Gastric cancer cell lines, AGS and NCI-N87 were purchased from ATCC, USA. IM95, NUGC-3 and NUGC-4 were purchased from Health Science Research Resources Bank, Japan. MKN1, MKN45, TMK1 and YCC10 were obtained from DUKE NUS, Singapore. HGC-27 was purchased from Public Health England, UK. All cell lines except YCC10 cell lines were cultured in RPMI 1640 medium (Gibco; Grand Island, NY) containing 10% heat inactivated fetal bovine serum (Gibco; Grand Island, NY) and 1% penicillin/streptomycin (Gibco; Grand Island, NY). YCC10 cell lines was cultured in MEM supplemented with 20% heat inactivated fetal bovine serum (Gibco; Grand Island, NY), 1% penicillin/streptomycin (Gibco; Grand Island, NY) and 1% sodium pyruvate (Gibco; Grand Island, NY). All cell lines are maintained at 37 °C in humidified atmosphere containing 5% CO2.
Nugc 4
Manufactured by Health Science Research Resources Bank
Sourced in Japan
NUGC-4 is a laboratory equipment designed for cell culture applications. It functions as an incubator, providing a controlled environment for the growth and maintenance of cell lines. The NUGC-4 maintains stable temperature, humidity, and atmospheric conditions to support the in vitro cultivation of various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Nci n87, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Mkn45, by Health Science Research Resources Bank (1 mentions)
Mkn74, by Health Science Research Resources Bank (1 mentions)
Az521, by RIKEN BioResource Center (1 mentions)
Ke 97, by RIKEN BioResource Center (1 mentions)
Kato 3, by Health Science Research Resources Bank (1 mentions)
Ecc12, by RIKEN BioResource Center (1 mentions)
Snu 5, by American Type Culture Collection (1 mentions)
2 protocols using nugc 4
1
Gastric Cancer Cell Line Culturing
2
Gastric Cancer Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human gastric cancer cell lines used, that is, HSC-39, HSC-43, HSC-59, HSC-60, HSC-64, HSC-44PE, 58As9, 58As1, 44As3 and 44As3Luc, have been described previously.31 (link)–34 (link) MKN1, MKN7, MKN74, NUGC-4, KATO-III, MKN45 and IM95 were obtained from the Health Science Research Resources Bank. AGS, NCI-N87 and SNU-5 were obtained from the American Type Culture Collection (ATCC). GCIY, ECC12, AZ521 and KE-97 were provided by the RIKEN Bio-Resource Center through the National Bio-Resource Project of the MEXT, Japan. These cells were maintained in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS, 10 units/mL of penicillin and 10 μg/mL of streptomycin at 37°C in a humidified atmosphere containing 5% CO2.
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