For chondrogenesis in hMSCs, 250,000 MSCs were centrifuged at 500 g, 7 min in 15 ml tubes to form pellets at high-density culture. Chondrogenesis was induced for 18 days with MEM medium supplemented with 50 μg/ml L-ascorbic acid-2-phosphate (Sigma-Aldrich.), 1% ITS+1 (BD Bioscience), 10−7 M dexamethasone (Sigma-Aldrich), and 10 ng/ml TGFb3 (R&D Systems, Wiesbaden, Germany). The aggregated cells were cultured in tubes with 0.5-1 ml medium/pellet at 37°C in a humidified atmosphere containing 95% air and 5% CO2. The medium was replaced every other day for 18 days [25 (link)].
Its 1
Manufactured by BD
Sourced in Germany, United States
The ITS+1 is a laboratory equipment product developed by BD. It is designed to perform specific functions in a laboratory setting. The core function of the ITS+1 is to provide a controlled environment for various laboratory processes and procedures. The detailed specifications and intended use of the ITS+1 are not available for an unbiased and factual description.
Automatically generated - may contain errors
Lab products found in correlation
Dexamethasone (dex), by Merck Group (2 mentions)
Tgfb3, by R&D Systems (1 mentions)
L ascorbic acid 2 phosphate, by Merck Group (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
L proline, by Merck Group (1 mentions)
Gentamicin, by Merck Group (1 mentions)
Tgf β1, by Merck Group (1 mentions)
2 protocols using its 1
1
Chondrogenic Differentiation of hMSCs
2
Chondrogenic Differentiation of HAC and BMSC
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Following expansion or 48 h after the second silencing treatment, HAC pellets and BMSC pellets containing 2x10 5 cells were formed by centrifugation at 250 g and cultured for 1 or 21 days in chondrogenic induction medium consisting of DMEM-HG supplemented with Glutamax, ITS+1 (B&D Bioscience, Bedford, MA, USA), 40 μg/ml L-proline (Sigma-Aldrich), 1 mM sodium-pyruvate (Gibco, Carlsbad, CA, USA), 10 ng/ml TGFβ1, 10 -4 M ascorbic acid-2-phophate, 5 μg/ml fungizone, 50 μg/ ml gentamicin and 10 -7 M dexamethasone (Sigma-Aldrich). Medium was renewed twice per week.
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