Gentra systems autopure ls

Genomic DNA from whole cord blood cells was extracted by the HiBR laboratory. Clotted cord blood was centrifuged through a Clotspin Basket (Qiagen) at 2,000 × g for 5 min to disperse the clot. Three volumes of Red Blood Cell (RBC) Lysis Solution (Qiagen) were added to one volume of dispersed cord blood. The samples were briefly vortexed, shaken for 15 min at room temperature then centrifuged as above to pellet leukocytes and clot particulates. The pellet was resuspended in Cell Lysis Solution (Qiagen) containing Proteinase K (Qiagen) at 20 mg/ml and then incubated at 55°C overnight. The Gentra Systems Autopure LS (Qiagen) was used to extract the DNA from the sample using the Compromised Cell Lysate Protocol. The purified DNA solution was then incubated in a 65°C water bath for 1 h and shaken at room temperature overnight.