1290 infinity series uhplc system

Manufactured by Agilent Technologies

Sourced in United States, Germany

The 1290 Infinity series UHPLC System is a high-performance liquid chromatography (HPLC) system designed for ultra-high-pressure liquid chromatography (UHPLC) applications. It is capable of operating at pressures up to 1,300 bar (19,000 psi) and features advanced fluidic and detection technologies to enable rapid, high-resolution separations with increased sensitivity and speed.

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Lab products found in correlation

Uplc beh amide column, by Waters Corporation (5 mentions) Tripletof 6600 mass spectrometry, by AB Sciex (5 mentions) Analyst tf 1, by AB Sciex (4 mentions) Tripletof 6600 mass spectrometer, by AB Sciex (2 mentions) Kinetex c18 column, by Phenomenex (2 mentions) 6490 series esi triple quadrupole mass spectrometer, by Agilent Technologies (2 mentions) Jetstream source, by Agilent Technologies (2 mentions) Masshunter quantitative analysis software, by Agilent Technologies (2 mentions) Poroshell 120 ec c18 column, by Agilent Technologies (2 mentions) Ammonia hydroxide, by CNW Technologies (1 mentions) Analyst tf v 1, by AB Sciex (1 mentions) Ammonium acetate, by CNW Technologies (1 mentions) Beh amide column, by Waters Corporation (1 mentions) Sciex 5500 qtrap mass spectrometer, by AB Sciex (1 mentions) Kinetex evo c18 column, by Phenomenex (1 mentions) δ9 thc, by Lipomed (1 mentions) Api 4000, by AB Sciex (1 mentions) Agilent 6120 quadrupole mass spectrometer, by Agilent Technologies (1 mentions) Zorbax eclipse plus c18, by Agilent Technologies (1 mentions) Lambda 35, by PerkinElmer (1 mentions)

Close spelling variants of this product

1290 Infinity II series UHPLC System Agilent 1290 Infinity series UHPLC System Agilent 1290 Infinity II series UHPLC System UHPLC 1290 Infinity-Series 1290 Infinity UHPLC system 1290 series UHPLC system 1290 Infinity UHPLC 1290 series UHPLC 1290 Infinity Series 1290 UHPLC system

21 protocols using 1290 infinity series uhplc system

UHPLC-MS/MS Analysis of Metabolites

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The UHPLC separation was conducted using a 1,290 Infinity series UHPLC System (Agilent Technologies, MA, United States) coupled to a UPLC BEH amide column (1.7 µm, 2.1 × 100 mm; Waters, Milford, United States). The mobile phase was composed of ammonium acetate (25 mmol/L, CNW Technologies, Shanghai, China) and ammonia hydroxide (25 mmol/L, CNW Technologies), which were added to water (pH = 9.75) (A) and acetonitrile (B), respectively. The following elution gradient was used for analysis: 0–0.5 min, 95% B; 0.5–7.0 min, 95%–65% B; 7.0–8.0 min, 65%–40% B; 8.0–9.0 min, 40% B; 9.0–9.1 min, 40%–95% B, and 9.1–12.0 min, 95% B. The injection volume, auto-sampler temperature, and column temperature were 2 µl (both positive and negative modes), 4°C, and 25°C, respectively (19 (link)).
MS/MS data acquisition and analysis were conducted using the Analyst TF v1.7 (AB Sciex, MA, United States) according to a preset standard. In each cycle, a collision energy (CE) of 30 eV and cycle time of 0.56 s was used to select the densest 12 precursor ions with an intensity >100 for MS/MS. The following were set as the electrospray ionization (ESI) source conditions: curtain gas (35 psi), gas 1 (60 psi), gas 2 (60 psi), ion spray voltage floating (5,000 V in the positive mode and −4,000 V in the negative mode), declustering potential (60 V), and source temperature (600°C) (19 (link)).

Tan R., Ou S., Kang T., Wu W., Xiong L., Zhu T, & Zhang L. (2023). Altered serum metabolome associated with vascular calcification developed from CKD and the critical pathways. Frontiers in Cardiovascular Medicine, 10, 1114528.

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Metabolite Analysis of A. contorta

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Roots, stems, leaves, and fruits of A. contorta were used for metabolite analysis on an LC/MS system. The UHPLC separation was performed using a 1290 Infinity series UHPLC System (Agilent Technologies). MS/MS spectra were obtained using the TripleTOF 6600 mass spectrometry system (AB Sciex) on an information-dependent basis (IDA). Analyst TF 1.7 (AB Sciex), ProteoWizard, and XCMS [125 (link)] software were used for data analysis.

