Deoxyribonucleoside triphosphates

Manufactured by New England Biolabs

Sourced in United States

Deoxyribonucleoside triphosphates are the building blocks of DNA. They are the four nucleotides (adenine, guanine, cytosine, and thymine) in their triphosphate form, which are required for DNA synthesis and amplification.

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Lab products found in correlation

Betaine, by Merck Group (2 mentions) Evagreen, by Biotium (2 mentions) Mgso4, by New England Biolabs (2 mentions) Transcriptaid t7 high yield transcription kit, by Thermo Fisher Scientific (2 mentions) Dna clean concentrator 5 kit, by Zymo Research (2 mentions) Thiazolyl blue tetrazolium bromide mtt, by Merck Group (2 mentions) Glycogen, by Thermo Fisher Scientific (2 mentions) Ribonucleotide solution mix, by New England Biolabs (2 mentions) T7 endonuclease 1, by New England Biolabs (2 mentions) Dneasy blood tissue kit, by Qiagen (2 mentions) Super gelred, by US Everbright (2 mentions) Streptococcus pyogenes, by New England Biolabs (1 mentions) Nanodrop 2000c, by Thermo Fisher Scientific (1 mentions) 4 2 hydroxyethyl piperazine 1 ethanesulfonic acid hepes, by Merck Group (1 mentions) Pharos fx molecular imager, by Bio-Rad (1 mentions) Fe20 fiveeasy ph, by Mettler Toledo (1 mentions)

Spelling variants of this product

Deoxyribonucleoside 5′-triphosphate mixture (dNTPs) (3 citations)

4 protocols using deoxyribonucleoside triphosphates

RT-LAMP Assay for Nucleic Acid Detection

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The RT-LAMP assay was adapted from the work by Curtis et al. [17 (link)]. Reaction concentrations of buffers
were 1× isothermal amplification buffer, 1.4m mol·L
⁻¹ deoxyribonucleoside triphosphates (dNTPs), and 10
mmol·L⁻¹ MgSO₄ from New England
Biolabs, and 0.4 mol ·L ⁻¹ betaine from
Sigma-Aldrich. In some cases, where indicated, 0.8
mol·L⁻¹ betaine was used. These reaction
buffer components were prepared in appropriate ratios in bulk and stored at
−20 °C between experiments. Enzymes and DNA intercalating
dye were added separately to this buffer mix for a complete master mix that
was freshly prepared for each experiment. The RT-LAMP enzymes used in the
reaction were 0.64 U· µL
⁻¹Bst 2.0 DNA polymerase and 0.08
U· µL⁻¹ AMV reverse transcriptase fro m
New Engl and Biolabs. 1× EvaGreen from Biotium, a double-stranded
DNA (dsDNA) intercalating dye, was included in the reaction for the
detection of reaction products.

Damhorst G.L., Duarte-Guevara C., Chen W., Ghonge T., Cunningham B.T, & Bashir R. (2015). Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip from Whole Blood. Engineering (Beijing, China), 1(3), 324-335.

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RT-LAMP Assay for Nucleic Acid Detection

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Streptococcus pyogenes Genetic Manipulation

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Streptococcus pyogenes (product # M0646), T7 Endonuclease I (product # M0302), Ribonucleotide solution mix (NTPs) and deoxy-ribonucleoside triphosphates (dNTPs) were purchased from New England Biolabs (USA). Transcript Aid T7 High Yield Transcription kit (product # K0441) and Glycogen (product # R0561) were purchased from Thermo Fisher Scientific. Pyrobest™ DNA Polymerase and PrimeSTAR HS DNA Polymerase were purchased from TaKaRa Shuzo Co. Ltd. (Tokyo, Japan). DNA Clean & Concentrator™-5 kit (product # D4014) was purchased from Zymo Research Corp. The DNeasy Blood & Tissue Kit was purchased from QIAGEN. The oligonucleotides at HPLC purity were obtained from TaKaRa company (Dalian, China). The nucleic acid stains Super GelRed (NO.: S-2001) was bought from US Everbright Inc. (Suzhou, China). Thiazolyl Blue Tetrazolium Bromide (MTT, CAS # 298-93-1) were purchased from Sigma-Aldrich Inc. (Shanghai, China). DPBS (CAS # 63995-75-5) was purchased from TCI (Shanghai) Development Co., Ltd.

Li Y., Zhou H., Chen S., Li Y., Guo Y., Chen X., Wang S., Wang L., Gan Y., Zhang S., Zheng Y.Y., Sheng J., Zhou Z, & Wang R. (2024). Bioorthogonal labeling and profiling of N6-isopentenyladenosine (i6A) modified RNA. Nucleic Acids Research, 52(6), 2808-2820.

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CRISPR-Cas9 Enzymatic Cleavage Protocol

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Cas9 Nuclease, Streptococcus pyogenes (product# M0646), T7 Endonuclease I (product# M0302) Ribonucleotide solution mix (NTPs) and deoxy-ribonucleoside triphosphates (dNTPs) were purchased from New England Biolabs (USA). Transcript Aid T7 High Yield Transcription kit (product# K0441) and Glycogen (product# R0561) were purchased from Thermo Fisher Scientific. Pyrobest™ DNA Polymerase and PrimeSTAR HS DNA Polymerase were purchased from TaKaRa Shuzo Co. Ltd. (Tokyo, Japan). DNA Clean & Concentrator™-5 kit (product# D4014) was purchased from Zymo Research Corp. The DNeasy Blood & Tissue Kit was purchased from QIAGEN. The oligonucleotides at HPLC purity were obtained from TaKaRa company (Dalian, China). The nucleic acid stains Super GelRed (NO.: S-2001) was bought from US Everbright Inc. (Suzhou, China). DPBA (CAS# 17261-28-8), TCEP (CAS# 51805-45-9), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, CAS# 7365-45-9) and Thiazolyl Blue Tetrazolium Bromide (MTT, CAS# 298-93-1) were purchased from Sigma-Aldrich Inc. (Shanghai, China). DPBS (CAS# 63995-75-5) was purchased from TCI Development Co., Ltd (Shanghai). The pH was measured with Mettler Toledo, FE20-Five Easy™ pH (Mettler Toledo, Switzerland). The concentration of DNA or RNA was quantified by NanoDrop 2000c (Thermo Fisher Scientific, USA). Gel Imaging was performed using Pharos FX Molecular imager (Bio-Rad, USA).

Wang S.R., Wu L.Y., Huang H.Y., Xiong W., Liu J., Wei L., Yin P., Tian T, & Zhou X. (2020). Conditional control of RNA-guided nucleic acid cleavage and gene editing. Nature Communications, 11, 91.

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