7693 autosampler

Manufactured by Agilent Technologies

Sourced in United States, Australia

The 7693 autosampler is a laboratory equipment manufactured by Agilent Technologies. Its core function is to automatically inject samples into an analytical instrument, such as a gas chromatograph, for analysis.

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Lab products found in correlation

52 protocols using 7693 autosampler

Quantitative GC-MS/MS Analysis of PCBs

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GC-MS/MS (Agilent 7890 A GC system, Agilent 7000 Triple Quad, Agilent 7693 autosampler) in multiple reaction monitoring mode (MRM) was used for identification and quantification of 209 PCBs as 174 chromatographic peaks. The GC was equipped with a Supelco SPB-Octyl capillary column (50% n-octyl, 50% methyl siloxane, 30 m × 0.25 mm ID, 0.25 μm film thicknesses) with helium as the carrier gas flowing at 0.75 mL/min and nitrogen/argon as the collision gas. The GC operated in solvent vent injection mode at the following injection conditions: initial temperature 45 °C, initial time 0.06 min, ramp 600 °C/min to inlet temperature 325 °C at 4.4 psi. The GC oven temperature program was 45 °C for 2 min, 45–75 °C at 100 °C/min and hold for 5 min, 75–150 °C at 15 °C/min and hold for 1 min, 150 to 280 at 2.5 °C/min and final hold 5 min (total run time 70.86 min). The triple quadrupole MS electron ionization source was set to 260 °C. Additional details can be found in the supporting information (Tables S3–S13).

Bako C.M., Mattes T.E., Marek R.F., Hornbuckle K.C, & Schnoor J.L. (2020). Biodegradation of PCB congeners by Paraburkholderia xenovorans LB400 in presence and absence of sediment during lab bioreactor experiments. Environmental pollution (Barking, Essex : 1987), 271, 116364.

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Quantifying 1-Octanol and Octyl Acetate

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Hexadecane solvent overlay was used to capture 1-octanol and octyl acetate from the liquid culture. Alternatively, 0.1 volume of hexadecane was used to extract samples of the aqueous phase from mock or production cultures. One hundred microliters of hexadecane overlay or extract was transferred into an insert in a 2-mL screw top GC vial (Agilent). Samples were analyzed using an Agilent 7890B gas chromatograph with an HP-5 ms column, a 7693 autosampler, and a 5977B MSD system. Then 1 µL of sample was injected using a pulsed split ratio of 10:1 and split flow at 10 min/mL. The GC was programmed with an initial temperature of 70 °C for 30 s, followed by a first ramp at 30 °C/min to 250 °C before ramping up to 300 °C with a final hold for 2 min at 40 °C/min. Target products were identified by comparing mass spectra and retention times with external standards. Serial dilutions of 1-octanol (≥99%; Sigma-Aldrich) and octyl acetate (≥99%; ACROS Organics) standards were used to quantify the concentrations of 1-octanol and octyl acetate in the sample.

Sattayawat P., Yunus I.S, & Jones P.R. (2020). Bioderivatization as a concept for renewable production of chemicals that are toxic or poorly soluble in the liquid phase. Proceedings of the National Academy of Sciences of the United States of America, 117(3), 1404-1413.

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GC-MS Analysis of Chemical Compounds

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The GC–MS analysis was performed using a 7890B gas chromatograph equipped with a 7693 autosampler and a 5977B mass-selective detector (Agilent, Santa Clara, CA, USA). The capillary column used was HP-5MS, 30 m × 0.25 mm × 0.25 mm, from Agilent (Santa Clara, CA, USA). Helium was used as carrier gas at 1.5 mL/min. The split–splitless injector was operated in pulsed pressure splitless mode as follows: initial pressure 0.2 MPa (30 p.s.i.) for 1.3 min, decreased to constant flow. The purge valve was opened after 1.5 min. The injection volume was 5 µL. The temperatures of the GC system were the following: injector temperature 290 °C; transfer line temperature 280 °C; oven temperature program: 50 °C (1.5 min)–30 °C/min–180 °C–20 °C/min–280 °C (20 min). MS detector (quadrupole) was operated in the EI mode at 70 eV with a mass scan range of 50–450 m/z and the sampling rate of 3.6 scans/s.

, & Dąbrowski Ł. (2020). Evaluation of a Simplified Method for GC/MS Qualitative Analysis of Polycyclic Aromatic Hydrocarbons, Polychlorinated Biphenyls, and Organic Pesticides Using PARADISe Computer Program. Molecules, 25(16), 3727.

