Balb c h 2d

Manufactured by Charles River Laboratories

Sourced in Germany, China, United States, Japan, France

BALB/c (H-2d) is a widely used inbred mouse strain that expresses the H-2d major histocompatibility complex haplotype. This mouse strain is commonly used in various areas of biomedical research.

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BALB/c (373 citations) BALB/c (H-2d) mice (20 citations) Female BALB/c (H-2d), (3 citations) BALB/c females (H-2d; (2 citations)

42 protocols using balb c h 2d

PvRMC-MSP1 and PvMSP1 Immunization in Mice

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All animal experiments and procedures were performed in accordance with guidelines and approved by the Emory University’s Institutional Animal Care and Use Committee. Female BALB/c (H-2^d), and C57BL/6 (H-2^b) mice, 6 to 8 weeks of age, were purchased from Charles River (Wilmington, MA). The mice were immunized subcutaneously on days 0, 20 and 40, in the base of the tail and the interscapular area, using 20 μg of PvRMC-MSP1 or PvMSP1₁₉ proteins emulsified in Montanide ISA 51 (Seppic, Fairfield, NJ). Mice in the control groups received PBS alone emulsified in the same adjuvant.

Fonseca J.A., Cabrera-Mora M., Singh B., Oliveira-Ferreira J., da Costa Lima-Junior J., Calvo-Calle J.M., Lozano J.M, & Moreno A. (2016). A chimeric protein-based malaria vaccine candidate induces robust T cell responses against Plasmodium vivax MSP119. Scientific Reports, 6, 34527.

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Comparative Study of C57BL/6 and Balb/c Mice

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Eight to ten week-old female C57BL/6 (B6, H2b) and Balb/c (H2d) mice were all obtained from Charles River Laboratories (Calco, Milan, Italy). For details, see the Supplemental Methods.

Dander E., De Lorenzo P., Bottazzi B., Quarello P., Vinci P., Balduzzi A., Masciocchi F., Bonanomi S., Cappuzzello C., Prunotto G., Pavan F., Pasqualini F., Sironi M., Cuccovillo I., Leone R., Salvatori G., Parma M., Terruzzi E., Pagni F., Locatelli F., Mantovani A., Fagioli F., Biondi A., Garlanda C., Valsecchi M.G., Rovelli A, & D'Amico G. (2016). Pentraxin 3 plasma levels at graft-versus-host disease onset predict disease severity and response to therapy in children given haematopoietic stem cell transplantation. Oncotarget, 7(50), 82123-82138.

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Mouse Anesthesia for Ophthalmic Research

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Eight- to 10-week-old male BALB/c (H-2^d) and C57BL/6 (H-2^b) mice were obtained from Charles River Laboratories, Wilmington, MA. Animals were kept in a pathogen-free environment at the Schepens Eye Research Institute Animal Care Facility. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Schepens Eye Research Institute, and were conducted in accordance with the Association for Research in Vision and Ophthalmology (ARVO) statement for the Use of Animals in Ophthalmic and Visual Research. Mice were anesthetized for surgical procedures using intraperitoneal (i.p.) injections of 120 mg/kg Ketamine and 20 mg/kg Xylazine.

Tahvildari M., Emami-Naeini P., Omoto M., Mashaghi A., Chauhan S.K, & Dana R. (2017). Treatment of donor corneal tissue with immunomodulatory cytokines: a novel strategy to promote graft survival in high-risk corneal transplantation. Scientific Reports, 7, 971.

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B Cell Depletion in Murine Models

Cited 1 time

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C57BL/6 (H-2b) and BALB/c (H-2d) mice were obtained from Charles River Laboratories (Sulzfeld, Germany). C57BL/6 Rag1^–/– mice were originally obtained from Irmgard Förster (University Munich, Germany). BALB/c mb1-MerCreMer mice (Cd79a^{tm2[cre/Esr1*]Reth}, MGI:4414764) were a gift from Siegfried Weiss (Helmholtz Centre for Infection Research, Braunschweig, Germany) and were used as homozygous mutant mice in which B cells are completely absent due to the absence of CD79a/Igα (25 (link)). C57BL/6 mb1-cre mice (Cd79a^tm1[cre]Reth, MGI:3687451) were also used as homozygous mutant mice as BM donor mice in 1 experiment. Mice were used at 8–14 weeks of age and were maintained in a specific pathogen–free environment in the animal facility at the Franz-Penzoldt-Zentrum (University Erlangen).

