Log In Sign up
The largest database of trusted experimental protocols

The CAND1 is a laboratory instrument designed for DNA and RNA quantification and analysis. It utilizes fluorescence-based technology to accurately measure the concentration and purity of nucleic acid samples. The CAND1 provides reliable and reproducible results, enabling researchers to make informed decisions about their experimental workflows.

Automatically generated - may contain errors

Lab products found in correlation

Usp7 sirna, by Integrated DNA Technologies (2 mentions) On targetplus sirna, by Horizon Discovery (2 mentions) On targetplus smartpool sirna, by Horizon Discovery (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)

2 protocols using cand1

1

Knockdown of AKTIP, SGK3 and STAT3

Check if the same lab product or an alternative is used in the 5 most similar protocols
ON-TARGETplus siRNA targeting human AKTIP, SGK3 and STAT3 as well as SMARTpool ON-TARGETplus siRNA targeting 10 AKTIP-bound proteins were purchased from Dharmacon (Lafayette, CO). ESR1, CAND1, FASN and USP7 siRNA were obtained from Integrated DNA Technologies (Coraville, IA). The siRNA sequences are listed in Table S3. Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA) was used to transfect siRNA at 10 nM. shRNA against AKTIP in pLKO.1-Puro lentiviral vector was developed by The RNAi Consortium (TRC).87 (link) pcDNA3-myc3-CUL2 and pcDNA3-myc3-NEDD8 were gifts from Yue Xiong (Addgene plasmid # 19892 and # 19943).84 (link),85 (link) Transfection of plasmids was performed using Lipofectamine 3000 (Invitrogen). MCF7 cells stably expressing AKTIP shRNA or vector control were established by lentiviral transduction followed by puromycin selection.
Ng A.S., Zhang S., Mak V.C., Zhou Y., Yuen Y., Sharma R., Lu Y., Zhuang G., Zhao W., Pang H.H, & Cheung L.W. (2022). AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance. Cell reports, 41(11), 111821.
+ Open protocol
+ Expand
2

Knockdown of AKTIP, SGK3 and STAT3

Check if the same lab product or an alternative is used in the 5 most similar protocols
ON-TARGETplus siRNA targeting human AKTIP, SGK3 and STAT3 as well as SMARTpool ON-TARGETplus siRNA targeting 10 AKTIP-bound proteins were purchased from Dharmacon (Lafayette, CO). ESR1, CAND1, FASN and USP7 siRNA were obtained from Integrated DNA Technologies (Coraville, IA). The siRNA sequences are listed in Table S3. Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA) was used to transfect siRNA at 10 nM. shRNA against AKTIP in pLKO.1-Puro lentiviral vector was developed by The RNAi Consortium (TRC).87 (link) pcDNA3-myc3-CUL2 and pcDNA3-myc3-NEDD8 were gifts from Yue Xiong (Addgene plasmid # 19892 and # 19943).84 (link),85 (link) Transfection of plasmids was performed using Lipofectamine 3000 (Invitrogen). MCF7 cells stably expressing AKTIP shRNA or vector control were established by lentiviral transduction followed by puromycin selection.
Ng A.S., Zhang S., Mak V.C., Zhou Y., Yuen Y., Sharma R., Lu Y., Zhuang G., Zhao W., Pang H.H, & Cheung L.W. (2022). AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance. Cell reports, 41(11), 111821.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!