Rabbit anti rbap48

To examine types of cells containing RbAp48 immunoreactivity, double immunofluorescence staining was performed according to our published protocol (Park and others 2016). In brief, we used rabbit anti-RbAp48 (1:100, abcam, Cambridge, MA, USA)/mouse anti-GFAP (1:400, abcam, Cambridge, MA, USA) for astrocytes or goat anti-Iba1 (1:400, abcam, Cambridge, MA, USA) for microglia. The sections were incubated in the mixture of the antisera, and the incubated sections were reacted in a mixture of both donkey anti-rabbit IgG, Alexa Fluor 546 or 546 (1:500, Invitrogen, Waltham, MA, USA) and goat anti-mouse IgG, Alexa Fluor 488 (1:500, Invitrogen, Waltham, MA, USA) and donkey anti-goat IgG, Alexa Fluor546 (1:500, Invitrogen, Waltham, MA, USA).
The double immunoreaction for RbAp48/astrocytes or RbAp48/microglia was observed under a confocal MS (LSM510 META NLO, Carl Zeiss, Germany) in the Korea Basic Science Institute Chuncheon Center.

Rabbit antibodies against EZH2, histone 3, ubiquitin, H3K9me3 and H3K27me3 were purchased from Upstate Company. Rabbit antibody against EED was a kind gift from Dr. Armin Schumacher. Monoclonal anti-His antibody was purchased from GE Company. Mouse anti-actin antibody was purchased from Chemicon Company. Mouse anti-flag antibody, mouse anti-myc antibody, HRP-conjugated anti-rabbit or anti-mouse IgG antibodies were purchased from Sigma Company. Rabbit anti V5, rabbit anti AEBP2, rabbit anti GluR6, rabbit anti Rbap48 were purchased from Abcam Company. Rabbit anti Nanog antibody purchased from Cell Signaling Inc. Rabbit anti-JAZF1 and SUZ12 antiserum were prepared described [58 (link)] and further affinity-purified using protein A sepharose columns before use. Immunoprecipitation was carried out following manufacture's protocol (Upstate). For western blot analysis, proteins were separated by SDS-PAGE gel electrophoresis, transferred to PVDF membranes, and detected with the indicated antibodies.