Ec lab

Manufactured by Bio-Logic

Sourced in France, United States

EC-Lab is a versatile electrochemical workstation designed for research and development applications. It provides users with advanced electrochemical measurement capabilities, allowing for the characterization and analysis of various electrochemical systems. The core function of EC-Lab is to enable precise control and monitoring of electrochemical experiments, facilitating the study of electrochemical processes and materials.

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Lab products found in correlation

Chi660c potentiostat, by CH Instruments (1 mentions) Sp 200, by Bio-Logic (1 mentions) Afg3251, by Tektronix (1 mentions) Sp 300, by Bio-Logic (1 mentions) Vsp 300 potentiostat, by Bio-Logic (1 mentions)

9 protocols using ec lab

Electrochemical Characterization of Anti-Aβ Biosensor

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EIS was performed using EC-Lab (SP-200, Bio-Logic, France) in 5 mM [Fe(CN)₆]^3−/4− in PBS (pH 7.4) at each step of the anti-aβ/SAM/ICE biosensor development and various time intervals of the disaggregation process of aβ_agg. The EIS data was obtained by putting on a 10 mV of sinus amplitude which satisfy the linear properties of electrical response of the object between the working and reference electrode from 100 mHz to 1 MHz of range frequency. Z-Fit software (EC-Lab, Bio-Logic, France) was used to fit the obtained EIS data through by the Randle’s equivalent circuit.
Fourier-transform infrared (FT-IR) spectra was characterized by using Jasco-4600 FTIR (USA) spectrometer to confirm the functional groups for the fabrication steps of the anti-aβ/SAM/ICE biosensor.

Le H.T, & Cho S. (2021). Deciphering the Disaggregation Mechanism of Amyloid Beta Aggregate by 4-(2-Hydroxyethyl)-1-Piperazinepropanesulfonic Acid Using Electrochemical Impedance Spectroscopy. Sensors (Basel, Switzerland), 21(3), 788.

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Solid Electrochemical Cell Mobility Measurement

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A cell filled with a powder sample (so-called “solid electrochemical cell”) was used for the calculation of the mobility of charge carriers via the Mott–Gurney law [28 (link),29 (link)]. It consisted of two flat steel electrodes (d = 5 mm). A powder sample was placed between the electrodes and fixed there with spring clamps with a constant force to a thickness of 1 mm (the mass of each material depended on its bulk density). The I–V curves (from 0 to +10 V) and PEIS data (eight potential steps at 0; 0.2; 1; 2; 3; 4; 6; and 8 V with 100 mV amplitude in the frequency range of 1–700 kHz) were registered using an SP150 potentiostat (BioLogi, Seyssinet-Pariset, France). The ZView (Scribner, New York, NY, USA) and EC-Lab (BioLogic, Seyssinet-Pariset, France) software were used for data simulation and calculations.

Shabalina A.V., Gotovtseva E.Y., Belik Y.A., Kuzmin S.M., Kharlamova T.S., Kulinich S.A., Svetlichnyi V.A, & Vodyankina O.V. (2022). Electrochemical Study of Semiconductor Properties for Bismuth Silicate-Based Photocatalysts Obtained via Hydro-/Solvothermal Approach. Materials, 15(12), 4099.

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Electrochemical Characterization of Immunosensor Fabrication

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Electrochemical impedance spectroscopy (EIS) and CV were used to monitor the electrochemical performances of immunosensor at different steps of the layer-by-layer procedure of immunosensor fabrication. The electrochemical measurements were conducted in 10 mM K₄Fe(CN)₆/K₃Fe(CN)₆ (1:1 ratio) by using a three-electrode set up including the modified Au electrode (1.5 mm² surface area) as working electrode (WE), a Pt wire as an auxiliary electrode (AE), and Ag/AgCl as the reference electrode (RE). The EIS and CV were carried out using an EC-Lab (Bio-Logic, sp-200, Seyssinet-Pariset, France) and a CHI 660C potentiostat (CH Instruments Inc., Austin, TX, USA), respectively. For EIS analysis, a bias voltage of 10 mV was applied between WE and CE over a frequency range of 0.1–1000 Hz. The generated EIS data were fitted to the Randles′ equivalent circuit model using ZView software (Solartron Analytical, Farnborough, UK).

Shiravandi A., Yari F., Tofigh N., Kazemi Ashtiani M., Shahpasand K., Ghanian M.H., Shekari F, & Faridbod F. (2022). Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker cis P-tau by a Label-Free Electrochemical Immunosensor. Biosensors, 12(10), 879.

