Procartaplex nhp cytokine chemokine panel 30plex

Manufactured by Thermo Fisher Scientific

The ProcartaPlex NHP Cytokine & Chemokine Panel 30plex is a multiplexed assay designed to measure the levels of 30 different cytokines and chemokines in non-human primate (NHP) samples. This panel provides a comprehensive profile of immune function and inflammatory status in NHP research models.

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Lab products found in correlation

Magpix, by DiaSorin (2 mentions) Belysa immunoassay curve fitting software, by Merck Group (1 mentions)

2 protocols using procartaplex nhp cytokine chemokine panel 30plex

Plasma Cytokine and Chemokine Profiling

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Plasma cytokine and chemokine analyses at baseline and 24 h and 14 days after the first dose were performed using the ProcartaPlex NHP Cytokine & Chemokine Panel 30plex (Thermo Fisher Scientific) according to the manufacturer’s instructions. Samples were analyzed using a MagPix (Luminex) instrument, and the data were analyzed with Belysa Immunoassay Curve Fitting software (Millipore). Standard curves were generated using 5-parameter logistic (5-PL) curve fit.

Lenart K., Hellgren F., Ols S., Yan X., Cagigi A., Cerveira R.A., Winge I., Hanczak J., Mueller S.O., Jasny E., Schwendt K., Rauch S., Petsch B, & Loré K. (2022). A third dose of the unmodified COVID-19 mRNA vaccine CVnCoV enhances quality and quantity of immune responses. Molecular Therapy. Methods & Clinical Development, 27, 309-323.

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Multiplex Cytokine Profiling in NHPs

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After the first immunization, plasma cytokines and chemokines were analyzed using the ProcartaPlex NHP Cytokine & Chemokine Panel 30plex (Thermo Fisher Scientific) according to the manufacturer’s instructions at the Affinity Proteomics core facility, SciLifeLab, Stockholm, Sweden. Samples collected at baseline, 24 h, and 14 days after the immunization were analyzed using a MagPix (Luminex) instrument, and the data were analyzed with Belysa Immunoassay Curve Fitting software (Millipore). Standard curves were generated using 5-parameter logistic curve fit.

Lenart K., Arcoverde Cerveira R., Hellgren F., Ols S., Sheward D.J., Kim C., Cagigi A., Gagne M., Davis B., Germosen D., Roy V., Alter G., Letscher H., Van Wassenhove J., Gros W., Gallouët A.S., Le Grand R., Kleanthous H., Guebre-Xabier M., Murrell B., Patel N., Glenn G., Smith G, & Loré K. (2024). Three immunizations with Novavax’s protein vaccines increase antibody breadth and provide durable protection from SARS-CoV-2. NPJ Vaccines, 9, 17.

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