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Image pro plus software

Manufactured by Olympus
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Image-Pro Plus is an advanced image analysis software developed by Olympus. It provides a comprehensive set of tools for capturing, processing, and analyzing digital images from a variety of sources, including microscopes, cameras, and other imaging devices. The software's core function is to enable users to perform quantitative measurements, data extraction, and image enhancement on their digital images.

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Lab products found in correlation

Chemidoc xrs imager, by Bio-Rad (5 mentions) Bx51 microscope, by Olympus (3 mentions) Matrigel, by BD (2 mentions) Fluorescence microscope, by Olympus (2 mentions) Hrp conjugated goat anti rabbit igg, by Maixin Group (2 mentions) Fluorescence dye conjugated secondary antibodies, by Jackson ImmunoResearch (2 mentions) Appropriate fluorescence dye conjugated secondary antibodies, by Jackson ImmunoResearch (2 mentions) Iba 1, by Abcam (2 mentions) Ix70 microscope, by Olympus (2 mentions) Growth factor reduced matrigel, by BD (2 mentions) Mouse anti mitf, by Thermo Fisher Scientific (2 mentions) Bca protein assay kit, by Beyotime (2 mentions) Super elc detection reagent, by Beyotime (2 mentions) Rabbit anti tyrp1, by Abcam (2 mentions) Mouse anti neun, by Merck Group (2 mentions) Andor spinning disc confocal microscope, by Oxford Instruments (1 mentions) Bx61 microscope, by Olympus (1 mentions) Bx ucb, by Olympus (1 mentions) Fluorescent microscope, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Meilun (1 mentions)

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Image-Pro Plus (52 citations) Image-Pro (2 citations)

86 protocols using image pro plus software

1

Immunohistochemistry and Immunocytochemistry Protocols

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IHC analysis and H-scoring of the FFPE tissue sections was performed as described earlier [57 (link)]. Images were captured using an Olympus BX61 microscope using image pro plus software (Olympus, Center Valley, PA, USA) at 10 × and 20 × optical magnifications. The human samples contained a collection of grade II, III and IV tumor sections (n = 38) with adjacent normal portions wherever possible.
For ICC, cells were seeded on coverslips and processed as described earlier [52 (link)]. Epifluorescence images were captured using an Olympus BX61 microscope and image pro plus software (Olympus) and confocal images with an Andor spinning disc confocal microscope (Andor Technology, Belfast, UK) and Andor iXon 8797 EMCCD camera using andor iq2 software, both at 60 × optical magnification.
Bhattacharya S, & Ghosh M.K. (2014). HAUSP, a novel deubiquitinase for Rb – MDM2 the critical regulator. The Febs Journal, 281(13), 3061-3078.
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2

Intestinal Morphology Analysis

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The intestinal morphology was analyzed as previously described by Dong et al. [38 (link)] with minor modifications. Briefly, 2 cm-long segments of duodenum (about 4 cm from pyloric sphincter) and ileum (about 15 cm beyond ileocecal junction) were harvested, fixed in paraformaldehyde, dehydrated using a graded series of ethanol and embedded in paraffin. Cross sections (5 microns in size) were cut, dehydrated, stained with hematoxylin and eosin (HE). For each section, villus length, crypt depth and villus width were determined with an optical binocular microscope (Olympus BX5, Olympus Optical Co. Ltd, Japan) and Image-Pro Plus software. The villi/crypt ratio and villus area were calculated.
Wang C., Zhang L., Su W., Ying Z., He J., Zhang L., Zhong X, & Wang T. (2017). Zinc oxide nanoparticles as a substitute for zinc oxide or colistin sulfate: Effects on growth, serum enzymes, zinc deposition, intestinal morphology and epithelial barrier in weaned piglets. PLoS ONE, 12(7), e0181136.
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Histopathological Analysis of Renal and Liver Tissues

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Finally, the rats were euthanized under sodium pentobarbital anesthesia. Renal and liver tissues were fixed, dehydrated, embedded, and sectioned. As previously mentioned [19 (link)], all of the specimens were sliced using an LEICA RM2245 slicing machine (Germany) to a thickness of about 4 μm, and HE was used to stain them. The biological microscope BX43 (Olympus Optical Co., Ltd., Tokyo, Japan) was used for histopathology observation, and the analysis was done with Image-Pro Plus software.
Li B., Chen X.F., Wu H.S., Su J., Ding Y.Y., Zhang Z.H., Rong M., Dong Y.J., He X., Li L.Z., Lv G.Y, & Chen S.H. (2024). The anti-hyperlipidemia effect of Atractylodes macrocephala Rhizome increased HDL via reverse cholesterol transfer. Heliyon, 10(7), e28019.
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In Vitro Capillary Formation Assay

