TRIzol was used for total RNA extraction according to the manufacturer's protocol (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA). Small RNAs (<200 nt) were separated from the total RNA using mirVana miRNA purification columns (Ambion; Thermo Fisher Scientific, Inc.) for microarray analysis and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis according to the manufacturer's protocol. The quality and quantity of each RNA preparation were determined using a Nanodrop ND-1000 spectrophotometer (Agilent Technologies, Inc., Santa Clara, CA, USA).
Mirvana mirna purification columns
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MirVana miRNA purification columns are a tool designed for the isolation and purification of microRNA (miRNA) from a variety of sample types, including cells, tissues, and body fluids. The columns utilize proprietary technology to selectively capture and recover miRNA molecules, enabling researchers to study these important regulatory RNAs.
Automatically generated - may contain errors
2 protocols using mirvana mirna purification columns
1
Small RNA Extraction and Analysis
2
Gastric Cancer Tissue RNA Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In total, 34 gastric cancer tissues and paired adjacent normal
gastric tissues were homogenized with Tissue Lyser2 (Qiagen, Germantown, MD, USA). TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) was used for total RNA extraction. Low-molecular-weight RNAs (<200 nucleotides [nt]) were separated from total RNA using mirVana miRNA purification columns (Ambion, Austin, TX, USA) for microarray analysis and quantitative polymerase chain reaction (qPCR). The quality and quantity of each RNA sample were determined using a NanoDrop ND-1000 spectrophotometer (Agilent Technologies UK, Ltd., West Lothian, UK).
gastric tissues were homogenized with Tissue Lyser2 (Qiagen, Germantown, MD, USA). TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) was used for total RNA extraction. Low-molecular-weight RNAs (<200 nucleotides [nt]) were separated from total RNA using mirVana miRNA purification columns (Ambion, Austin, TX, USA) for microarray analysis and quantitative polymerase chain reaction (qPCR). The quality and quantity of each RNA sample were determined using a NanoDrop ND-1000 spectrophotometer (Agilent Technologies UK, Ltd., West Lothian, UK).
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