Hrp anti mouse

Manufactured by Jackson ImmunoResearch

Sourced in United Kingdom, Panama

HRP anti-mouse is a horseradish peroxidase (HRP) conjugated secondary antibody that binds to mouse primary antibodies. It is used as a detection reagent in various immunoassays and immunohistochemical techniques.

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5 protocols using hrp anti mouse

Antibody Validation for Protein Analysis

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The following antibodies were used: α-Tubulin (T9026); Acetyl-Histone H4 (06–866 lot#2554112, EMD Millipore (Billerica, MA)); CHD4 (A301-081A, Bethyl Laboratories (Montgomery, TX)); FITC anti-mouse (715-095-150 lot#115855, Jackson ImmunoResearch (West Grove, PA)); FLAG M2 (F1804 lot#SLBG5673V and lot#124K6106); FLAG M2 (F3165 lot#SLBL1237V); HDAC2 (ab7029, lot#GR88809-7, Abcam (Cambridge, UK)); HRP anti-mouse (115-035-146, Jackson ImmunoResearch); MBD2 (A301-632A, Bethyl); mouse IgG (315-005-003 lot#120058, Jackson ImmunoResearch); MTA2 (ab8106 lot#GR185489-3, Abcam); TRITC anti-rabbit (711-025-152 lot#114768, Jackson ImmunoResearch); rabbit monoclonal antibodies against DUX4 (E5-5 and E14-3) were produced in collaboration with Epitomics and are described elsewhere (Geng et al., 2012 (link)).

Campbell A.E., Shadle S.C., Jagannathan S., Lim J.W., Resnick R., Tawil R., van der Maarel S.M, & Tapscott S.J. (2018). NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins. eLife, 7, e31023.

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Comprehensive Antibody Characterization Protocol

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The primary antibodies used in the present study were PLEKHA7 (HPA038610; Sigma-Aldrich), p120 (33-9600; Life Technologies), E-cadherin (610182; BD Transduction Labs), Ago2 (ab57113; Abcam), Ago2 (28550002; Novus Biologicals), Ago2 (AP5281; ECM Biosciences), pAgo2 S387 (AP5291; ECM Biosciences), pAgo2 Y393 (AP5311; ECM Biosciences), GW182 (sc-56314 and sc-377006; Santa Cruz Biotechnology), PABPC1 (04-1467), Dicer (SAB4200087; Sigma-Aldrich), Dicer (sc-136979; Santa Cruz Biotechnology), TRBP (ABE623; Millipore), SOX2 (2748; Cell Signaling Technology), JUN (9165; Cell Signaling Technology), MYC (13-2500; Life Technologies), Nezha (SAB4200415; Sigma-Aldrich), and Actin (A2066; Sigma-Aldrich). Working dilutions were 1:50 to 1:500 for immunofluorescence and 1:500 to 1:2,000 for Western blot.
The secondary antibodies used in the present study were HRP anti–mouse (715-035-150; Jackson ImmunoResearch Laboratories), HRP anti–rabbit (711-035-152; Jackson ImmunoResearch Laboratories), Alexa Fluor 488 anti–mouse (A-11029), Alexa Fluor 488 anti–rabbit (A11034; Life Technologies), Alexa Fluor 594 anti–mouse (A-11005; Life Technologies), and Alexa Fluor 594 anti–rabbit (A-11037; Life Technologies). Working dilutions were 1:500 for immunofluorescence and 1:2,000 for Western blot.

Kourtidis A., Necela B., Lin W.H., Lu R., Feathers R.W., Asmann Y.W., Thompson E.A, & Anastasiadis P.Z. (2017). Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling. The Journal of Cell Biology, 216(10), 3073-3085.

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Western Blot Analysis of Tau Pathology

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Protein measurements were performed using adult head lysates and imaged using an Amersham Imager 680 or Vilber Lourmat Fusion FX. Densitometric analyses of western blots were quantitated using the Gels tool of ImageJ. The following antibodies were used: mouse anti-cleaved-Tau (Asp421) clone C3 (Millipore; 36-017) 1:1000, rabbit anti-TauC (1:20,000), rabbit anti-PHF-1 (Sigma) 1:2000, monoclonal anti-phospho-Tau (Ser202, Thr205) (AT8) (Thermo Fisher) 1:2000, monoclonal anti-phospho-Tau (Thr212, Ser214) (AT100) (Thermo Fisher) 1:2000, monoclonal anti-Spectrin (DSHB) 1:1000, monoclonal anti-alpha tubulin (Sigma) 1:2000, mouse anti-HA (Covance) 1:1000, guinea pig anti-Vps26 (gift from Hugo Bellen) (1:4000), HRP anti-mouse (Jackson Immunoresearch) (1:20,000), HRP anti-rabbit (Jackson ImmunoResearch) (1:20,000), HRP anti-guinea pig (1:20,000). Additional details on head lysate preparation, immunoprecipitation, and antibody staining procedures are described in supplemental methods.

