Horseradish peroxidase conjugated secondary anti igg antibodies

Horseradish peroxidase-conjugated secondary anti-IgG antibodies were from Millipore (Billerica, MA, USA). Anti-GFP rabbit polyclonal serum A6455, Alexa 488-, Alexa 647- and Alexa 594-conjugated secondary antibodies were obtained from Molecular Probes (Eugene, OR, USA). Low-glucose DMEM, fetal bovine serum (FBS), o-nitrophenyl-β-D-galactopyranoside (ONPG), carboxymethylcellulose sodium salt (CMC) medium-viscosity and protease inhibitor cocktail were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Mowiol was from Calbiochem (Merck Chemicals, Germany). Jet-PEI was from Polyplus-transfection (Illkirch, France).
PLP-EGFP was generated by fusing EGFP to the COOH terminus of PLP by gene fusion PCR. The fusion product was cloned into pEGFPN1 vector using the EcoRI–NotI site. Stable cell lines were obtained by the cotransfection of PLP-EGFPNI and pMSCV-hygro (CLONTECH Laboratories, Inc.) followed by the selection of clones by incubation with hygromycin.

Horseradish peroxidase-conjugated secondary anti-IgG antibodies were purchased from Millipore (Billerica, MA, USA). Alexa Fluor 647-conjugated secondary antibody was obtained from Molecular Probes (Eugene, OR, USA). Polyclonal rabbit anti-HSV-1 antibodies were from Dako and Antibodies Online GmbH. Mouse anti-PLP MAB388 and anti-integrin β-1 MAB5199 antibodies were from Millipore. Anti-CNPase C5922, low-glucose DMEM, fetal bovine serum (FBS), human insulin, triiodothyronine (T3), apo-transferrin, sodium selenite, putrescine, dibutyryl cyclic AMP (dbcAMP), and protease inhibitor cocktail were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Anti-CD63 antibody 1B5 (87 (link)) was a kind gift from A. Fraile-Ramos (Universidad Complutense, Madrid, Spain). Anti-CD81 antibody 5A6 (SC-23962) was a kind gift from F. Sánchez-Madrid (Hospital de la Princesa, Madrid, Spain). The rabbit LC3-II antibody was from Novus Biologicals. Anti-flotillin-1 antibody 610821 was from BD Biosciences. Mowiol was from Calbiochem (Merck Chemicals, Germany).

Anti nectin-1 monoclonal antibody CK41 and anti-HVEM polyclonal antibody R140 have been described previously [46] (link), [47] (link). Horseradish peroxidase-conjugated secondary anti-IgG antibodies were purchased from Millipore (Billerica, MA, USA). Anti-green fluorescent protein GFP rabbit polyclonal serum A6455, Alexa 488-, Alexa 647- and Alexa 594-conjugated secondary antibodies were obtained from Molecular Probes (Eugene, OR, USA). DNA size marker was from Invitrogen. Polyclonal rabbit anti-HSV-1 antibody was from DAKO. Monoclonal mouse anti-PLP MAB388 antibody was from Millipore. Anti-nectin-1 mouse monoclonal antibody CK6 was from Santa Cruz Biotechnology. Anti-HVEM mouse monoclonal antibody, low-glucose DMEM, fetal bovine serum (FBS), human insulin, triiodothyronine (T3), apo-transferrin, sodium selenite, putrescine, dibutyryl cyclic AMP (dbcAMP), carboxymethylcellulose sodium salt (CMC) medium-viscosity and protease inhibitor cocktail were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Mowiol was from Calbiochem (Merck Chemicals, Germany). HS4C3 antibody was a kind gift of Dr. R. Longnecker, (Northwestern Medical School, Chicago, USA).