MB‐3 (M2449), complete Freund's adjuvant (CFA) (F5881), and LPS (E.coli 0111:B4) were purchased from Sigma–Aldrich. Chicken Type II Collagen (20088) was from Chondrex. ChIP‐grade protein G magnetic beads (9006) and cell lysis buffer (9803) were from Cell Signaling Technology (CST). Nigericin (tlrl‐nig), ATP (tlrl‐atpl), and MSU (tlrl‐msu) were from InvivoGen. Mouse IL‐1β, TNF‐α, and IL‐6 ELISA kits were from R&D Systems. Antibodies against KAT2A (3305), IL‐1β (12242), NRF2 (12721), IRG1 (19857), Acetyl‐Histone H3 Lys9 (9649), and β‐Actin (3700) were from CST. Antibodies against NLRP3 (AG‐20B‐0014B), caspase 1 (AG‐20B‐0042B), and ASC (AG‐25B‐0006PF) were from AdipoGen Life Sciences. Antibodies against CD45‐BV605 (2D1), CD4‐BV421 (GK1.5), CD8a‐BV510 (53‐6.7), CD11b‐BV510 (M1/70), F4/80‐FITC (BM8), CD44‐APC (IM7), CD62L‐PE (MEL‐14), PD‐1‐APC (29F.1A12), CXCR5‐PE (J252D4), IFN‐γ‐APC (XMG1.2), and IL‐17a‐PE (TC11‐18H10.1) were from Biolegend. Antibody against FoxP3‐PE (FJK‐16s) was obtained from eBioscience.
Msu tlrl msu
Manufactured by InvivoGen
The MSU (tlrl-msu) is a lab equipment product offered by InvivoGen. It is a magnetic separation unit designed to facilitate the isolation and purification of biomolecules and cells from complex samples. The core function of the MSU is to provide a simple and efficient method for magnetic separation, enabling users to separate target analytes from their samples.
Automatically generated - may contain errors
Lab products found in correlation
Lps e coli 0111 b4, by Merck Group (1 mentions)
Chip grade protein g magnetic beads 9006, by Cell Signaling Technology (1 mentions)
Cell lysis buffer 9803, by Cell Signaling Technology (1 mentions)
Lps l2880, by Merck Group (1 mentions)
Atp tlrl atpl, by InvivoGen (1 mentions)
Mouse il 1β elisa kit, by Dakewe (1 mentions)
Human il 1β elisa kit, by Dakewe (1 mentions)
Perfectstart green qpcr supermix, by Transgene (1 mentions)
Nucleozol, by Macherey-Nagel (1 mentions)
Mouse il 6 elisa kit, by Dakewe (1 mentions)
Mouse tnf α elisa kit, by Dakewe (1 mentions)
2 protocols using msu tlrl msu
1
Inflammasome Activation and Cytokine Profiling
2
Inflammatory Cytokine Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco’s modified Eagle medium (DMEM, high glucose, 11965092), fetal bovine serum (FBS, 10099141), Lipofectamine™ 2000 (11668019), Pierce™ BCA Protein Assay Kit (23225), and Thermo Scientific RevertAid Mix (M16325) were purchased from ThermoFisher Scientific (Waltham, MA, USA). Protease inhibitor cOmplete™ Cocktail (4693116001) was purchased from Roche (Basel, Switzerland). PerfectStart™ Green qPCR SuperMix (AQ601) was purchased from TransGen Biotech (Beijing, China). NucleoZOL (740404) was purchased from MACHEREY-NAGEL (MN, Düren, Deutschland). Mouse IL-1β ELISA kit (1210122), human IL-1β ELISA kit (1110122), mouse IL-6 ELISA kit (1210602), and mouse TNF-α ELISA kit (1217202) were purchased from DAKEWE (Shenzhen, China). LPS (L2880) was purchased from Sigma (Missouri, USA). ATP (tlrl-atpl) and MSU (tlrl-msu) were purchased from In vivoGen.
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