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Rpmi 8226 media

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RPMI 8226 media is a cell culture medium designed to support the growth and maintenance of human multiple myeloma cells. It provides the necessary nutrients and growth factors for the proliferation of these specific cell lines.

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Lab products found in correlation

Xf24 extracellular flux analyzer, by Agilent Technologies (2 mentions)

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RPMI media (5 citations)

2 protocols using rpmi 8226 media

1

Measuring Oxygen Consumption in LN229 Cells

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Oxygen consumption of LN229 cells was measured using an XF24 Extracellular Flux Analyzer (Seahorse Bioscience). 60,000 cells were plated per well the night before the experiments, and RPMI 8226 media (US Biological #9011) containing 2 mM glutamine and 10 mM glucose without serum was used as the assay media. Oxygen consumption measurements were normalized based on protein concentration obtained from the same plate used for the assay.
Kim D., Fiske B.P., Birsoy K., Freinkman E., Kami K., Possemato R., Chudnovsky Y., Pacold M.E., Chen W.W., Cantor J.R., Shelton L.M., Gui D.Y., Kwon M., Ramkissoon S.H., Ligon K.L., Kang S.W., Snuderl M., Heiden M.G, & Sabatini D.M. (2015). SHMT2 drives glioma cell survival in the tumor microenvironment but imposes a dependence on glycine clearance. Nature, 520(7547), 363-367.
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2

Measuring Oxygen Consumption in LN229 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oxygen consumption of LN229 cells was measured using an XF24 Extracellular Flux Analyzer (Seahorse Bioscience). 60,000 cells were plated per well the night before the experiments, and RPMI 8226 media (US Biological #9011) containing 2 mM glutamine and 10 mM glucose without serum was used as the assay media. Oxygen consumption measurements were normalized based on protein concentration obtained from the same plate used for the assay.
Kim D., Fiske B.P., Birsoy K., Freinkman E., Kami K., Possemato R., Chudnovsky Y., Pacold M.E., Chen W.W., Cantor J.R., Shelton L.M., Gui D.Y., Kwon M., Ramkissoon S.H., Ligon K.L., Kang S.W., Snuderl M., Heiden M.G, & Sabatini D.M. (2015). SHMT2 drives glioma cell survival in the tumor microenvironment but imposes a dependence on glycine clearance. Nature, 520(7547), 363-367.
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