Anti trail r2 djr2 4

Manufactured by BioLegend

Anti-TRAIL-R2 (DJR2-4) is a monoclonal antibody that binds to TRAIL-R2 (also known as DR5), a receptor for the apoptosis-inducing ligand TRAIL. This antibody can be used for the detection and analysis of TRAIL-R2 expression on cells.

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Lab products found in correlation

Anti trail r1 djr1, by BioLegend (2 mentions) Mopc 21, by BioLegend (1 mentions) Anti granzyme b gb11, by BD (1 mentions)

Close spelling variants of this product

Anti-TRAIL (3 citations) TRAIL-R2 (2 citations)

2 protocols using anti trail r2 djr2 4

TRAIL Receptor Surface Expression

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For the TRAIL receptor stain we used monoclonal anti-TRAIL-R1 (DJR1) and anti-TRAIL-R2 (DJR2-4) antibodies (1 μg/10⁶ cells; BioLegend, San Diego, CA) that were conjugated to Phycoerythrin (PE). The isotype control antibody (MOPC-21) (1 μg/10⁶ cells) was also purchased from BioLegend. The surface expression of TRAIL receptors was measured by incubating cells with the PE-conjugated mouse anti-human TRAIL-R1 and mouse anti-human TRAIL-R2 antibodies as described previously [41 (link)].

Mohr A., Yu R, & Zwacka R.M. (2015). TRAIL-receptor preferences in pancreatic cancer cells revisited: Both TRAIL-R1 and TRAIL-R2 have a licence to kill. BMC Cancer, 15, 494.

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Immunophenotyping of NK Cells and Leukemic Blasts

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Peripheral whole blood (3 ml) was obtained at diagnosis for immunophenotyping by flow cytometry of NK cells and leukemic blasts. The following antibodies were used to enumerate NK cells and their surface expression: anti-KIR2DL1 (143211), anti-NTB-A (292811), anti-ULBP1 (170818), anti-ULBP2 (165903), anti-ULBP3 (166510), anti-MICA (159227), and anti-MICB (236511) were obtained from R&D Systems; anti-KIR3DL1 (DX9), anti-KIR2DL2/3 (CH-L), anti-CD11a (HI111), anti-NKG2D (1D11), anti-TRAIL (RIK-2), anti-DNAM (DX11), anti-2B4 (2–69), and anti-granzyme B (GB11) from BD Bioscience; anti-NKG2A (Z199), anti-NKp30 (Z25), anti-NKp44 (Z231), anti-NKp46 (BAB281), and anti-FAS (CIB2) from Beckman Coulter; and anti-FASL (14C2) from AbD Serotec. Blasts obtained at diagnosis were evaluated by anti-NTB-A (292811) from R&D Systems; anti-ICAM (84H10), anti-FAS (CIB2) and anti-CD48 (J4-57) from Beckman Coulter; anti-Nectin (R2.525) from BD Bioscience, anti-TRAIL-R1 (DJR1) and anti-TRAIL-R2 (DJR2-4) from Biolegend; anti-PVR (TX21) from MBL International; anti-HLA-ABCEFG (W6/32) from Dako; and anti-PI-9 (7D8) from AbD Serotec.

Sullivan E.M., Jeha S., Kang G., Cheng C., Rooney B., Holladay M., Bari R., Schell S., Tuggle M., Pui C.H, & Leung W. (2014). NK Cell Genotype and Phenotype at Diagnosis of Acute Lymphoblastic Leukemia Correlate to Post-induction Residual Disease. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(23), 5986-5994.

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