Cui X., Meng F., Pan X., Qiu X., Zhang S., Li C, & Lu S. (2022). Chromosome-level genome assembly of Aristolochia contorta provides insights into the biosynthesis of benzylisoquinoline alkaloids and aristolochic acids. Horticulture Research, 9, uhac005.

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UHPLC-MS/MS for Metabolite Profiling

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UHPLC separation was performed using a 1290 Infinity series UHPLC System (Agilent Technologies) with a UPLC BEH amide column (2.1*100 mm, 1.7 μm, Waters). The column temperature was maintained at 25°C. The mobile phase was A, containing 25 mmol/L ammonium acetate and 25 mmol/L ammonia hydroxide (pH=9.75) in water, and B was acetonitrile. The gradient conditions were as follows: 95% B at 0–0.5 min, 95–65% B at 0.5–7.0 min, 65–40% B at 7.0–8.0 min, 40% B at 8.0–9.0 min, 40–95% B at 9.0–9.1 min, and 95% B at 9.1–12.0 min. The flow rate of the mobile phase was 0.5 L/min, and the autosampler temperature was set at 4°C. The injection volume was 1 μL for both negative mode and positive mode.
During the LC/MS experiment, TripleTOF 6600 mass spectrometry (AB Sciex) and information-dependent acquisition (IDA) were adapted to acquire MS/MS spectra. In IDA mode, Analyst TF (version 1.7, AB Sciex), the acquisition software, evaluated the full scan survey MS data and collected the MS/MS spectra depending on the preselected criteria. The 12 most intensive precursor ions with intensities greater than 100 were selected for MS/MS in each cycle; the collision energy was 30 eV, and the cycle time was 0.56 s. The ESI source parameters were set as follows: gas 1:60 psi; gas 2: 60 psi; CUR: 35 psi; TEM:600°C; DP: 60 V; ISVF: 5000 V in positive mode and −4000 V in negative mode.

Pan X., Chen W., Nie M., Liu Y., Xiao Z., Zhang Y., Zhang W, & Zou X. (2021). A Serum Metabolomic Study Reveals Changes in Metabolites During the Treatment of Lung Cancer-Bearing Mice with Anlotinib. Cancer Management and Research, 13, 6055-6063.

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UHPLC Quantification of Epicatechin and Catechin in Dark Chocolate

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Quantification of epicatechin and catechin in dark chocolate samples was performed according to Coklar and Akbulut (2017) and Demir et al.,. (2014) with some modifications. For that, an Agilent 1290 Infinity Series UHPLC system equipped with a G7167B mutisampler, G7104A flexible pump, G7116B column oven, and a G7117B diode array detector was used. Before the injection, the extracts were filtered through a 0.45 μm pore size x 33 mm syringe filter (Merck, Millex, Germany). The separation was achieved using a reversed-phase C18 column (5 μm, 250 × 4.6 mm i.d.). The mobile phase was (A) water/acetic acid (98:2) and (B) water/acetonitrile/acetic acid (78:20:2). The flow rate was 0.75 mL/min and the gradient was as follows: 10–14% B (5 min), 14–23% B (11 min), 23–35% B (5 min), 35–40% B (14 min), 40–100% B (3 min), 100% B isocratic (3 min), 100–10% B (3 min) and 10% B isocratic (4 min). The detector was set to 280 nm. The column temperature was 40 °C. The epicatechin and catechin were quantified by comparison with peak areas of each standard. The data was analyzed using the Chemstation software.

Medina-Mendoza M., Rodriguez-Pérez R.J., Rojas-Ocampo E., Torrejón-Valqui L., Fernández-Jeri A.B., Idrogo-Vásquez G., Cayo-Colca I.S, & Castro-Alayo E.M. (2021). Rheological, bioactive properties and sensory preferences of dark chocolates with partial incorporation of Sacha Inchi (Plukenetia volubilis L.) oil. Heliyon, 7(2), e06154.

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Metabolomics Profiling Using UHPLC-MS/MS

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The UHPLC-MS/MS analyses were applied to the 1290 Infinity series UHPLC System (Agilent Technologies) coupled with a TripleTOF 6600 mass spectrometer (AB Sciex) from Biomarker Technologies (Beijing, China). The MS raw data were converted to the mzXML format by ProteoWizard, and processed by R package XCMS (version 3.2). This process included peak deconvolution, alignment and integration. Minfrac and cut off were set as 0.5 and 0.3, respectively. An in-house MS/MS database was applied for metabolites identification. The principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed using R. We employed a univariate analysis to calculate statistical significance; metabolites with VIP ≥ 1, fold change ≥ 2.0 or ≤ 0.50, and a p-value <0.05, were considered differentially expressed metabolites (DEM). Volcano plots were used to filter the metabolites of interest based on the log2 (FC) and log10 (p-value) of the metabolites. The KEGG database was used to annotate the differentially expressed metabolites with a p-value less than 0.05 set as the threshold. The enrichment factor represented the ratio between the proportion of differentially expressed metabolites in the pathway and all the metabolites in the pathway; the greater the value, the greater the degree of enrichment.

