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Tissue microarray

Manufactured by Shanghai Outdo Biotech Co.
Sourced in China

Tissue microarray is a laboratory tool used for high-throughput analysis of tissue samples. It allows for the simultaneous examination of multiple tissue samples on a single slide, facilitating efficient evaluation and comparison of tissue characteristics.

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68 protocols using tissue microarray

1

Immunohistochemical Analysis of LRRFIP1 in Pancreatic Cancer

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A tissue microarray containing pancreatic cancer (n = 10) and paired adjacent normal tissues was purchased from Shanghai Outdo Biotech Company (Shanghai, China). The tissue microarray was processed and incubated with an anti-LRRFIP1 antibody (SANTA CRUZ, #SC-101168), followed by secondary antibody incubation. 3, 3'-diaminobenzidine (DAB) was used to detect the signal, and hematoxylin was used to counterstain the nuclei. The intensity of the staining was graded as 0 (negative), 1 (weak), 2 (moderate), and 3 (strong). The percentage of positively stained cells was divided into four categories: 0 (negative), 1 (1–25% positive cells), 2 (26–50% positive cells), 3 (51–75% positive cells), and 4 (> 75% positive cells) [14 (link)]. The immunohistochemistry score was calculated by multiplying the staining intensity by the percentage of positive tumor cells.
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2

Bladder Cancer Tissue Microarray Study

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The present study recruited 64 patients with BC undergoing cystectomy at Ruijin Hospital (Shanghai, China) between January, 2007 and February, 2022 (Ruijin Cohort). The present study was performed in accordance with the Declaration of Helsinki. The studies involving human participants were reviewed and approved by the Ethics Committee of Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University (PA23030202). Informed consent was obtained from each patient. The patient specimens were pathologically diagnosed using three independent experts. The BC samples and matched normal bladder mucosae were embedded into a tissue microarray (Shanghai Outdo Biotech Co., Ltd.). Clinical and pathological data were recorded, including sample ID, age, sex, operation data, follow-up period, status, tumor grade, tumor size, metastatic lesion and lymphatic infiltration, followed by determining their stages according to the 8th AJCC staging system (18 (link)).
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3

Colorectal Cancer Cell Lines and Tissue Microarray

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The HCT116 and SW480 CRC cell lines were obtained from ATCC and maintained in high-glucose DMEM supplemented with 10% fetal calf serum (FCS, HyColony, Logan, UT). A tissue microarray involving the clinicopathological information of 100 patients with CRC cancer was purchased from Shanghai Outdo Biotech Company & National Engineering Center for Biochip Design and Engineering, China.
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4

Immunohistochemistry of NLGN3 in Glioma

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A tissue microarray containing 38 glioma tissues and three normal tissues was purchased from Outdo Biotech (Shanghai, China). Immunohistochemistry (IHC) was performed to detect the expression of NLGN3 according to the method previously described (Barnes et al., 1996 (link)). Five random visual fields of each sample were selected and analyzed using Image-Pro Plus 6.0 software. The results were scored according to the proportion of positive cells: 1 (∼1–25%), 2 (∼26–50%), 3 (∼51–85%), and 4 (>85%). One was considered negative and two through four were considered positive.
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5

IHC Analysis of ALKBH5 in Gastric Cancer

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A tissue microarray containing the slides of 90 GC tumor tissues and adjacent normal tissue was purchased from Outdo Biotech with ethical approval (Shanghai, China; HStmA180Su11). The protein level of ALKBH5 was determined by a semiquantitative IHC assay, using the anti-ALKBH5 antibody (Abcam, ab244296). The results of IHC were independently given stained scores by two independent observers. The criteria are as follows: 1) ≤25% of positively stained cells; 2) 25%–50% of positively stained cells; 3) 50%–75% of positively stained cells; and 4) ≥75% of positively stained cells.
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6

