8 iso 15 r pgf2α

Manufactured by Cayman Chemical

Sourced in United States

8-iso-15(R)-PGF2α is a stable isoprostane compound. It is a structural analogue of prostaglandin F2α. This product is intended for laboratory research use only.

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5 protocols using 8 iso 15 r pgf2α

Eicosanoid Standards Protocol

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Standards of F₂-IP (5[R]-iPF_2α-VI, 5[S]-iPF_2α-VI, 5-iPF_2α-VI-d₁₁, 8-iso-15[S]-PGF_2α, 8-iso-15[R]-PGF_2α, 8-iso-15[S]-PGF_2α-d₄, and 15[R]-PGF_2α), 15(S)-PGF_2α (hereafter referred to as PGF_2α), 20:4, and 20:4-d₈ were from Cayman Chemicals (Ann Arbor, MI). Artificial CSF was from Tocris Bioscience (Bristol, UK). Methanol (Fisher Scientific Inc.), hexane, and ethyl acetate (Lab Scan) were of high-performance liquid chromatography grade, and Bond Elut CertifyII SPE columns were from Agilent Technologies (Santa Clara, CA). Other chemicals were from Sigma, unless indicated otherwise.

Lam M.A., Maghzal G.J., Khademi M., Piehl F., Ratzer R., Romme Christensen J., Sellebjerg F.T., Olsson T, & Stocker R. (2016). Absence of systemic oxidative stress and increased CSF prostaglandin F2α in progressive MS. Neurology® Neuroimmunology & Neuroinflammation, 3(4), e256.

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Untargeted Metabolomics Mass Spectrometry

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All solvents for untargeted mass spectrometry were LC-MS grade. Acetonitrile, methanol, and formic acid were purchased from Fisher Scientific (Fairlawn, New Jersey); Water was purchased from Honeywell (Muskegon Michigan); 1-methylhistidine, 3-methylhistidine, α-amino-n-butyric acid, alanine, an serine, arginine, asparagine, aspartic acid, β-aminoisobutryic acid, β-alanine, carnosine, citrulline, creatinine, cystathionine, cysteine, ethanolamine, γ-aminobutyric acid, glutamic acid, glutamine, glycine, histidine, homocystine, hydroxy lysine, hydroxy proline, iso leucine, L-aminoadipic acid, L-cystine, leucine, lysine, proline, methionine, ornithine, phenylalanine, phospho serine, phosphoethanolamine, sarcosine, serine, taurine, threonine, tryptophan, tyrosine, urea, and valine were purchased from Sigma Aldrich (St. Louis, Missouri); 10(S),17(S)-DiHDoHE (Protectin DX), 11β-PGF_2α, 14(S)-HDHA, 15R-PGF_2α, 17(S)-HDHA, 8-iso-15R-PGF_2α, 8-iso-PGF_2α, Lipoxin A4 (LXA₄), LTB₄, LTC₄, LTD₄, LTE₄, PGE₂, PGF_2α, Resolvin D1 (RVD1), and Resolvin D2 (RVD2) were purchased from Cayman Chemical (Ann Arbor, Michigan).
All standards and deuterated internal standards used for LC-MS/MS analysis of arachidonic acid, docosahexaenoic acid derived lipid mediators were purchased from Cayman Chemical (Ann Arbor, Michigan, USA). All HPLC solvents and extraction solvents were HPLC grade or better.

Cruickshank-Quinn C., Armstrong M., Powell R., Gomez J., Elie M, & Reisdorph N. (2017). Determining the presence of asthma-related molecules and salivary contamination in exhaled breath condensate. Respiratory Research, 18, 57.

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Quantification of Isoprostanes in Biological Samples

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Acetonitrile (HPLC grade), methanol (HPLC grade), formic acid (≥ 99.5%), ammonium hydroxide (certified ACS plus), and phosphate-buffered saline (PBS) without calcium and magnesium were purchased from Fisher Scientific (NJ, United States). Water (HPLC grade) was purchased from JT Baker (NJ, United States). Synthetic 8-Iso-prostaglandin F_2α (8-isoprostane; CAS# 27415–26–5), 8-iso-prostaglandin F_2α-d₄ (>99%) (8-isoprostane d₄; CAS# 211105–40–7), ent-8-iso-15(S)-PGF_2α (≥98%) (CAS# 214748–66–0); ent-8-iso-PGF_2α (≥98%) (CAS# 159812–83–6), 8-iso-PGE₁ (≥98%) (CAS# 21003–46–3); PGE₁ (≥98%) (CAS# 745–65–3); 8-iso-15(R)-PGF_2α (≥98%) (CAS# 214748–65–9); PGF_2α (≥98%) (CAS# 551–11–1); and 15(R)-PGF_2α (≥98%) (CAS# 37658–84–7) were obtained from Cayman Chemical Company (MI, United States). Potassium phosphate monobasic crystals (Reagent ACS) was obtained from Acros (NJ, United States). Potassium phosphate buffer with a pH of 6.1 was prepared using a Mettler Toledo S220 pH meter (Greifensee, Switzerland).