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Electrical Characterization of Biosensor

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A custom-made chip-holder based on pogo-pins (Mill-Max Corp.) was employed to electrically connect our biosensor to all equipment (Fig. 1A).
EIS measurements were performed using a high precision impedance analyzer (Zurich Instruments MFIA) controlled by the software LabOne® or the SP-200 potentiostat controlled by the EC-Lab® software package (Bio-Logic, TN, USA). We carried out EIS measurements after serum incubation and after AuNP binding with the presence of 50 μL of 10⁻⁶ M PBS inside the PDMS well. A sinusoidal voltage with an amplitude of 10 mV and zero DC bias was applied to the IDE sensor as an input, and the impedance spectrum was measured at the frequency range from 100 Hz to 1000 kHz.
During DEP experiments, sinusoidal signals were applied to the IDEs via the chip-holder by using a function generator (Tektronix AFG 3251) at various frequencies and AC voltages.

Zeng J., Duarte P.A., Ma Y., Savchenko O., Shoute L., Khaniani Y., Babiuk S., Zhuo R., Abdelrasoul G.N., Charlton C., Kanji J.N., Babiuk L., Edward C, & Chen J. (2022). An impedimetric biosensor for COVID-19 serology test and modification of sensor performance via dielectrophoresis force. Biosensors & Bioelectronics, 213, 114476.

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Cyclic Voltammetry of GF Electrodes

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The cyclic voltammetry (CV) response of bare and modified GF was recorded using a potentiostat (EC-Lab®, Bio-Logic SP-300 electrochemical workstation). The scanning window of the experiment was −1 V to 1 V (vs. Ag/AgCl as reference electrode) and Platinum was used as an inert metal counter electrode at a scanning speed of 10 mV/s. The current responses were recorded by immersing the electrodes in H₂O₂ (0.98 M), phosphate buffer (50 mM) solution, or E. coli (2.5 × 10⁸ CFU/ml) in H₂O₂ (0.98 M) separately to measure the performance. A 5 ml of E. coli culture in LB media was centrifuged, and collected cell pellets were mixed with 5 ml DI water when used for the CV experiment. The surface area of the modified and bare GFs was determined using Brunauer Emmett Teller (BET) analysis conducted with Quantachrome instruments. The electrode morphologies were analyzed using a scanning electron microscope (SEM, SEM- EVO 18, Carl Zeiss) equipped with energy-dispersive X-ray spectroscopy (EDS, EDS-51-ADDD-0048, Oxford Instruments). For this study, a bare GF, a modified GF, and a used modified GF (after 100 uses) were observed. These electrodes were subjected to gold sputtering and images were captured at different magnifications and resolutions. The presence of different elements was confirmed using EDS.

Sharma A., Mishra A, & Chhabra M. (2024). Rapid measurement of bacterial contamination in water: A catalase responsive-electrochemical sensor. Heliyon, 10(5), e26724.

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Electrochemical Characterization of Zinc Anode

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Zn foils with a diameter of 12 mm and a thickness of 200 µm as the electrode and a piece of glass fiber (GE-Whatman) as a separator were assembled into a CR-2032 type coin cell in an open environment. 120 µL of the corresponding electrolyte were added. Electrochemical cycling tests in Zn||Zn symmetric cells, Zn||SS cells, Zn||α-MnO₂, Zn||V₂O₅, and Zn||VOPO₄ cells were recorded on a multichannel-current static system (Arbin Instruments BT 2000, College Station, TX, USA). The cyclic voltammetry (CV) and linear sweep voltammetry (LSV) were conducted on a VMP-300 electrochemical workstation (EC-lab, Biologic). The corrosion, diffusion, and hydrogen evolution behaviors of Zn foil anode were performed by a three-electrode system (Zn foil as working electrode, Pt as the counter electrode, and Ag/AgCl as reference electrode) on the VMP-300 electrochemical workstation. The corrosion Tafel plot was recorded by performing LSV with a potential range of ±0.3 V vs. open-circle potential of the system at a scan rate of 1 mV s⁻¹. The diffusion curves were measured by chronoamperometry method under an overpotential of −150 mV. The hydrogen evolution performance was collected through LSV with a potential range of −0.9~−1.6 V vs. Ag/AgCl in the 2 M Na₂SO₄ electrolyte with or without C₃N₄QD additives at a scan rate of 1 mV s⁻¹.

