The largest database of trusted experimental protocols

2 protocols using cd172a

1

Lymphocyte Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
After the liver tissue was gently crushed, the lymphocytes were obtained using percoll gradient purification [19 (link)]. Then, the cell suspension was stained for viability analysis, and markers including CD45, CD47, CD172a, CD11c, I-A/I-E, TNF-α, and IL-12 p40 (eBioscience, San Diego, CA).
+ Open protocol
+ Expand
2

Isolation and Characterization of Mouse Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies including anti-mouse TCRβ, CD4, CD8, CD44, CD62L, H-2Kb, H-2Kd, CD122, CD69, CD137, MHC-II, CD86, CD70, CD24, CD172a, and B220 were purchased from eBioscience. CD90.2 microbeads and negative mouse CD8+ T cells isolation kits were purchased from Miltenyi Biotec and Stem Cell Company respectively. The mouse CD11c+ cell isolation kits for CD11c+ negative selection were purchased from Stem Cell Company.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!