Holder C., Adams A., Allison C., Cote O., Lippens R., Blount B.C, & Wang L. (2021). A Novel UHPLC-MS/MS Method for Measuring 8-iso-Prostaglandin F2α in Bronchoalveolar Lavage Fluid. Frontiers in Chemistry, 9, 695940.

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Comprehensive Lipid Standards Analysis

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PGF2α-d9, PGD2-d9, PGE2-d4, PGF1α-d9, PGF2α, 8-iso-PGF2α, ent-PGF2α, ent-8-iso-PGF2α ent-8-iso-15(S)-PGF2α, 15(R)-PGF2α, 8-iso-15(R)-PGF2α, 11β-PGF2α, 8-iso-9β-PGF2α, PGF1α, PGF3α, PGD2, PGE2, PGE1, PGD1, 6-keto-PGF1α, PGA1, and PGA2 standards were obtained from Cayman Chemical (Ann Arbor, MI). All HPLC solvents and reagents were purchased from Fisher Scientific Co. (Norcross, GA) and were of HPLC grade.

Pier B., Edmonds J.W., Wilson L., Arabshahi A., Moore R., Bates G.W., Prasain J.K, & Miller M.A. (2017). Comprehensive profiling of prostaglandins in human ovarian follicular fluid using mass spectrometry. Prostaglandins & other lipid mediators, 134, 7-15.

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Lipidomic Analysis of Oxidative Damage

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M ethanol, NaOH, KOH, ethanol, heptane, acetonitrile and ethyl acetate (analytical grade) were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). The formic acid and HCl were from Panreac (Barcelona, Spain) and Ultrapure H₂O was generated on a milliQ system from Millipore.
Lipid oxidative damage was determined by the analysis of prostaglandines (PG), isoprostanes (IsoP), neuroprostanes (NeuroP), dihomo-IsoP, and dihomo-isofurans (dihomo-IsoF) using ultra high-performance liquid chromatography mass spectrometry (UPLC-MS/MS) (Waters Acquity UPLC-XevoTQ system, Milford, MA, USA). The analysis was carried out on different biological matrices: retina, plasma and urine.
The prostaglandin standard PGE₂, PGF_2α, the IsoP standards 8-iso-PGE₂, 8-iso-15-keto-PGE₂, 8-iso-PGF_2α (also named 15-F_2t-IsoP), 8-iso-15-keto-PGF_2α, 8-iso-15(R)-PGF_2α, 5-iso-PGF_2α–VI, 2,3-dinor-8-iso-PGF_2α, 1a,1b-dihomo-PGF_2α, and deuterated internal standard PGF_2α-d₄ were obtained from Cayman Chemical Company (Ann Arbor, MI, USA) (Figure 1). The standards 10-epi-10-F_4t-NeuroP, 4(RS)-4-F_4t-NeuroP, 14(RS)-14-F_4t-NeuroP, 17-F_2t-dihomo-IsoP, Ent-7(RS)-7-F_2t-dihomo-IsoP, 17-epi-17-F_2t-dihomo-IsoP, 17(RS)-10-epi-SC-Δ¹⁵-11-dihomo-IsoF, 7(RS)-ST-Δ⁸-11-dihomo-IsoF were synthesized at the Institute of Biomolecules Max Mousseron (IBMM) (Montpellier, France) by Professor Durand’s team [15 (link)] (Figure 1).

Torres-Cuevas I., Millán I., Asensi M., Vento M., Oger C., Galano J.M., Durand T, & Ortega Á.L. (2021). Analysis of Lipid Peroxidation by UPLC-MS/MS and Retinoprotective Effects of the Natural Polyphenol Pterostilbene. Antioxidants, 10(2), 168.

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