Brain tissues were embedded in paraffin and sectioned. Then sections were processed as follows: Xylene for 20 min, two times; 100% ethanol for 5 min, two times; 75% ethanol for 5 min; and tap water rinsing. Sections were subjected to hematoxylin–eosin (HE) and toluidine blue (Nissl) staining. Hematoxylin–Eosin Staining Kit (G1003; Wuhan Servicebio Technology Co., Ltd., Wuhan, China) was used for HE staining, and Toluidine blue staining solution (G1032; Wuhan Servicebio Technology Co., Ltd.) was used for Nissl staining. Sections were finally sealed with neutral gum and examined under an upright optical microscope (NIKON ECLIPSE E100) for image acquisition.
Toluidine blue staining solution
Manufactured by Wuhan Servicebio Technology
Sourced in China
Toluidine blue staining solution is a dye used in various histological and cytological applications. It is a metachromatic stain that can selectively stain different cellular and tissue components. The solution is commonly used to stain nucleic acids, allowing for the visualization and differentiation of cellular structures.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using toluidine blue staining solution
1
Embedding, Staining, and Imaging Brain Tissue
2
Histological Tissue Processing and Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Brain tissues were embedded in paraffin and sectioned. Then sections were processed as follows: Xylene for 20 min, two times; 100% ethanol for 5 min, two times; 75% ethanol for 5 min; and tap water rinsing. Sections were subjected to hematoxylin–eosin (HE) and toluidine blue (Nissl) staining. Hematoxylin–Eosin Staining Kit (G1003; Wuhan Servicebio Technology Co., Ltd., Wuhan, China) was used for HE staining, and Toluidine blue staining solution (G1032; Wuhan Servicebio Technology Co., Ltd.) was used for Nissl staining.
3
Nissl Body Staining in Rat VPL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The rats were anesthetized with isoflurane and perfused with paraformaldehyde, and the brains were removed. The brains were fixed with 4% paraformaldehyde, dehydrated using an ethanol gradient, cleared with xylene, embedded in paraffin, and sectioned. The 5-μm-thick coronal sections were mounted on coverslips, dewaxed using an ethanol gradient, stained with Toluidine Blue staining solution (Servicebio, Wuhan, China), and incubated at 56°C for 20 minutes. Subsequently, they were washed with distilled water, immersed in 70% ethanol for 1 minute, and then immersed in 95% ethanol for 1 minute. Next, the samples were dehydrated quickly with absolute ethanol and rendered transparent with xylene, the cover slips were sealed with neutral gum, and Nissl bodies in the VPL area were imaged using a light microscope (Nikon, Tokyo, Japan).
4
Vaginal Smear Examination for Estrous Cycle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the ovariectomy surgery or induction of estrus, the vaginal smear test was conducted to confirm that they were in or not in estrus. Procedures were as follows: (1) a moist cotton swab with a diameter of 0.5 to 1.0 cm was inserted into a female rat’s vagina and gently rotated several times; (2) the cotton swab was removed from vagina and applied lightly on a glass slide and waiting to air dry at room temperature; (3) the glass slide was put in toluidine blue staining solution (ServiceBio) for 3-10 minutes after it was completely dry; (4) the stained slides were taken out and excess dye were washed off with clean water until they were dry; and (5) the smears were viewed under the microscope and judgments were made according to the typical characteristic of each estrous cycle18 (link) (Supplementary Figure 1C-E ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!