To compare the lymphatic transport between DiR-modified liposomes and ICG (Diagnogreen; Daiichi Sankyo Company, Ltd., Tokyo, Japan), 0.02 ml of either DiR-modified liposomes or ICG (2.5 mg/ml) was injected into the footpad of each PLN-resected mouse, and time-dependent lymphatic transport was observed by the PDE.
Diagnogreen
Manufactured by Daiichi Sankyo
Sourced in Japan
Diagnogreen is a laboratory equipment product designed for use in diagnostic and research settings. It is a compact and versatile device that serves as a platform for various analytical and detection applications. The core function of Diagnogreen is to facilitate accurate and efficient measurement and analysis of samples, supporting the work of researchers and laboratory professionals.
Automatically generated - may contain errors
23 protocols using diagnogreen
1
Lymphatic Transport of DiR-Liposomes vs. ICG
2
Visualizing Blood Vessels with ICG and Tomato Lectin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pharmaceutical grade ICG (Diagnogreen) was purchased from Daiichi-Sankyo (Tokyo, Japan). Blood vessels were visualized using Lycopersicon esculentum (tomato) lectin conjugated with DyLight 488 (TL488; Vector Laboratories, Burlingame, CA, USA).
3
Intraoperative Tumor Visualization Using ICG
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 12–24 h before surgery, ICG (Diagnogreen; Daiichi Sankyo Co. Ltd., Tokyo, Japan) was diluted to 5 mg/mL with distilled water for injection, and 2.0 mg/kg was administered intravenously. During surgery, pulmonary masses were observed visually in the lobes of the lungs exposed by thoracotomy. For the confirmed pulmonary masses, the lung surface was observed with an infrared camera system (HyperEye Medical System; Mizuho Medical Co. Ltd, Tokyo, Japan) before surgical resection (Figure 1 ). When images were taken, the surgical light was turned off and images were taken at a distance of 30–50 cm from the lung surface. Regardless of the fluorescent findings, pulmonary masses and lymph nodes, for which surgical resection was indicated, were removed. The resection margins of the pulmonary masses in the body were also imaged under the same conditions. The excised masses and lymph nodes were imaged under the same conditions in a shaded box, and the fluorescence was compared.
The fluorescence intensity was defined as 1 when it was stronger than the surrounding normal lung tissue, 0 when it was equivalent, and −1 when it lacked fluorescence. A score of 1 was defined as “fluorescent”, while 0 and −1 were defined as “no fluorescence”.
The fluorescence intensity was defined as 1 when it was stronger than the surrounding normal lung tissue, 0 when it was equivalent, and −1 when it lacked fluorescence. A score of 1 was defined as “fluorescent”, while 0 and −1 were defined as “no fluorescence”.
4
Laparoscopic Sentinel Node Mapping in Gastric Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Laparoscopic sentinel basin dissection SNB was described in detail in a previous report 10 . To detect the sentinel nodes, tracers were prepared with a mixture of 2⋅5 mg/ml concentrated indocyanine green (2 ml, 5 mg Diagnogreen ® ; Daiichi-Sankyo, Tokyo, Japan) and 99m Tc-radiolabelled human serum albumin (2 ml, 0⋅1 mCi/ml). The National Cancer Centre prepared uniform tracer and delivered it to each participating institution. During surgery, 1 ml dual tracer was injected endoscopically into the submucosal layer in four quadrants of the gastric tumour. Fifteen minutes after injection, sentinel basins containing sentinel nodes were detected by gross and laparoscopic γ detectors. Sentinel nodes were dissected and divided into four groups: hot nodes (radioactive nodes, 10 times or more radioactivity detected by γ detector compared with surrounding surgical field); green nodes (indocyanine green-stained nodes); both hot and green nodes; and basin nodes (nodes within sentinel basins that were neither hot nor green). All harvested sentinel nodes were sent to the pathologist for intraoperative frozen-section analysis.
5
Intraoperative Imaging of Hepatic Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In 9 patients who underwent hepatic resection for malignancies (10 nodules: HCC, n = 5; CRLM, n = 4; and ICC, n = 1), ICG (Diagnogreen, Daiichi Sankyo, Tokyo, Japan) was preoperatively injected (dose, 0.5 mg per kg of body weight; intravenous injection) as part of routine liver function testing performed for surgical planning [12] (link), [17] (link). Intraoperatively, the ICG retained in hepatic tissues was utilized as a contrast agent in PA tomography as well as for fluorescence imaging. Following hepatectomy, PA images of the resected specimens were obtained, and PA signal amplitude was measured with the Vevo LAZR imaging system in the following regions of interest (ROI): cancerous tissue (CA); non-cancerous hepatic tissue around the tumors (Peri), and non-cancerous hepatic tissue 2 mm from the tumors (NC). The localization of ICG on the resected specimens' cut surfaces was also evaluated by macroscopic fluorescence imaging with a Maestro imaging system followed by pathological examination with fluorescence microscopy [17] (link).