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Oasis 96-well Plate HLB Extractions

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The extraction plate manifold used for Oasis 96-well plate HLB extractions was purchased from Waters Corporation (Milford, MA). The Savant DNA110 SpeedVac^® used for concentrating samples was purchased from Thermo Fisher Scientific (Waltham, MA). The Agilent 7890 A GC, LTM Series II Fast GC Module, 7000 A GC-MS/MS Triple Quadrupole, 7693 Autosampler and 7697 A Headspace Sampler were purchased from Agilent Technologies. For GC-MS/MS data acquisition and processing, Agilent MassHunter workstation software included Acquisition, Qualitative and Quantitative Analysis.

Farthing D.E., Buxbaum N.P., Lucas P.J., Maglakelidze N., Oliver B., Wang J., Hu K., Castro E., Bare C.V, & Gress R.E. (2017). Comparing DNA enrichment of proliferating cells following administration of different stable isotopes of heavy water. Scientific Reports, 7, 4043.

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GC-MS Analysis of ZTOI Compound Identification

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GC–MS analysis was performed using an Agilent 7890A GC System twinned with an Agilent 5975C inert MSD with Triple‐Axis Detector. The GC column configuration used comprised an Agilent HP‐5MS Ultra Inert (30 m × 250 μm × 0.25 μm) column for chromatographic separation. The injection port was set to 270°C without split mode. Helium was used as carrier gas with constant flow rate of 1.0 ml/min. The oven was initially held at 50°C for 1 min then ramped to 170°C at 10°C/min and to 230°C at 5°C/min. The auxiliary temperature was programmed at 250°C. Liquid injections (1 μl) were achieved using an Agilent 7693 Autosampler. The mass spectrometer high‐efficiency EI source was set to 230°C, and quadrupole was set at 150°C. Mass spectra were recorded in full‐scan mode ranging from 45–600 amu in positive ion mode after a solvent delay of 3 min. The components of ZTOI were identified using library from the National Institute of Standards and Technology 17.

Yang Z., Wang Z., Li J., Long J., Peng C, & Yan D. (2022). Network pharmacology‐based dissection of the underlying mechanisms of dyspnoea induced by zedoary turmeric oil. Basic & Clinical Pharmacology & Toxicology, 130(5), 606-617.

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Pesticides Residue Analysis via GC-MS

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The Agilent 7890A Gas Chromatography-Mass Spectrometer (GC-MS), which is equipped with 7693 auto-sampler coupled with a 7000B triple quadrupole M.S. system was used in the detection and in quality assurance of pesticides residues. A fused silica DB35 capillary column, 30mm long with 0.25 mm internal diameter and 0.25 μm film operating at a range of 50 °C to 360 °C was used with the internal temperature set at 50°C for 1 minute, constantly raised to 150 °C at a rate of 50°C per minute, followed by 280 °C at a heating rate of 5 °C per minute and held for four minutes. The injector temperature was 250°C, and a carrier gas was helium (99.9%) at a flow rate of 1.2 ml min^-1 splitless injection. The injection volume was 1μl at a pressure of 43.193 Psi. The MS ion source temperature was 250°C operated in full scan mode at a scan range of 50–550°C atomic mass unit.

Kapeleka J.A., Sauli E., Sadik O, & Ndakidemi P.A. (2020). Co-exposure risks of pesticides residues and bacterial contamination in fresh fruits and vegetables under smallholder horticultural production systems in Tanzania. PLoS ONE, 15(7), e0235345.

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GC-MS Analysis of Root Metabolites

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GC-MS analyses were performed using Agilent 5975C mass selective detector coupled to a 7890A gas chromatograph (Agilent Technologies, Santa Clara, CA, United States) with 7693 autosampler and a DB-5MS DG capillary column (30 m plus 10 m Duraguard^®) with a diameter of 0.25 mm, film thickness of 0.25 μm (Agilent J &W, Santa Clara, CA, United States) as described by Mamer et al. (2013) (link). The GC-MS was run in electron ionization mode (70 eV) and Selected Ion Monitoring (SIM) mode. Data acquisition was done in Scan and SIM modes using MassHunter (Agilent) software. The spectra obtained were compared against the NIST (National Institute of Standards) database. The root samples had large amounts of malic and citric acids, and were diluted 1:40 before being run again in Scan mode. While root exudates did not require dilution. Data were represented as normalized area which is the area of peak divided by amount of sample in mg (roots) or μL (root exudates).

