Rabbit polyclonal antibody specific for phosphate p85

Rabbit polyclonal antibody specific for phosphate-p85, a heterodimer of phosphatidylinositol 3 kinase (PI3K), p-Akt, p-IKK were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibodies specific for PI3K, Akt, IKK, NF-κB, β-actin, and mouse polyclonal antibodies specific for OSM were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The recombinant human adiponectin was purchased from PeproTech (Rocky Hill, NJ, USA). PI3K inhibitors (Wortmannin and Ly294002), Akt inhibitor, and NF-κB inhibitors pyrrolidine dithiocarbamate (PDTC) and L-1-tosylamido-2-phenylenylethyl chloromethyl ketone (TPCK) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Small-interfering RNAs (siRNAs) against p85, Akt, and p65 were purchased from Dharmacon Research (Lafayette, CO, USA). OSM ELISA kit was purchased from R&D Systems (Minneapolis, MN, USA). DMEM, fetal bovine serum (FBS), and all the other cell culture reagents were purchased from Gibco life technologies (Grand Island, NY, USA).

Rabbit polyclonal antibody specific for phosphate-p85, a heterodimer of PI3K, p-Akt, and p-PDK1 were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibodies specific for PDK1, PI3K, Akt, c-Jun, β-actin, and mouse polyclonal antibodies specific for IL-18 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Recombinant human HIMF was purchased from PeproTech (Rocky Hill, NJ). Neutralized monoclonal anti-IL-18 and anti-VEGF-A antibodies were both purchased from R&D Systems (Minneapolis, MN, USA). PI3K inhibitors (Ly294002), the Akt inhibitor, and c-Jun inhibitors (Tanshinone IIA) were purchased from Sigma-Aldrich (St. Louis, MO). Those siRNAs against PDK1, p85, Akt, and c-Jun were purchased from Dharmacon Research (Lafayette, CO, USA). Constitutive active plasmids of PI3K and c-Jun were gifts from Lewis Cantley (Addgene plasmid # 1399) and from Axel Behrens (Addgene plasmid # 47443), respectively. Another constitutive active plasmid of Akt was a kind gift from Dr. W.C. Huang (China Medical University, Taiwan). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and all other cell culture reagents were purchased from Gibco Life Technologies (Grand Island, NY, USA).