The fixed specimens were imaged and detected employing a GE eXplore Locus SP Micro-CT (GE Healthcare, Chicago, IL, USA) to visualize the new bone formed around the implants. The parameters were set at 80 μA current and 80 kV voltage, with an exposure time of 3,000 ms. The 3D images were reconstructed using GEHC MicroView software (GE Health-care, London, UK) after scanning. To obtain the parameters of bone mineral density and bone volume fraction (bone volume/total volume) of the newly formed bone around the implant, a region with a radius of 0.5 mm from the implant surface within the bone marrow cavity was chosen for analysis.
Ge explore locus sp micro ct
Manufactured by GE Healthcare
Sourced in United States
The GE eXplore Locus SP Micro-CT is a laboratory imaging system used for high-resolution, three-dimensional imaging of small samples. It employs X-ray technology to capture detailed images of the internal structure and composition of objects.
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6 protocols using ge explore locus sp micro ct
1
Micro-CT Analysis of Bone Formation
2
Micro-CT Analysis of Damaged Bone
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Damaged bone around the particles was detected using the GE Explore Locus SP Micro-CT (GE Healthcare, Chicago, IL, USA), and 3D images were reconstructed. The bone volume fraction (bone volume/total volume) and trabecular pattern factor were determined using SkyScan DataViewer and CTAn software (Bruker, Karlsruhe, Germany). The region of interest was from the growth plate to the trabecular bone under the growth plate with a thickness of 200 micro-CT layers [2 (link)].
3
Micro-CT Analysis of Bone Regeneration
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The presence of newly formed bone around the implants was detected by Micro-CT (GE explore Locus SP Micro-CT, United States). The parameters of scanning were set at 80 kV with an exposure time of 3,000 ms and a resolution of 15 µm. Three-dimensional images were reconstructed using NRecon software (SkyScan, United States) and a CTvol program (SkyScan). The bone volume fraction (bone volume/total volume, BV/TV) and trabecular bone thickness (Tb.Th) were determined for newly grown bone tissues using DataViewer software (SkyScan) and a CTAn program (SkyScan).
4
Micro-CT Analysis of Specimen Morphology
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The specimens were scanned using a micro-CT system (GEe Xplore Locus SP Micro-CT: GE Healthcare, Milwaukee, WI, USA), and the scan resolution was 10 μm. 3D reconstruction and morphometric parameters were analyzed using the CTAn software (Bruker Corporation, Kontich, Belgium).
5
Femur Bone Density and Microarchitecture in Mice
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To determine the bone mineral density (BMD) and microarchitecture of femur trabeculae in mice, the left femur was scanned using a micro-CT scanner (GE eXplore Locus SP Micro-CT, GE Healthcare, Barrington, IL, USA). BMC, BS/BV, BV/TV, Tb.Th, Tb.N, Tb.Sp, SMI, and connectivity density were calculated from the region of interest (ROI). For cortical bone analysis, data, including total area (Tt.Ar), marrow area (Ma.Ar), and cortical area fraction (Ct.Ar/Tt.Ar), were taken from a 2-mm-long round region of the mid-diaphysis femur. All of the micro-CT data were calculated using MicroView v2.1.1 software and Advanced Bone Analysis application (GE Healthcare, IL, USA).
6
Micro-CT Bone Volume Analysis
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The specimens were examined using a micro-CT scanning system (GEe Xplore Locus SP Micro-CT; GE Healthcare, Milwaukee, WI, USA), which was operated at 45 kV and 80 mA with a resolution of 20 μm. 3D images of the specimens were reconstructed from 2D slices and the morpho-metric parameter of bone volume per total volume (BV/TV) was analyzed using the CTAn software (Bruker Corporation, Kontich, Belgium).
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