Liu C., Pan J., Yin Z.G., Feng T., Zhao J., Dong X, & Zhou Y. (2022). Integrated transcriptome and metabolome analyses revealed regulatory mechanisms of flavonoid biosynthesis in Radix Ardisia. PeerJ, 10, e13670.

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Untargeted Metabolomics Extraction Protocol

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Samples (25 mg) were added to EP tubes containing 1,000 μL extract solution (acetonitrile : methanol : water, 2 : 2 : 1) and the internal standard, L-2-chlorophenylalanine (2 μg/mL). The samples were Vortex-mixed, homogenized, sonicated in an ice-water bath, then incubated and centrifuged at 10000 rpm for 15 min at 4°C. Supernatants were decanted into fresh tubes, dried in vacuo. Then, the dried samples were reconstituted in 50% acetonitrile. The constitution was then centrifuged at 13000 rpm for 15 min at 4°C, and supernatant was transferred to a fresh glass vial for LC/MS analysis. The UHPLC separation proceeded using a 1290 Infinity series UHPLC System (Agilent Technologies Inc., Santa Clara, CA. USA), equipped with a UPLC 2.1 × 100 mm, 1.7 μm, BEH Amide column (Waters Corp., Milford, MA, USA). The autosampler injection was divided into the positive and negative ion modes. We acquired MS/MS spectra on an information-dependent basis (IDA) during LC/MS experiments using a TripleTOF 6600 mass spectrometer (AB Sciex LLC). In this mode, Analyst TF 1.7 software (AB Sciex LLC) continuously evaluates the full scan-surveyed MS data as it collects and triggers the acquisition of MS/MS spectra depending on specified criteria.

Wan S., Yang J., Mamtawla G., Zhang L., Gao X, & Wang X. (2020). Differential Metabolomic Analysis of Liver Tissues from Rat Models of Parenteral Nutrition-Associated Liver Disease. BioMed Research International, 2020, 9156359.

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Quantitative Analysis of Zearalenone Analogues

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ZEN, HZEN, and DHZEN in digesta were analyzed on a 1290 Infinity series UHPLC system (Agilent Technologies, Waldbronn, Germany) coupled to a Sciex 5500 QTRAP mass spectrometer (Sciex, Foster City, CA, USA). Chromatographic separation was performed on a Kinetex EVO C18 column (150 mm × 2.1 mm, 2.6 µm, Phenomenex, Aschaffenburg, Germany). Eluents A and B consisted of water/ACN/HAc (A = 94.9/5.0/0.1, vol/vol/vol; B = 4.9/95.0/0.1, v/v/v). The total run time was 3.95 min. The gradient started at 38% B, which was held for 0.2 min. Thereafter, the proportion of B was linearly increased to 55% at 2.6 min, and to 100% at 2.7 min. Subsequently, 100% B was held until 3.4 min. Finally, the column was re-equilibrated at 38% B for 0.45 min. Injection volume, flow rate and column temperature were 1 µL, 0.65 mL/min and 30 °C, respectively. Mass spectrometric detection was carried out in negative electrospray ionization mode with multiple reaction monitoring as scan type. The following source settings were applied: ion spray voltage −4500 V, source temperature 500 °C, curtain gas 35 psi, ion source gas 1 at 50 psi, and ion source gas 2 at 50 psi. LOQs for ZEN, HZEN, and DHZEN were 28 ng/g (88 nmol/kg), 28 ng/g (83 nmol/kg), and 70 ng/g (240 nmol/kg), respectively.

Gruber-Dorninger C., Killinger M., Höbartner-Gußl A., Rosen R., Doupovec B., Aleschko M., Schwartz-Zimmermann H., Greitbauer O., Marković Z., Stanković M., Schöndorfer K., Vukmirovic D., Wein S, & Schatzmayr D. (2023). Enzymatic Degradation of Zearalenone in the Gastrointestinal Tract of Pigs, Chickens, and Rainbow Trout. Toxins, 15(1), 48.