Seefried M., Hundhausen N., Kroeger I., Büttner-Herold M., Hoffmann P., Edinger M., Ullrich E., Berberich-Siebelt F., Britt W.J., Mach M, & Winkler T.H. (2023). Murine cytomegalovirus reactivation concomitant with acute graft-versus-host disease is controlled by antibodies. JCI Insight, 8(5), e149648.

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Heterotopic Heart Transplantation in Mice

Cited 1 time

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Eight to ten-week-old male C57BL/6J (B6; H-2b) and BALB/c (H-2d) mice were purchased from Charles River (Beijing, China). All mouse experiments were performed in a specific pathogen-free facility according to the guidelines of the animal care and use committee of Huazhong University of Science and Technology and complied with the National Institutes of Health (NIH) Guidelines for the care and use of laboratory animals. We established a murine heterotopic heart transplantation model as previously described 10 (link), animals were anesthetized using 2% isoflurane and administered via nose cone mask. All animals were euthanized using CO₂ asphyxiation, followed by cervical dislocation to obtain tissue samples. In the allograft group, BALB/c hearts were transplanted into fully MHC-mismatched B6 recipients. In the control group, B6 hearts were transplanted into MHC-matched B6 recipients.

Chen Z., Xu H., Li Y., Zhang X., Cui J., Zou Y., Yu J., Wu J, & Xia J. (2022). Single-Cell RNA sequencing reveals immune cell dynamics and local intercellular communication in acute murine cardiac allograft rejection. Theranostics, 12(14), 6242-6257.

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C57BL/6 and BALB/c Mice for Kidney Allografts

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Eight-to-ten-week-old female C57BL/6 (BL6, H-2^b) and BALB/c (H-2^d) mice were obtained from Charles River (Calco, Italy). BL6 mice were used as either BM donors or as recipients of kidney allografts from BALB/c donors. All animal experiments were approved by the Institutional Animal Care and Use Committee and were conducted in conformity with the Institutional Guidelines in compliance with national (D.L. n.26 march 4, 2014, G.U., number 61, March 14, 2014) and International Law and Policies (EEC Council Directive 2010/63/EU, 9/22/2010; Guide for the Care and Use of Laboratory Animals, National Academy Press, 1996). All efforts were made to minimize the number of animals used and their suffering.

Casiraghi F., Todeschini M., Azzollini N., Cravedi P., Cassis P., Solini S., Fiori S., Rota C., Karachi A., Carrara C., Noris M., Perico N, & Remuzzi G. (2019). EFFECT OF TIMING AND COMPLEMENT RECEPTOR ANTAGONISM ON INTRAGRAFT RECRUITMENT AND PRO-TOLEROGENIC EFFECTS OF MESENCHYMAL STROMAL CELLS IN MURINE KIDNEY TRANSPLANTATION. Transplantation, 103(6), 1121-1130.

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Mouse Tumor Injection and Treatment

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Five-to-seven week-old female C57BL/6 (H-2^b) and BALB/c (H-2^d) mice were purchased from Charles River Japan (Kanagawa, Japan). Female (C57BL/6 × BALB/c) F1 mice (H-2^b/d) were purchased from Japan SLC, Inc. (Hamamatsu, Japan), and were housed under sterilized conditions. Animal studies were carried out according to the Guideline for Animal Experiments of the National Cancer Center Research Institute and approved by the Institutional Committee for Ethics in Animal Experimentation. Pan02 (5 × 10⁶) and CT26 cells (1 × 10⁶) were injected subcutaneously into the legs of C57BL/6 and BALB/c mice, respectively. When a subcutaneous tumor was established (˜1.0 cm in a diameter), it was directly injected once with Ad-mIFN or control vector (Ad-AP). The tumor volume was calculated using the formula: tumor volume = 1/2 × ([the shortest diameter]² × [the longest diameter]). Data are presented as mean ± standard deviation (SD).