Zhang W., Dong M., Jiang K., Yang D., Tan X., Zhai S., Feng R., Chen N., King G., Zhang H., Zeng H., Li H., Antonietti M, & Li Z. (2022). Self-repairing interphase reconstructed in each cycle for highly reversible aqueous zinc batteries. Nature Communications, 13, 5348.

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Silicon Anode Coin Cell Assembly

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The anodes were cut into 12 mm – diameter discs and were dried under vacuum for 12 hours at 50 °C and 2 hours at 100 °C. The mass loading of the anodes was 2 mgSi cm⁻². CR2032 coin cells were assembled inside a glovebox filled with ultra-high-pure argon (content of O₂ and H₂O < 0.1 ppm). The coin cell contained a silicon anode, a 2400 Celgard separator (19 mm diameter) and a lithium-foil counter electrode (15 mm in diameter). About 100 μL of electrolyte was added the coin cell. The electrolyte contained 1MLiPF6 in EC : DEC (1 : 1) with 2% (v/v) VC and 15% (v/v) fluoroethylene carbonate (FEC). After sealing, the coin cells were taken out of the glove-box and were tested in a Biologic BCS-805 cycler. The cells were cycled over a voltage range of 1–0.05 V. Each charge and discharge is followed by the 5 min rest step under zero-current conditions. The results were analyzed with the EC-lab (Biologic software).

Mados E., Harpak N., Levi G., Patolsky F., Peled E, & Golodnitsky D. (2021). Synthesis and electrochemical performance of silicon-nanowire alloy anodes. RSC Advances, 11(43), 26586-26593.

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Ru/RuO2 Catalyst Electrochemical Evaluation

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Electrochemical studies were conducted using a three-electrode cell connected to an electrochemical analyzer (Biologic, EC-LAB Co.). Ag/AgCl (saturated with 3 M KCl, Aldrich Chemical Co.) and a graphite rod with a diameter of 6 mm and a length of 102 mm (Pine, 99.9995%) were used as a reference electrode and a counter electrode, respectively. 1.0 M KOH solution was used as an electrolyte. Working electrodes were fabricated as follows. Ru/RuO₂ catalysts (5 mg) were well dispersed in a mixture of Nafion solution (5%, 20 μL) and isopropyl alcohol (0.98 mL) through sonication. The resultant slurry ink (20 μL) was loaded on a glassy carbon rotating electrode (RDE, area of 0.164 cm², Pine). The loading amount of Ru/RuO₂ catalysts was calculated to be 0.61 mg cm⁻². The electrocatalytic performance of Ru/RuO₂ materials for the OER was investigated through linear sweep voltammetry with iR correction, a scan rate of 5 mV s⁻¹, and a rotation speed of 1600 rpm at room temperature. The 1.0 M KOH electrolyte was purged with O₂ gas during measurements. Applied potentials (E) reported in this work were referenced to the reversible hydrogen electrode (RHE) through standard calibration with the following equation: E (vs. RHE) = E (vs. Ag/AgCl) + E_Ag/AgCl (= 0.197 V) + 0.0592 pH = E (vs. Ag/AgCl) + 1.0258 V.

Cho K., Jang J.Y., Ko Y.J., Myung Y, & Son S.U. (2023). Hollow Ru/RuO2 nanospheres with nanoparticulate shells for high performance electrocatalytic oxygen evolution reactions. Nanoscale Advances, 6(3), 867-875.

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Electrochemical Characterization and SEM Imaging

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All electrochemical
experiments
were conducted with a VSP-300 potentiostat from BioLogic, controlled
with their proprietary software EC-Lab. Where applicable, gas flow
rates were controlled via mass flow controllers from Brooks (SLA5850)
via their proprietary software. Data editing and plotting were done
using the software Igor. The scanning electron microscope was operated
under high vacuum (≤8 × 10^–6 mbar),
with micrographs collected with beam settings of 15 kV and 0.40 nA
utilizing an Everhart–Thornley detector. SEM images have been
subjected to post-acquisition editing in Photoshop, possibly having
been (a) rotated, (b) cropped, and/or (c) having had their contrast
and brightness adjusted.

Raaijman S.J., Arulmozhi N, & Koper M.T. (2021). Morphological Stability of Copper Surfaces under Reducing Conditions. ACS Applied Materials & Interfaces, 13(41), 48730-48744.

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