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Matrigel (BD Biosciences) was diluted at a 1:1 ratio with cold endothelial cell growth medium (EGM-2), and the mixture was added to 24-well plates. To examine the generation of capillary-like structures in vitro [30 (link)], HUVECs were seeded in Matrigel at a density of 8 × 104 cells per well. Following cell transformation and attachment to the wall, the culture medium was removed, and different types of supernatant were added, followed by incubation at 37°C for 6 h. The length of the vessels or the number of rings per well formed by HUVECs was calculated. The structure of the capillaries was observed using an Olympus microscope (Olympus Corporation) and statistically analyzed using Image-Pro Plus software.
Wang W., Wu J., Dai X, & Cheng K. (2022). Inhibitory effect of CC chemokine ligand 23 (CCL23)/ transcription factor activating enhancer binding protein 4 (TFAP4) on cell proliferation, invasion and angiogenesis in hepatocellular carcinoma. Bioengineered, 13(1), 1626-1636.
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Rat Kidney Fibrosis Analysis

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The right kidney tissue of the rat was taken and placed in 10% formalin solution for fixation. HE staining and Masson staining were routinely performed after alcohol gradient dehydration, xylene transparency, embedding, sectioning, and gradient deparaffinization. The operation process was carried out in strict accordance with the reagent instructions. The kidney tissue damage and fibrosis were observed under a light microscope (CX41, OLYMPUS), and Image-Pro Plus software was used to semi-quantitatively analyze the degree of renal interstitial fibrosis in rats. The evaluation method is as follows: each slice was randomly selected from 5 fields under a 200x optical microscope, and the percentage of the positive staining area in the entire field of view was calculated, and the average value was taken as the positive staining area of the specimen. All measurements were performed under the same optical conditions.
Pei J., Wu M., Cai S., Peng J., Zhan X., Wang D., Wang W, & An N. (2022). The Protective Effect of Ursolic Acid on Unilateral Ureteral Obstruction in Rats by Activating the Nrf2/HO-1 Antioxidant Signaling Pathway. Computational Intelligence and Neuroscience, 2022, 3690524.
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6

Quantifying Angiogenic Potential of HUVECs

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To assess angiogenic potential, HUVECs at 1 × 10^5 cells/well were placed on 24-well plates pre-coated with 200 μl Matrigel (BD Biosciences, USA). Capillary-like structures were examined under an inverted microscope (Olympus BX-UCB; Olympus, Melville, NY), and the number of tube-like structures was quantified using Image-Pro Plus software.
Hei B., Liu R.E, & Li M. (2024). Ursolic acid inhibits glioblastoma through suppressing TGFβ-mediated epithelial-mesenchymal transition (EMT) and angiogenesis. Heliyon, 10(6), e27722.
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7

Quantifying Aortic Plaque Microvasculature

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Paraffin sections of dehydrated aortic roots were repaired with EDTA antigen retrieval solution (dilution 1:50) for 3 min, after being washed with PBS, incubated with 3% H2O2, then washed with PBS and drop 5% BSA blocking solution. The primary antibody solution was added (CD34 was diluted at 1:100, Ki67 diluted at 1:250. The NC group was replaced with 0.01 M, Ph = 7.4 PBS), and allowed to incubate overnight at 4 °C. On the second day, wash the slide with PBS, then add the secondary antibody (diluted 1:600). After incubating for 1 h, wash the slide with PBS. Finally, slide with Fluoroshield Mounting Medium with DAPI.
The images were observed and collected under a fluorescence upright microscope (Olympus, BX-51, Japan) and analyzed with Image-Pro Plus software (version 6.0). Randomly select 5 visual fields (400×) in the plaque area to measure the plaque area (PA, mm2), use CD34 as an endothelial cell marker and Ki67 as a proliferative cell marker to detect the number of VV (Q, n) in the plaque area. According to the formula microvascular density (MVD) = Q/PA, calculate the density of VV (n/mm2) (Passaro et al. 2017 (link)), and take the average value as the density of the VV of the specimen.
Qi Z., Yan Z., Zhu K., Wang Y., Fan Y., Li T, & Zhang J. (2023). Novel treatment from a botanical formulation Si-Miao-Yong-an decoction inhibits vasa vasorum angiogenesis and stabilizes atherosclerosis plaques via the Wnt1/β-catenin signalling pathway. Pharmaceutical Biology, 61(1), 1364-1373.
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8