Aida K., Miyakawa R., Suzuki K., Narumi K., Udagawa T., Yamamoto Y., Chikaraishi T., Yoshida T, & Aoki K. (2014). Suppression of Tregs by anti-glucocorticoid induced TNF receptor antibody enhances the antitumor immunity of interferon-α gene therapy for pancreatic cancer. Cancer Science, 105(2), 159-167.

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Transgenic mouse models for HY-specific T cells

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Normal B6 (H-2^b) and BALB/c (H-2^d) mice were from Charles River. Female Rag2^-/- Marilyn mice, transgenic for a TCR (Tcrav1.1, Tcrbv6) specific for the male H-Y peptide NAGFNSNRANSSRSS presented by I-A^b, were obtained from O. Lantz (Curie Institute, Paris, France). Rag2^-/- Il2rg^-/- B6 mice were obtained from J. P. Di Santo (Pasteur Institute, Paris, France). Pdcd1^-/- and GFP⁺ Marilyn mice were obtained by crossing Marilyn mice with Pdcd1^-/- B6 (BIO Resource Center, RIKEN) and GFP⁺ B6 (The Jackson Laboratories), respectively. Cd3e^-/- B6 mice were from The Jackson Laboratories. All mice used in this study were issued from breeding pairs housed in specific pathogen-free conditions (FELASA) at the Institute for Medical Immunology. Experimental animal protocols were performed in accordance with the Animal Care and Use Committee guidelines of the Université Libre de Bruxelles.

Bettonville M., d'Aria S., Weatherly K., Porporato P.E., Zhang J., Bousbata S., Sonveaux P, & Braun M.Y. (2018). Long-term antigen exposure irreversibly modifies metabolic requirements for T cell function. eLife, 7, e30938.

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Lineage Tracing and Depletion of Lymphatic Vessels

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Itgax-Cre-EGFP, LYVE-1-EGFP-Cre and ROSA26.iDTR were purchased from The Jackson Laboratory. C57BL/6 (H-2b) and Balb/c (H-2d) mice (6-8 weeks) were purchased from Charles River (Beijing, China). Selective expressions of EGFP in the nuclei of LECs were obtained using the LYVE-1-EGFP-Cre mice in a C57BL/6 background. LYVE-1-Cre/iDTR mice were generated with intercrossing LYVE-1-Cre mice with ROSA26.iDTR and ablation of LYVE-1⁺ LVs were generated by intravenous diphtheria toxin (Sigma-Aldrich, D0564) at 30ng/mouse/day with 5 consecutive days before renal transplantation. Animal breeding and all experimental procedures were approved by the animal protection and Research Advisory Committee of the First Affiliated Hospital, School of Medicine, Zhejiang University.

Lin J., Chen Y., Zhu H., Cheng K., Wang H., Yu X., Tang M, & Chen J. (2021). Lymphatic Reconstruction in Kidney Allograft Aggravates Chronic Rejection by Promoting Alloantigen Presentation. Frontiers in Immunology, 12, 796260.

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BALB/c and C57BL/6 Mouse Experiments

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Six-week-old BALB/c (H-2d) and C57BL/6 (H-2b) male mice were purchased from Charles River Laboratories (Wilmington, MA, USA). Mice were housed in the Schepens Eye Research Institute animal vivarium and treated according to the guidelines set forth by the Association for Research in Vision and Ophthalmology (ARVO). All animal experiments were reviewed and approved by the Institutional Animal Care and Use Committee.

Inomata T., Mashaghi A., Di Zazzo A., Lee S.M., Chiang H, & Dana R. (2017). Kinetics of Angiogenic Responses in Corneal Transplantation. Cornea, 36(4), 491-496.

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