Asadzadeh J., Ruchti E., Jiao W., Limoni G., MacLachlan C., Small S.A., Knott G., Santa-Maria I, & McCabe B.D. (2022). Retromer deficiency in Tauopathy models enhances the truncation and toxicity of Tau. Nature Communications, 13, 5049.

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Comprehensive Antibody Panel for Cell Signaling

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Primary antibodies used in the present study are: PLEKHA7 (Sigma, St Louis, MO, cat# HPA038610), p120 (EMD Millipore, Burlington, MA, cat# 05-1567), E-cadherin (Life Technologies, Carlsbad, CA, cat# 18-0223), Ago2 (Abcam, Cambridge, MA, cat# ab57113; Novus Biologicals, Centennial CO, cat# 28550002; ECM Biosciences, Versailles, KY, cat# AP5281), GW182 (Santa Cruz, Dallas, TX, cat# sc-56314), DROSHA (Life Technologies, Carlsbad, CA, cat# PA25-97013; Life Technologies, Carlsbad, CA, cat# MA5-3281; Abcam, Cambridge, MA, cat# Ab12216; Abnova, Taipei, Taiwan, cat# PAB7156), DGCR8 (Sigma, St Louis, MO, cat# HPA019965; Abnova, Taipei, Taiwan, cat# H00054487-M01), Actin (Sigma, St Louis, MO, cat# A2066). Working dilutions: 1:50-1:500 for immunofluorescence, 1:500-1:2000 for Western blot. Secondary antibodies used in the present study: HRP-anti-mouse (Jackson ImmunoResearch, West Grove, PA, cat# 715-035-150), HRP-anti-rabbit (Jackson ImmunoResearch, West Grove, PA, cat# 711-035-152), Alexa 488 anti-mouse (Life Technologies, Carlsbad, CA, cat# A-11029), Alexa 488 anti-rabbit (Life Technologies, Carlsbad, CA, cat# A11034), Alexa 594 anti-mouse (Life Technologies, Carlsbad, CA, cat# A-11005), Alexa 594 anti-rabbit (Life Technologies, Carlsbad, CA, cat# A-11037). Working dilutions: 1:500 for immunofluorescence, 1:2000 for Western blot.

Nair-Menon J., Daulagala A.C., Connor D.M., Rutledge L., Penix T., Bridges M.C., Wellslager B., Spyropoulos D.D., Timmers C.D., Broome A.M, & Kourtidis A. (2020). Predominant Distribution of the RNAi Machinery at Apical Adherens Junctions in Colonic Epithelia Is Disrupted in Cancer. International Journal of Molecular Sciences, 21(7), 2559.

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Western Blot Protocol for Protein Detection

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Lysates were analyzed by SDS-PAGE followed by transfer to PVDF membranes, as described in (22) .
Primary antibodies: anti-CRD-BP (Cell Signaling cat#8482; RRID:AB_11179079), diluted 1:1000; antivinculin (Millipore cat#05-386; RRID:AB_309711) diluted 1:5000; anti-GAPDH (Cell Signaling cat#2118; RRID:AB_561053) diluted 1:3000-1:5000. Secondary antibodies: HRP anti-mouse (Jackson Immunoresearch cat# 715-035-151; RRID:AB_2340771); HRP anti-rabbit (Invitrogen cat# G-21234 RRID:AB_2536530) diluted 1:5000 or HRP mouse anti-rabbit IgG (conformation-specific) (Cell Signaling cat#L27A9; RRID:AB_10892860). All antibodies were diluted in 5% milk in TBS-Tween.

Fakhraldeen S.A., Berry S., Beebe D.J., Roopra A., Spiegelman V.S., & Alexander C.M. (2021). Enhanced Immunoprecipitation Techniques for the Identification of RNA Binding Protein Partners: CRD-BP interactions in mammary epithelial cells.

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