Monoclonal rat anti-VANGL2 (2G4 monoclonal antibody) was generated as described21 (link). Anti-p62/SQSTM1 antibodies used in the study are as follows: mouse antibody (BD Biosciences 610833) or M01, clone 2C11, for EM experiments (Abnova: H00008878-M01) and guinea-pig polyclonal anti-p62 (Progen Biotechnik, GP62-C). Other antibodies used are as follows: rabbit antibody to LC3 (MBL Technology: PM036), rabbit antibody to GFP Abcam (ab290) and mouse antibody to α-tubulin (Sigma: B512), rabbit antibody to phospho-Thr183/Tyr185 SAPK/JNK (Cell Signalling: 9251) and SAPK/JNK (Cell Signalling: 9252), rabbit anti-β-catenin antibody (Santa Cruz Biotechnology: H102), mouse antibody to glyceraldehyde-3-phosphate dehydrogenase (Abcam: ab9484), mouse monoclonal ubiquitin antibody (Life Sensors: FK2 AB120) and secondary antibodies coupled to horseradish peroxidase (Jackson Immunoresearch). Alexa Fluor-conjugated antibodies were purchased from Molecular Probes, Invitrogen. Antibodies were used according to the recommendations of the manufacturers or associated references.
Mouse antibody to α tubulin
Manufactured by Merck Group
Mouse antibody to α-tubulin is a laboratory reagent used to detect and visualize the α-tubulin protein, a key component of the cytoskeleton in eukaryotic cells. This antibody can be used in various immunodetection techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the distribution and expression of α-tubulin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Secondary antibodies coupled to horseradish peroxidase, by Jackson ImmunoResearch (1 mentions)
Rabbit anti β catenin antibody, by Santa Cruz Biotechnology (1 mentions)
Gp62 c, by Progen Biotechnik (1 mentions)
Recombinant human wnt5a, by R&D Systems (1 mentions)
Horseradish peroxidase, by Jackson ImmunoResearch (1 mentions)
2 protocols using mouse antibody to α tubulin
1
Antibodies for Protein Detection
2
Antibodies for PTK7 and WNT5A Signaling
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Monoclonal rat and polyclonal rabbit antibodies to PTK7 (1G9 and KN) were generated in the laboratory. Other antibodies used in this study according to the recommendations of the manufacturers were as follows: mouse antibody to α-tubulin (Sigma, catalog no. B512), rabbit antibody to Thr-183/Tyr-185 SAPK (stress-activated protein kinase)/JNK (Cell Signaling Technology, catalog no. 9251), polyclonal rabbit antibody to JNK (Santa Cruz Biotechnology, catalog no. sc-571), monoclonal mouse antibody to FLAG (Sigma, catalog no. F3165), monoclonal mouse antibody to MYC (Santa Cruz Biotechnology, catalog no. 9E10), monoclonal mouse antibody to HA (Covance, catalog no. MMS-101R-500), and secondary antibodies coupled to horseradish peroxidase (Jackson ImmunoResearch Laboratories). Recombinant human WNT5A was purchased from R&D Systems (catalog no. 645-WN-010).
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