Apc rat igg2a

The following antibodies were used for immunostaining. Anti-Arginase-1 (Santa cruz, sc-20150, 1:200), anti-BMP-2 (Abcam, ab6285, 1:100 or Bioss, bs-1012R, 1:100), anti-β-catenin (Abcam, ab2365, 1:100), anti-caspase-3 (Epitomics, 1476−1, 1:100), anti-F4/80 (Abcam, ab6640, 1:100), anti- HSP47 (Novus, NBP1-97491, 1:50), anti-K15 (Abcam, ab52816 or ThermoFisher, MA5-11344, 1:100), anti-Ki67 (ThermoFisher, RM-9106, 1:100), anti-perilipin-1 (Abcam, ab3526, 1:100), and anti-phospho-Smad1/5 (Cell signaling, 9516, 1:50).
The following antibodies were used for flow cytometry or cell sorting. Anti-CD45 (BD pharmingen, 553081, 1:200), anti-CD86 (eBioscience, 17-0862, 1:400), anti-CD206 (BioLegend, 141706, 1:40), and anti-F4/80 (eBioscience, 17-4801, 1:10). The corresponding isotype controls were APC/Cy7 Mouse IgG2a, κ (BioLegend, 400230), FITC Rat IgG2a, κ (BD pharmingen, 557228), PE Rat IgG2a, κ (BD pharmingen, 557229), and APC Rat IgG2a, κ (BD pharmingen, 551442).

PE mouse IgG1(BD), APC Rat IgG2a (BD), PerCp/Cy5.5 mouse IgG2b (Biolegend), Alexa Fluor 700 Mouse IgG1 (BD), PE-Cy7 Mouse IgG2a (BD), V450 Mouse IgG1 (BD), APC Cy7 Mouse IgG1 (Biolegend), FITC Mouse IgG2a (BD).
Following staining, cells were fixed with 2% paraformaldehyde and run on a BD Biosciences LSR II flow cytometer (San Jose, CA, USA). Data were analyzed using Winlist 6.0 (Topsham, ME, USA). Results are reported as the percent of the phenotype being analyzed in the total peripheral blood mononuclear cell pool.