peroxidase activity was blocked with 0.3% H2O2 in 0.1%
sodium azide in PBS. Sections were stained with monoclonal primary antibodies against NIK
(sc-8417, Santa Cruz Biotechnology, Santa Cruz, CA), p52 (#4882, Cell Signaling
technology, Danvers, MA) and CXCL12 (MAB350, R&D Systems, Abingdon, United Kingdom)
and secondary antibodies goat-anti-mouse (p0447, DAKO, Glostrup, Denmark) or
swine-anti-rabbit (p0399, DAKO) and streptavidin labeled with horseradish peroxidase.
Biotinylated tyramine was used for amplification, as previously described [33 ]. As negative controls, sections were incubated with
isotype control antibodies (all R&D Systems).
Noort A.R., van Zoest K.P., Weijers E.M., Koolwijk P., Maracle C.X., Novack D.V., Siemerink M.J., Schlingemann R.O., Tak P.P, & Tas S.W. (2014). NF-κB-inducing kinase is a key regulator of inflammation-induced and tumour-associated angiogenesis. The Journal of Pathology, 234(3), 375-385.
+ Open protocol
+ Expand
3

Immunohistochemical Analysis of TLS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen ST biopsy samples were cut in 5-mm sections and mounted on Star Frost adhesive glass slides (Knittelgläser, Braunschweig, Germany). Sections were fixed with acetone. Endogenous peroxidase activity was blocked with 0.3% H 2 O 2 in 0.1% sodium azide in phosphate-buffered saline for 20 minutes. Sections were stained with mouse monoclonal primary antibodies against NIK (sc-8417, Santa Cruz Biotechnology, Santa Cruz, CA), FDC-M1 (14-9968-80, eBioscience, San Diego, CA), rabbit polyclonal primary antibodies against PDGFRb (sc-432; Santa Cruz Biotechnology), and the secondary antibodies goateanti-mouse (p0447; Dako, Glostrup, Denmark) or swineeanti-rabbit (p0399; Dako) and streptavidin labeled with horseradish peroxidase. Biotinylated tyramide was used for amplification, as previously described. 30 As a negative control, isotype-matched immunoglobulins were applied to the sections instead of the primary antibody. Expression was scored semiquantitatively on a scale of 0 to 4 by two blinded researchers independently (A.R.N. and K.P.M.v.Z.). Semiquantitative scoring was used because digital image analysis is less suitable for comparing tissue with TLS versus tissue without TLS because information on the location of the cells in the tissue is lost.
Noort A.R., van Zoest K.P., van Baarsen L.G., Maracle C.X., Helder B., Papazian N., Romera-Hernandez M., Tak P.P., Cupedo T, & Tas S.W. (2015). Tertiary Lymphoid Structures in Rheumatoid Arthritis: NF-κB-Inducing Kinase-Positive Endothelial Cells as Central Players. The American journal of pathology, 185(7).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!