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Metabolomic Analysis of Sow Milk

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Milk samples randomly selected from six sows in each group were used for extraction and sent for metabolomic analysis (Shanghai Biotree Biotech Co., Ltd., Shanghai, China). An equivalent volume was aliquoted from each sample, mixed to prepare the quality control sample and dried in a vacuum concentrator. In addition, methoxymethyl amine salt was mixed with the dried samples, and bis(trimethylsilyl)trifluoroacetamide was added. After the samples cooled to room temperature, fatty acid methyl ester was added to each sample and mixed. Ultra-high-performance liquid chromatography (1290 Infinity series UHPLC System, Agilent Technologies, Santa Clara, CA, USA) was applied for LC-TOF/MS analysis. A UPLC BEH amide column (internal diameter, 2.1 × 100 mm, 1.7 μm, Waters, Milford, MA, USA) was used for separation. The obtained data were applied for sparse partial least squares discriminant analysis (sPLS-DA). Differential metabolites were identified with variable importance projection (VIP) > 1.0 and P < 0.05. To further interpret the biological significance of metabolites, metabolic pathway analyses were performed by an online analysis platform in MetaboAnalyst 5.0 (https://www.metaboanalyst.ca/). KEGG analysis was conducted using the enrichment analysis sections of MetaboAnalyst.

Wang C., Wei S., Liu B., Wang F., Lu Z., Jin M, & Wang Y. (2022). Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota. Gut Microbes, 14(1), 2057779.

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Quantification of Cannabinoids by LC-MS/MS

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A total of eight tissue-free standards were prepared to obtain a calibration curve for Δ9-THC, OH-THC (Lipomed, Arlesheim, Switzerland), COOH-THC (Lipomed, Arlesheim, Switzerland) and CBD (Trigal Pharm, Vienna, Austria), with concentrations in the range of 1–500 ng/mL. Quantification of cannabinoids in tissue samples was performed by LC-MS/MS using a 1290 Infinity series UHPLC System (1290 Infinity series, Agilent, Germany) coupled to a triple quadrupole mass spectrometer (API 4000^®, Sciex, Framingham, MA, USA). LC separation was achieved on a Nucleodur C18 Isis reverse-phase column (50 × 2.0 mm, 1.8 μm; Macherey-Nagel, Germany). The mobile phase system was 0.05% acetic acid in water (eluent A) and acetonitrile (eluent B) at a flow rate of 0.6 mL/min and 50 °C. Detection was performed in the positive electrospray ionization (ESI) mode applying the following detection parameters: curtain gas, 30; CAD gas, 6; gas 1, 50; gas 2, 60; ion spray voltage, 4500 V; source temperature, 500 °C. The LC-MS/MS system was operated with the Software Analyst^® Version 1.7.1 (Sciex, Framingham, MA, USA). Transitions were recorded using the multiple reaction monitoring (MRM mode, Table 1).

Dumbraveanu C., Strommer K., Wonnemann M., Choconta J.L., Neumann A., Kress M., Kalpachidou T, & Kummer K.K. (2023). Pharmacokinetics of Orally Applied Cannabinoids and Medical Marijuana Extracts in Mouse Nervous Tissue and Plasma: Relevance for Pain Treatment. Pharmaceutics, 15(3), 853.

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Non-targeted metabolomic profiling of mouse stool and serum

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Metabolites from mouse stool and serum were extracted using an acetonitrile/methanol/water (2:2:1, v/v/v) mixture. The extract was dried and reconstituted in 50% acetonitrile for instrument analysis. A pooled quality control sample was prepared and periodically analysed together with true samples. Non-targeted metabolomic analysis was performed by Biotree on a 1290 Infinity series UHPLC System (Agilent Technologies) coupled to a Triple time-of-flight (TOF) 6600 mass spectrometer (AB Sciex) equipped with an electrospray ionization source. Briefly, chromatographic separation was achieved using a Waters BEH Amide column (2.1 × 100 mm, 1.7 µm) at 25 °C. The mobile phase consisted of water with 25 mM ammonium acetate and 25 mM ammonium hydroxide (A) and acetonitrile (B). The flow rate was 0.5 ml min⁻¹. Mass spectrometry analysis was operated in information-dependent basis mode under positive and negative modes. The 12 most abundant ions were selected, fragmented and sent to a TOF mass analyser to acquire MS2 information. Data pretreatment including peak deconvolution, alignment and integration were achieved by XCMS (v.3.2) implemented in R language. Metabolite identification was based on an in-house MS2 database. MetaboAnalyst was employed for pathway analysis. Heatmaps were generated by Complex Heatmap.

Wei W., Wong C.C., Jia Z., Liu W., Liu C., Ji F., Pan Y., Wang F., Wang G., Zhao L., Chu E.S., Zhang X., Sung J.J, & Yu J. (2023). Parabacteroides distasonis uses dietary inulin to suppress NASH via its metabolite pentadecanoic acid. Nature Microbiology, 8(8), 1534-1548.

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