6
Preoperative Liver Function Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before surgery, each patient underwent conventional liver function tests, and measurement of ICGR15. The fluorescent source was ICG (Diagnogreen; Daiichi Sankyo, Tokyo, Japan), which was injected at a dose of 0.5 mg/kg i.v. at 1–8 weeks prior to surgery. Hepatitis screening was done by measurement of hepatitis B surface antigen and hepatitis C antibody. The levels of α-fetoprotein and PIVKA-II (protein induced by vitamin K absence/antagonism-II) were also measured in all patients. Surgical procedures were classified according to the Brisbane terminology proposed by Strasberg et al. [13 ]. One senior pathologist reviewed each specimen for histological confirmation of the diagnosis.
7
Lymphatic Vessel Visualization with ICG Injection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ICG, 25 mg (Diagnogreen; Daiichi Sankyo, Tokyo, Japan), was diluted with 10 mL of pure water. Injection sites were marked around the foot along the border between the dorsum and planta. A total of 19 injection sites in the foot were selected (Fig 1), and 0.05 mL of ICG solution was subcutaneously injected by using a 1-mL syringe with a 30-gauge needle. Immediately after the injections, gentle hand massage was applied at each injection site and then to the lower limb. The lymphatic vessels were identified with a near-infrared camera system (ASI224MC; Suzhou ZWO, Suzhou, China)
225
225
8
Intraoperative ICG Angiography Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tegaderm (3 M, St. Paul, MN) was used to preserve the multiple reference crosses marked on the skin until surgery. On the day of surgery, skin surface reference points were marked again after removing the film sheet. After disinfecting the skin surface with povidone-iodine, a new Tegaderm film was applied to preserve the reference crosses during surgery. The area around the abdominal flap was covered by a blue drape after abdominal flap elevation before performing ICG administration (Supplemental Digital Content 2 shows the ICG angiography settings). Two milliliters of ICG (2.5 mg/ml Diagnogreen; Daiichi Sankyo, Tokyo, Japan) was injected intravenously. ICG emission signals were detected by PDE-neo (Hamamatsu Photonics, Hamamatsu City, Shizuoka, Japan) with the following settings: distance, 25 cm; room lights, off; brightness, -1.5; contrast, 2.5; excitation light, 10.0. The arterial phase was defined as the stage in which fluorescence imaging began and spread linearly with pulsation.
9
ICG Fluorescence Angiography with Near-Infrared Endoscope
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ICG fluorescence angiography was performed using a near-infrared endoscope system (D-Light P System; Karl Storz SE & Co. KG, Tuttlingen, Germany). This device is a special camera system that can perform ICG fluorescence angiography and is usually used for laparoscopic surgery. ICG (Diagnogreen; Daiichi-Sankyo Pharmaceutical, Tokyo, Japan) was intravenously injected at 0.2 mg/kg body weight and became instantly bound to globulins in the plasma. It is not metabolized in the body; it is excreted unchanged exclusively by the liver and has a plasma half-life of three to four minutes.17 (link) ICG becomes fluorescent once excited by near-infrared light at wavelengths of about ⩾ 820 nm; the absorption peak is around 807 nm, and the emission peak is around 822 nm.13 (link) ICG fluorescence angiography can provide differentiation of vital anatomic structures from a few millimetres to more than 1 cm.18 (link)
10
Lymphatic Imaging of Roxadustat Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lymphatic structures in the hindlimbs of the control and roxadustat groups were compared by fluorescent lymphography every week for 4 weeks postoperatively (n = 6 per group). Prior to imaging, isoflurane was used to anesthetize the mice and their residual fur was removed. A 5-μL volume of indocyanine green solution (2.5 mg/mL Diagnogreen in distilled water; Daiichi Sankyo Company, Ltd., Tokyo, Japan;) was subcutaneously injected into both paws with a 26-gauge needle. Imaging was performed indocyanine green using a near-infrared fluorescence camera system (Photodynamic Eye; Hamamatsu Photonics, Hamamatsu, Japan) 15 min after indocyanine green injection. The coverage of the fluorescent area (i.e., the dermal backflow at the thigh) was measured using ImageJ software (National Institutes of Health).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!