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3 protocols using 11 amino 1 undecanethiol hydrochloride

1

Synthesis of Thiol-based Molecules

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8-Amino-1-octanethiol hydrochloride (AOT), 6-mercapto-1-hexanol (MOH) and 1-undecanethiol (1-UDT) were purchased from Sigma-Aldrich, 11-amino-1-undecanethiol hydrochloride (AUT) was acquired from Dojindo. All the other chemicals were purchased from Sigma-Aldrich and were of the highest purity available. Solutions were prepared using deionized water form a Milli-Q® Water Purification System (Merck Millipore).
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2

Purification and Characterization of Redox Proteins

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6-amino-1-hexanethiol hydrochloride and 11-amino-1-undecanethiol hydrochloride were purchased from Dojindo; all other chemicals were purchased from Sigma-Aldrich. The reagents were analytical grade and used without further purification. Solutions were prepared with deionized water (18MΩ.cm) from a Millipore MilliQ water purification system. Mhcd1 (100 μM in 50 mM Tris-HCl buffer, pH 7.6, unless stated otherwise) and cyt c552 (150 μM in 50 mM Tris-HCl buffer, pH 8) were purified from M. hydrocarbonoclasticus cells as previously described [26 (link),27 (link)].
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3

Preparation of Thiol-Modified Lipid Membranes

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6-mercapto-1-hexanol, 6-mercaptohexanoic acid, 11-mercapto-1-undecanol, 11-mercaptoundecanoic acid, 11-mercaptoundecylphosphoric acid, (11-mercaptoundecyl)-N,N,N-trimethylammonium bromide and horse heart cytochrome c (Cyt-c) were purchased from Sigma-Aldrich. 11-amino-1-undecanethiol hydrochloride was purchased from Dojindo. 1,2-dioleoyl-sn-glycero-3-phosphocholine (PC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (PE) were purchased from Avanti Polar Lipids. Hydrogen peroxide was purchased from Mallinckrodt Baker, Inc. Amplex Red Ultra was purchased from Invitrogen. The M80A mutant of Cyt-c was prepared according to published procedures.39 (link)
All chemicals were of the highest available purity and were used without further purification. The water used in all experiments was purified by a Millipore system and its resistance was 18.2 MΩ.
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