Breast Cancer Tissue Microarray Analysis

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The study protocol was approved by the Ethics Committee of Shanghai Jiao Tong University School of Medicine. Tissue microarray containing 161 formalin-fixed, paraffin-embedded breast cancer tissues and 132 paired adjacent tissues was purchased from the Shanghai Outdo Biotech Company (Shanghai, China). All the tissue samples were collected with signed informed consent according to the internal review. Each core of representative areas from tumor and normal breast tissue (2.0 mm in diameter and 4 μm in thickness) were prepared according to a standard method. Clinicopathological stage and grade were assigned according to the criteria from the Union for International Cancer Control and the World Health Organization. Immunohistochemistry was performed according to the methods described in the 'Supplementary material'. DCTPP1 expression was evaluated independently by two pathologists in a blinded manner using the Allred scoring system.62 For each section, five fields were randomly selected. The staining was scored according to the percentage of DCTPP1-positive cells. For data analysis, scores >50 were considered as high expression.
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7

Lung Cancer Cell Lines and NSCLC Tissue Analysis

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The lung cancer cell lines used in this study (except for CRL-5810, CRL-5844 and CRL-5844) are kind gifts from Professor Axel Ullrich (Martinsried, Germany). The human lung cancer cells were maintained in RPMI 1640 from Invitrogen. All the other cells used in this study were cultured in DMEM from Invitrogen. Culture media were supplemented with 10% fetal bovine serum (FBS) from Invitrogen.
A tissue microarray for the immunostaining analysis of PHF14 protein expression was purchased from Shanghai Outdo Biotech (China). The array consisted of 71 paired NSCLC cancer and matched non-cancer tissues and four normal lung tissues. For qPCR analysis, 24 paired NSCLC samples were collected from Fudan University Shanghai Cancer Center. For western blot analysis, 24 paired NSCLC samples were obtained from Changhai Hospital affiliated to the Second Military Medical University, additional 20 paired NSCLC samples were obtained from Chest Hospital affiliated to Shanghai Jiaotong University. The access to human tissues complied with both Chinese laws and the guidelines of the local ethics committee. All patients participating in this study gave informed consent prior to surgery and data collection.
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8

Quantifying circDNAJC11 Expression in Breast Cancer

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A digoxin-labeled probe (Digoxin-5’- TTCAGCTCTTCAGAAGAGGCCTTGGGAT TGGTTCGCTGCT-3’-Di-goxin) was synthesized for evaluating the circDNAJC11 expression in a tissue microarray (Outdo Biotech, Shanghai, China) containing 269 BC tissues and 134 paracarcinoma tissues. The tissue microarray was dewaxed, rehydrated, digested with proteinase K, and hybridized with the circDNAJC11 probe at 45 °C for 13 h. Afterward, the issues were combined with a biotin-conjugated anti-digoxin antibody for incubation overnight at 4℃, followed by 3,3-diaminobenzidine (DAB) staining. CircDNAJC11 expression was quantified by multiplying the positive staining intensity score (strong = 3, medium = 2, weak = 1, and negative = 0) by the percentage of positive-stained cells (> 76% = 4, 51–75% = 3, 26–50% = 2, 5–25% = 1, and < 5% = 0).
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9

Exploring MYH9 Expression in CRC

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Clinical CRC samples and paired normal tissues used in the exploration of MYH9 expression by western blot were supplied by tissue bank of Department of General Surgery, Nanfang Hospital (Guangzhou, China). Tissue microarray, including 100 human CRC samples and 80 normal ones, was purchased from Outdo Biotech (Shanghai, China) for immunohistochemistry (IHC) analysis.
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10

MRPL15 Expression in Lung Cancer

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The tissue microarray containing 92 lung cancer tissues and 88 adjacent normal tissues was purchased from Shanghai Outdo Biotech (Shanghai, China). IHC staining was performed as previously described (32 (link)) with mouse monoclonal MRPL15 antibody (OriGene Technologies Inc., TA807480, 1:300 dilution). The staining results were identified by integrated scoring: the immunostaining intensity (negative, no staining of cells = 0; weak staining = 1; moderate staining = 2; strong staining = 3) and the percentage of the positively stained area (0–25% = 1, 26–50% = 2, 51–75% = 3, >75% = 4). The score was finally calculated according to the above staining criteria, and the scores over 6 were regarded as high expression.
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