Bacteria were purchased from ATCC and/or isolated in our laboratory and were cultured in specified liquid media. Samples were collected at baseline and again at 18 hours after incubation at 37°C. Lactococcus lactis Subsp. cremoris (LC, ATCC 19257), Lactobacillus rhamnosus GG (LGG, ATCC 53103), Lactobacillus paracasei (HA274, HA196), and Lactobacillus rhamnosus (HA-114, HA-111, R0011) were grown in MRS broth (Oxoid, CM0359). Bacillus cereus, Listeria monocytogenes (LM), and Streptococcus salivarius (ATCC13419) were grown in brain heart infusion (BHI) media. E. coli (K12) and Salmonella typhimurium (SL1344) were grown in Luria Broth (LB). Bifidobacterium longum (BL, ATCC15707) was grown anaerobically using Oxyrase for Broth (Sigma, SAE0013) in reinforced clostridial media.
Oxyrase for broth
Manufactured by Merck Group
Sourced in United States
Oxyrase for Broth is a product manufactured by Merck Group. It is a laboratory reagent used to create anaerobic conditions in liquid culture media, such as broths, for the growth of anaerobic microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using oxyrase for broth
1
Culturing Diverse Bacterial Strains
2
Culturing Diverse Bacterial Strains
3
Cultivation of Gut Commensal Bacteria
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De Man, Rogosa and Sharpe (MRS), Brain Heart Infusion (BHI) and Fastidious Anaerobe Broth (FAB) media were obtained from Criterion (Santa Maria, CA, USA). Mucin from porcine stomach and yeast extract were purchased from Sigma (St. Louis, MO, USA). Twelve (12) commensal bacterial strains were purchased from the American Type Culture Collection (Table S1 ). The intestinal strains were selected in a way to represent the main abundant phyla in the human gut, notably Bacteroidetes, Firmicutes, Actinobacteria, Proteobacteria and Verrucomicrobia. All strains were cultured in their recommended media (Table S1 ). Frozen stocks of strains were maintained at − 80 °C until use. Bacteria strains were grown in their appropriate media a least three times at 37 °C before each experiment to obtain a robustly and uniformly growing culture. The enzyme Oxyrase for broth purchased from Sigma Aldrich (St. Louis, MO, USA) was added to culture media at 1% to promote the growth of the anaerobic strains.
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