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Rabbit anti γh2ax antibody

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Rabbit anti-γH2AX antibody is a primary antibody that specifically recognizes the phosphorylated form of the histone H2AX protein, known as γ-H2AX. This antibody is a useful tool for the detection and quantification of DNA double-strand breaks in cells.

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Lab products found in correlation

Axiovert 200m, by Zeiss (4 mentions) Alexa 488 labeled secondary antibody, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Anti-γH2AX antibody (3 citations) γH2AX antibody (8 citations) γH2AX (46 citations) Rabbit anti-TM (2 citations)

2 protocols using rabbit anti γh2ax antibody

1

γH2AX and Telomere Analysis in EBV Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
EBV immortalized cells derived from cryopreserved lymphocytes were spun onto Cytospin microscopic slides at 200 rpm for 3 minutes. Cells were fixed in 2% paraformaldehyde, permeabilized with 0.5% triton X-100, and blocked with 10% FBS for 1-2 hours. Cells were stained with a rabbit anti-γH2AX antibody (1:200, Santa Cruz) 51 (link), followed by Alexa 488-labeled secondary antibody (1:500; Molecular Probes). For combined telomere FISH, slides were fixed in 2% paraformaldehyde and then used to perform telomere-FISH. Z-stack images were captured on a fluorescence microscope (Axiovert 200M; Carl Zeiss). The experiments were repeated three times on each sample.
Shi J., Yang X.R., Ballew B., Rotunno M., Calista D., Fargnoli M.C., Ghiorzo P., Paillerets B.B., Nagore E., Avril M.F., Caporaso N.E., McMaster M.L., Cullen M., Wang Z., Zhang X., Bruno W., Pastorino L., Queirolo P., Banuls-Roca J., Garcia-Casado Z., Vaysse A., Mohamdi H., Riazalhosseini Y., Foglio M., Jouenne F., Hua X., Hyland P.L., Yin J., Vallabhaneni H., Chai W., Minghetti P., Pellegrini C., Ravichandran S., Eggermont A., Lathrop M., Peris K., Scarra G.B., Landi G., Savage S.A., Sampson J.N., He J., Yeager M., Goldin L.R., Demenais F., Chanock S.J., Tucker M.A., Goldstein A.M., Liu Y, & Landi M.T. (2014). Rare missense variants in POT1 predispose to familial cutaneous malignant melanoma. Nature genetics, 46(5), 482-486.
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2

γH2AX and Telomere Analysis in EBV Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
EBV immortalized cells derived from cryopreserved lymphocytes were spun onto Cytospin microscopic slides at 200 rpm for 3 minutes. Cells were fixed in 2% paraformaldehyde, permeabilized with 0.5% triton X-100, and blocked with 10% FBS for 1-2 hours. Cells were stained with a rabbit anti-γH2AX antibody (1:200, Santa Cruz) 51 (link), followed by Alexa 488-labeled secondary antibody (1:500; Molecular Probes). For combined telomere FISH, slides were fixed in 2% paraformaldehyde and then used to perform telomere-FISH. Z-stack images were captured on a fluorescence microscope (Axiovert 200M; Carl Zeiss). The experiments were repeated three times on each sample.
Shi J., Yang X.R., Ballew B., Rotunno M., Calista D., Fargnoli M.C., Ghiorzo P., Paillerets B.B., Nagore E., Avril M.F., Caporaso N.E., McMaster M.L., Cullen M., Wang Z., Zhang X., Bruno W., Pastorino L., Queirolo P., Banuls-Roca J., Garcia-Casado Z., Vaysse A., Mohamdi H., Riazalhosseini Y., Foglio M., Jouenne F., Hua X., Hyland P.L., Yin J., Vallabhaneni H., Chai W., Minghetti P., Pellegrini C., Ravichandran S., Eggermont A., Lathrop M., Peris K., Scarra G.B., Landi G., Savage S.A., Sampson J.N., He J., Yeager M., Goldin L.R., Demenais F., Chanock S.J., Tucker M.A., Goldstein A.M., Liu Y, & Landi M.T. (2014). Rare missense variants in POT1 predispose to familial cutaneous malignant melanoma. Nature genetics, 46(5), 482-486.
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