Three flag leaves were selected from each plot during the grain-filling period on a clear and windless day. The CIRAS-3 photosynthetic measurement system produced by PP Systems (USA) was used to measure the net photosynthetic rate (Pn), transpiration rate (Tr), and leaf water use efficiency (LWUE). The measurement period was from 10:00 to 18:00. Observations were made every 2 h, and average values were determined. A SPAD-502 chlorophyll meter produced by SONY was used to determine the relative content of chlorophyll (SPAD) in the flag leaves.
Ciras 3 photosynthetic measurement system
Manufactured by PP Systems
Sourced in United States
The CIRAS-3 is a photosynthetic measurement system designed to analyze the gas exchange of plants. It provides accurate and reliable data on parameters such as carbon dioxide and water vapor concentrations, as well as other physiological measurements related to plant photosynthesis.
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Lab products found in correlation
2 protocols using ciras 3 photosynthetic measurement system
1
Photosynthesis and Chlorophyll in Flag Leaves
2
Photosynthetic Parameters of Wheat Leaves
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The photosynthetic parameters of single flag leaves at the grain filling stage were measured from 9:00 a.m. to 11:00 a.m. using a Ciras-3 photosynthetic measurement system (PP systems, Amesbury, MA, USA). The leaf chamber temperature was set at 25 °C, and a light intensity of 1200 µmol·m−2·s−1 was used as the supplementary light source to measure Pn, intercellular CO2 concentration (Ci), and gs of the wheat leaves. At least five leaves of each variety were measured.
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