Quantifying Neuroinflammation and Myelin Integrity in Brain Slices

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The immunofluorescence (IF) staining procedure was performed on the paraffin slices. The paraffin portions were first dewaxed and citrate‐repaired, then the brain slices were blocked for an hour at room temperature (RT) using a PBS solution containing 0.3% Triton X‐100 and 5% BSA. Subsequently, sections were allowed to incubate overnight with primary antibodies (MBP (1:1000, ABclonal, A11162); Neun (1:500, Abcam, Ab177487); Iba‐1 (1:500, Wako, 019‐19741)) at 4°C. After PBS washing, the secondary antibody (1:500, Invitrogen, A‐110081, A‐110121) was added for 1 h. Then the tissue sections were rinsed for 5 min with DAPI solution. As a final step, the sections were covered with coverslips by utilizing an antifade mounting medium to minimize fluorescence quenching. The obtained images were analyzed using Image‐Pro Plus software to quantify the number of microglia and neurons as well as the IntDen of the MBP staining after imaging by a fluorescent microscope (Olympus Corporation, Japan).
Gao X., Chen J., Yin G., Liu Y., Gu Z., Sun R., Sun X., Jiao X., Wang L., Wang N., Zhang Y., Kan Y., Bi X, & Du B. (2024). Hyperforin ameliorates neuroinflammation and white matter lesions by regulating microglial VEGFR2 /SRC pathway in vascular cognitive impairment mice. CNS Neuroscience & Therapeutics, 30(3), e14666.
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9

Quantifying Apoptosis in Skeletal Muscle

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Apoptotic nuclei in skeletal muscle were examined using double-fluorescent labeling of TUNEL and dystrophin. TUNEL staining was performed according to the manufacturer’s protocol (Roche Inc., Basel, Switzerland). After TUNEL labeling, tissue sections were incubated with a rabbit anti-dystrophin monoclonal antibody (1:200, Cat. 12715-1-AP, Proteintech) followed by an anti-rabbit IgG cyanin 3 (Cy3) (1:200, Cat. SA00009-2, Proteintech). Sections were incubated with DAPI (Meilunbio, Dalian, China) to stain nuclei and examined under a fluorescence microscope (Olympus, Tokyo, Japan). Photomicrographs were merged and saved by Image-Pro Plus software (Olympus). The numbers of TUNEL- and DAPI-positive nuclei were counted and only labeled nuclei that colocalized with dystrophin staining were counted. The data are expressed as the TUNEL index, which was calculated by counting the number of TUNEL-positive nuclei divided by the total number of nuclei. The TUNEL index for each muscle was calculated from five random, nonoverlapping fields at an objective magnification of 40×.
Jiang X., Yang J., Liu F., Tao J., Xu J, & Zhang M. (2022). Embryonic stem cell-derived mesenchymal stem cells alleviate skeletal muscle injury induced by acute compartment syndrome. Stem Cell Research & Therapy, 13, 313.
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10

Histological Analysis of Liver Granuloma and Fibrosis

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Liver tissues were fixed in 4% formalin and embedded in paraffin. Then, these histological sections were cut at 5 μm. Hematoxylin-eosin (HE) staining was used to analyse the area of granuloma. Masson staining was used to analyse the degree of collagen deposition. Images of six random microscopic fields of blue-stained collagen fibres in the liver section of each mouse were recorded using an inverted microscope (Olympus, Olympus DP27, Japan) and then digitized and analysed on Image-Pro Plus software 6.0 as previously described. A Masson Stain Kit was offered by Wuhan Servicebio Technology Company (BA-4079A, BASO, Zhuhai, China).
Duan M., Yang Y., Peng S., Liu X., Zhong J., Guo Y., Lu M., Nie H., Ren B., Zhang X, & Liu L. (2019). C/EBP Homologous Protein (CHOP) Activates Macrophages and Promotes Liver Fibrosis in Schistosoma japonicum-Infected Mice. Journal of Immunology Research, 2019, 5148575.
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