Palbociclib (S1116), Ribociclib (S7440), Abemaciclib (S7158) and Roscovitine (S1153) were purchased from Selleck Chemicals. Neocarzinostatin (N9162) was obtained from Sigma-Aldrich. CDK2 inhibitor III (238803) and CDK1/2 inhibitor III (217714) were purchased from EMD Millipore Sigma. Trimethoprim (TMP; 16473) was obtained from Cayman Chemical. Anti-phosphor-Rb (Ser807/811) (8516), anti-phosphor-Rb (S608) (2181), anti-p57 (2557) and anti-β-actin (3700) were purchased from Cell Signaling Technology. Anti-phosphor-Rb (S780) (558385), anti-Rb (554136) and anti-p27 (610241) were purchased from BD Bioscience, and anti-phophor-Rb (T373) (ab52975) and anti-cyclin E2 (ab32103) were obtained from Abcam. Anti-Cyclin E (HE12) (sc-247), and anti-GAPDH (FL-335) (sc-25778) were obtained from Santa Cruz.
Anti p27 610241
Manufactured by BD
Sourced in United States
The Anti-p27 (610241) is a laboratory reagent used for the detection and quantification of the p27 protein. It is a monoclonal antibody that specifically binds to the p27 protein, which is a cyclin-dependent kinase inhibitor involved in the regulation of cell cycle progression. This product can be used in various experimental techniques, such as Western blotting, immunohistochemistry, or flow cytometry, to analyze the expression and localization of the p27 protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Abemaciclib, by Selleck Chemicals (1 mentions)
Neocarzinostatin, by Merck Group (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Ribociclib, by Selleck Chemicals (1 mentions)
Roscovitine, by Selleck Chemicals (1 mentions)
Palbociclib, by Selleck Chemicals (1 mentions)
Anti pser473 akt, by Cell Signaling Technology (1 mentions)
Ecl system, by Cytiva (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti phospho s6 ribosomal protein ser240 244 d68f8, by Cell Signaling Technology (1 mentions)
Anti vinculin n 19, by Santa Cruz Biotechnology (1 mentions)
Anti caspase 7, by Cell Signaling Technology (1 mentions)
2 protocols using anti p27 610241
1
CDK Inhibitor Screening Protocol
2
Protein Extraction and Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein extraction and western blotting were performed as described elsewhere.58 (link) Antibodies directed against HMGA1 proteins were already described.59 (link) Commercial antibodies were: anti-pSer473-AKT (#9271), anti-AKT (#9272), anti-Caspase-7 (#9492), anti-phospho S6 ribosomal protein (Ser240/244) (D68F8, #5364), anti-S6 ribosomal protein (54D2, #2317S), anti-LC3A/B (#4108), anti-PARP (46D11, #9532), anti-ATG5 (D5F5U, #12994) and anti-ULK1 (R600, #4773) antibodies from Cell Signaling Technology (MA, USA); anti-β-Actin (I-19, sc-1616), anti-β-Tubulin (D-10, sc-5274), anti-p21 (C-19, sc-397), anti-Cyclin D1 (A12, sc-8396), anti-Cyclin E (E20, sc-481), and anti-Vinculin (N-19, sc-7649) were from Santa Cruz Biotechnology (Dallas, TX, USA); anti-p27 (610241) and anti-p62/SQSTM1 (610833) were from BD Biosciences (Franklin Lakes, NJ USA); anti-phospho ATG13 (Ser318) (PAB19948) was from Abnova (Taipei City,Taiwan); anti-ATG13 (SAB4200100) was from Sigma-Aldrich. ECL System was purchased from Amersham Pharmacia (Buckinghamshire, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!