Immulite 2000 double system platform

Blood samples were taken under standardized conditions between 8:15 and 9:00 a.m. after at least 12 hours fasting by puncturing the cubital vein. Before the extraction, fasting was confirmed by the child and his parents. When it was anticipated that the transfer of samples to the laboratory would take longer than 75 minutes they were centrifuged in situ and transferred refrigerated. Three aliquots of each sample were frozen, one for the determination of biochemical variables investigated in this study and the others for future analyses of which the parents were aware [19 (link)].
The following biochemical parameters were determined: triglycerides (TG), GPO-PAP (glycerolphosphate oxidase, peroxidase enzymatic method) and c-direct plus HDL.
Lipid profiles were determined over a 48-hour period using a MODULAR DPP system from Roche Diagnostics, and insulin levels were assessed using an Immulite 2000 double system platform of Siemens.

Blood samples were taken by puncturing the cubital vein, under standardized conditions, between 8:15 and 9:00 A.M., with the participant having fasted at least 12 hours beforehand. When the transfer of the samples to the laboratory took longer than 75 min, they were centrifuged in situ and transferred refrigerated.
The following variables were determined according to biochemical parameters: triglycerides (TGs) (glycerol phosphate oxidase-peroxidase [GPO-PAP] enzymatic method) and c-direct plus high-density lipoprotein (HDL). Lipid profile determinations were made on a weekend, in a MODULAR DPP from Roche Diagnostics, and insulin levels were assessed using an Immulite 2000 double system platform of Siemens.

Blood samples were taken under standardized conditions between 8:15 and 9:00 a.m., after at least 12 hours fasting by puncturing the cubital vein. Before the extraction, fasting was confirmed by the child and their parents. When it was anticipated that the transfer of samples to the laboratory would take longer than 75 minutes, they were centrifuged in situ and transferred refrigerated. Three aliquots of each sample were frozen, one for the determination of biochemical variables investigated in this study and the others for future analyses of which the parents were informed. [24 (link)]
The following biochemical parameters were determined: insulin, triglycerides (TG) and HDL-c. Lipid profiles were determined over a 48-hour period using a MODULAR DPP system from Roche Diagnostics, and insulin levels were assessed using an Immulite 2000 double system platform of Siemens. The coefficients of variation between batches were as follows: insulin, 2.47 to 3.34%; triglyceride, 1.12%; and HDL-c, 2.93 to 2.07%.