Sharma M., Saleh D., Charron J.B, & Jabaji S. (2020). A Crosstalk Between Brachypodium Root Exudates, Organic Acids, and Bacillus velezensis B26, a Growth Promoting Bacterium. Frontiers in Microbiology, 11, 575578.

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Stability Analysis of Oxidation Reaction

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Stability samples stored
at 37 °C after 6 months, and samples of the oxidation reaction
using mCPBA were analyzed using GC–MS/SPME.
Analyses were conducted using the Agilent 7980A GC coupled with a
5975C MS detector and a 7693 autosampler (Agilent, Santa Clara, CA).
The column used was a DB-5MS column (30 m × 250 μm ×
0.1 μm, Agilent, Santa Clara, CA) with helium as the carrier
gas. The SPME fiber used was a polydimethylsiloxane fiber (100 μm,
1 cm in length, 24 Ga purchased from Agilent Santa Clara, CA) with
the 24 Ga fiber assembly (Supelco, Bellefonte, PA). The fiber was
exposed to the sample for 30 s at room temperature (22 °C) and
was then desorbed in the injector at a temperature of 180 °C.
The split ratio was 600:1. The GC oven temperature was programmed
to hold at 50 °C for 1 min, then was elevated at a rate of 60
°C/min up to 280 °C and held for 3 min. The MS was operated
in the scan mode. The data obtained from GC–MS/SPME analysis
were analyzed using the ChemStation software (Agilent, Santa Clara,
CA).

Warnakula I.K., Ebrahimpour A., Li S.Y., Gaspe Ralalage R.D., Hewa-Rahinduwage C.C., Kiss M., Rios C.T., Kelley K.D., Whiteman A.C., Thompson D.E., Rockwood G.A, & Petrikovics I. (2020). Evaluation of the Long-Term Storage Stability of the Cyanide Antidote: Dimethyl Trisulfide and Degradation Product Identification. ACS Omega, 5(42), 27171-27179.

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GC-MS Analysis of Zearalenone Analogues

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The GC-MS analysis was carried on an Agilent Technologies 7890A Series gas chromatograph equipped with a 7000A Triple Quad and a 7693 Autosampler (Agilent Technologies, Santa Clara, CA, USA). The DB-5 MS capillary column (0.25 mm × 30 m, 0.25 µm) employed to separate the analytes was purchased from Waters (Milford, MA, USA). The injected sample volume was 2 µL for a single analysis. The injector and detector temperatures were 250 °C and 230 °C, respectively. The oven temperature program was as follows: the initial temperature was 120 °C, and the temperature was increased to 280 °C at a rate of 15 °C/min and held for 5.2 min.
The electron impact ion source (EI) was used, the electron impact energy was 70 ev, the ion source temperature was 230 °C, the interface temperature was 280 °C, the solvent delay was 5 min, and single-ion monitoring mode (SIM) was used with an interval of 0.3 s. The qualitative and quantitative ions of the ZENs are shown in Table 6.

Luo S., Liu Y., Guo Q., Wang X., Tian Y., Yang W., Li J, & Chen Y. (2022). Determination of Zearalenone and Its Derivatives in Feed by Gas Chromatography–Mass Spectrometry with Immunoaffinity Column Cleanup and Isotope Dilution. Toxins, 14(11), 764.

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Pyrethroid Analysis by GC-μECD

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Pyrethroid analysis was performed using an Agilent 7890A Gas Chromatograph with an Agilent 7693 autosampler and a ⁶³Ni-micro electron capture detector (μECD). The chromatograph was equipped with a Supelco SLB-5ms column (30 m x 0.25 mm x 0.25 μm film thickness) (Supelco, St. Louis, Missouri). Temperatures of the inlet and detector were set at 240 °C and 250 °C, respectively. The temperature program was set to the following parameters: hold at 50 °C for 1 min, 15 °C /min to 220 °C, 1 °C /min to 240 °C, hold for 12 min, 15 °C /min to 280 °C, and hold for 10 min. Helium flow was 1.0 ml/min, and makeup flow was 60 ml/min. The detector response was linear ( R² ≥ .995) for all analytes (cis-permethrin, trans-permethrin, α-cypermethrin, and deltamethrin) over the entire concentration range encountered in this study (0.1–4000 ng).

Elser B.A., Simonsen D., Lehmler H.J, & Stevens H.E. (2022). Maternal and fetal tissue distribution of α-cypermethrin and permethrin in pregnant CD-1 mice. Environmental advances, 8, 100239.

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