Aeromonas species consists of oxidase-positive, polar flagellated, glucose-fermenting, facultatively anaerobic, motile bacteria that do not grow in gram-negative rods containing 6.5% NaCl. Conventional methods were used to identify all strains. For further verification, API-20E and ID32 GN Systems (bioMérieux Inc., Hazelwood, MO, USA) or Vitek 2 ID-GNB identification cards (bioMérieux Inc., Durham, NC, USA) were used. Clinical and Laboratory Standards Institute (CLSI) criteria for microorganisms were used to interpret the results of antimicrobial susceptibility tests.
Api 20e and id32 gn systems
Manufactured by bioMérieux
Sourced in United States
The API-20E and ID32 GN Systems are bacterial identification systems used in clinical microbiology laboratories. The API-20E system is designed for the identification of Enterobacteriaceae and other Gram-negative rods, while the ID32 GN system is used for the identification of a wide range of Gram-negative bacteria. Both systems employ biochemical tests to obtain profiles that are then compared to databases for automated identification of bacterial isolates.
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Lab products found in correlation
2 protocols using api 20e and id32 gn systems
1
Identification and Antibiotic Susceptibility of Aeromonas
2
Vibrio and Aeromonas Identification and Antibiotic Resistance
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Identi cation of Vibrio vulni cus, Aeromonas hydrophila and Aeromonas sobria was based on standard phenotypic tests used in clinical microbiology laboratories. All strains were identi ed to the species level by conventional methods and were further veri ed by the API-20E and ID 32 GN Systems (bioMérieux Inc., Hazelwood, MO, USA), or the Vitek 2 ID-GNB identi cation card (bioMérieux Inc., Durham, NC, USA). Antimicrobial susceptibility of Vibrio vulni cus and Aeromonas species was performed by the hospital microbiology laboratory via the standard disk diffusion technique. These antimicrobial susceptibility tests were performed as recommended by the Clinical and Laboratory Standards Institute (CLSI), and the results were interpreted according to the CLSI criteria for these microorganisms. When the antimicrobial susceptibility test of the isolate revealed resistance in more than one antibiotics, the isolate of the patient was recorded as antibiotic resistance case.
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